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Use of an affinity immunosorbent for the isolation of lactoferrin from breast milk serum

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Abstract

An affinity or biospecific method of isolating an iron-binding glycoprotein — lactoferrin — from breast milk is described which permits in a single chromatographic operation the isolation of lactoferrin homogeneous according to the results of electrophoresis in polyacrylamide gel. The affinity method of isolating lactoferrin is based on the use of a specific immunosorbent consisting of antibodies to lactoferrin covalently conjugated with cyanogen-bromide-activated Sepharose 4B.

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Scientific-Research Institute of Hematology and Blood Transfusion Ministry of Health of the Uzbek SSR, Tashkent. Translated from Khimiya Priordnykh Soedinenii, No. 6, pp. 749–750, November–December, 1986.

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Buglanov, A.A. Use of an affinity immunosorbent for the isolation of lactoferrin from breast milk serum. Chem Nat Compd 22, 697–698 (1986). https://doi.org/10.1007/BF00598355

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  • DOI: https://doi.org/10.1007/BF00598355

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