Advertisement

Chemistry of Natural Compounds

, Volume 22, Issue 6, pp 697–698 | Cite as

Use of an affinity immunosorbent for the isolation of lactoferrin from breast milk serum

  • A. A. Buglanov
Article
  • 19 Downloads

Abstract

An affinity or biospecific method of isolating an iron-binding glycoprotein — lactoferrin — from breast milk is described which permits in a single chromatographic operation the isolation of lactoferrin homogeneous according to the results of electrophoresis in polyacrylamide gel. The affinity method of isolating lactoferrin is based on the use of a specific immunosorbent consisting of antibodies to lactoferrin covalently conjugated with cyanogen-bromide-activated Sepharose 4B.

Keywords

Cyanogen Cyanogen Bromide Monospecific Antibody Affinity Sorbent Milk Serum 

Literature cited

  1. 1.
    P. Querinjean, P. L. Masson, and J. F. Heremans, Eur. J. Biochem.,29, 420 (1971).CrossRefGoogle Scholar
  2. 2.
    N. Moguilevsky, L. A. Retegui, and P. L. Masson, Biochim. J.,229, 352 (1985).Google Scholar
  3. 3.
    L. Blackberg and O. Hernell, FEBS Lett.,109, 108 (1980).CrossRefGoogle Scholar
  4. 4.
    A. A. Buglanov, Kh. M. Kazakbaeva, et al., Med. Zh. Uzbek.,6, 24 (1985).Google Scholar
  5. 5.
    H. Friemel, Immunological Methods [Russian translation], Moscow (1970).Google Scholar
  6. 6.
    K. Weber and M. Osborn, J. Biol. Chem.,244, 4406 (1969).Google Scholar
  7. 7.
    B. G. Belen'kii, et al., Dokl. Akad. Nauk SSSR,172, 91 (1967).Google Scholar
  8. 8.
    CNBr-Activated Sepharose 4B and Con-A-Sepharose. Pharmacia Fine Chemicals AB, Uppsala, Sweden (1972).Google Scholar

Copyright information

© Plenum Publishing Corporation 1987

Authors and Affiliations

  • A. A. Buglanov

There are no affiliations available

Personalised recommendations