Determination of protein in solutions from absorption in the ultraviolet region

Abstract

A comparison of spectrophotometric methods of determining protein in solutions from absorption in the UV region has shown the advantage of a procedure based on measuring the optical densities at 235 and 280 nm and the possibility of its use for the analysis of plant proteins.

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Literature cited

  1. 1.

    O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, J. Biol. Chem.,193, 265 (1951).

    CAS  Article  Google Scholar 

  2. 2.

    R. K. Scopes, Protein Purification, Principles and Practice, Springer, New York (1985), p. 265.

    Google Scholar 

  3. 3.

    I. A. Gorina, “Critical review of modern methods of determining protein,” [in Russian], Moscow (1979); Paper Deposited in VINITI [All-Union Institute of Scientific and Technical Information], No. 1803-79 from Vestnik Mosk. Univ.

  4. 4.

    K. Gerald, M. Chirs, and M. Brad. Anal. Biochem.,148, 451 (1985).

    Article  Google Scholar 

  5. 5.

    A. P. Demchenko, Ultraviolet Spectrophotometry and Protein Structure [in Russian], Naukova Dumka, Kiev (1981), p. 153.

    Google Scholar 

  6. 6.

    T. A. Beweley, Anal. Biochem.,123, 55 (1982).

    Article  Google Scholar 

  7. 7.

    V. S. Asatiani, New Methods of Biochemical Photometry [in Russian], Nauka, Moscow (1965), p. 134.

    Google Scholar 

  8. 8.

    M. K. Gil'manov, O. V. Fursov, and A. P. Frantsev, Methods of Purifying and Studying Plant Enzymes [in Russian], Nauka, Alma-Ata (1981).

    Google Scholar 

  9. 9.

    N. P. L'vov, G. D. Erygin, and O. I. Kamenskii, Prikl. Biokhim. Mikrobiol.,7, 99 (1971).

    CAS  Google Scholar 

  10. 10.

    L. V. L'vova, Selekts. Semenovod., No. 60, 60 (1986).

  11. 11.

    D. Maruhn, V. Wessel, and D. Paar, J. Clin. Clin. Biochem.,19, 764 (1981).

    Google Scholar 

  12. 12.

    J. L. Bailey, Techniques in Protein Chemistry, Elsevier, Amsterdam (1962) [Russian translation, Mir, Moscow (1965), p. 265].

    Google Scholar 

  13. 13.

    G. A. Kochetov, Practical Handbook on Enzymology [in Russian], Vysshaya Shkola, Moscow (1971), p. 312.

    Google Scholar 

  14. 14.

    J. R. Whitaker and P. E. Granum, Anal. Biochem.,109, 156 (1980).

    CAS  Article  Google Scholar 

  15. 15.

    Yu. V. Peruanskii and V. N. Savin, Fiziol. Biokhim. Kul'turn. Rast.,17, 390 (1985).

    CAS  Google Scholar 

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Scientific-Research Institute of Biology, Dnepropetrovsk State University. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 693–695, September–October, 1989.

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Fedenko, V.S. Determination of protein in solutions from absorption in the ultraviolet region. Chem Nat Compd 25, 590–592 (1989). https://doi.org/10.1007/BF00598081

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Keywords

  • Lysozyme
  • Chymotrypsin
  • Soybean Trypsin Inhibitor
  • Maize Starch
  • Spectrophotometric Procedure