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Filtration in surface glomeruli as regulated by flow rate through the loop of henle

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Summary

A new pressure transducer microperfusion system has been used to measure quantitatively SN GFR, early proximal free flow pressure and stop flow pressure of varying flow rates through the loop of Henle in the range of 0 to 50 nl/min. Perfusing the loop with an isoosmolal artificial tubular fluid at physiological flow rates SN GFR was 19.9±1.1 and 27.7±1.0 nl/min in two different strains of antidiuretic rats. SN GFR increased when loop perfusion was interrupted. The question whether a feedback control mechanism of SN GFR is operative in the rat kidney was evaluated in further experiments in which early proximal tubular pressure was measured in functionally isolated proximal convolutions in vivo under conditions of constant flow as well as stopped flow. Experiments in which perfusion rate through the loop of Henle was varied demonstrated the existence of a feedback signal which originates downstream of the late proximal convolution and which affects filtration into individual early proximal segments. This feedback mechanism exhibited an asymmetrical behaviour: Elevation of loop perfusion above the control value resulted in an early proximal pressure drop, under simulated free flow conditions as well as under stop flow conditions. In contrast lowering of perfusion rate below the predetermined physiological value had no significant effect on early proximal pressures.

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Abbreviations

FFP:

free flow pressure

P a :

blood pressure

P G :

mean glomerular capillary pressure

PCT:

proximal convoluted tubule

P tub :

intratubular hydrostatic pressure

SFP:

stop flow pressure

SN GFR:

single nephron glomerular filtration rate

TF:

tubular fluid

TF/P:

concentration ratio between tubular fluid and plasma

V(TF) :

collected volume of tubular fluid, flow rate

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Authors and Affiliations

  1. Institut für Klinische Physiologie, Klinikum Steglitz Freie Universität Berlin, Berlin, Germany

    K. Hierholzer, R. Müller-Suur, H. -U. Gutsche, M. Butz & Ingrid Lichtenstein

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  1. K. Hierholzer
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  2. R. Müller-Suur
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  3. H. -U. Gutsche
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  4. M. Butz
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  5. Ingrid Lichtenstein
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Hierholzer, K., Müller-Suur, R., Gutsche, H.U. et al. Filtration in surface glomeruli as regulated by flow rate through the loop of henle. Pflugers Arch. 352, 315–337 (1974). https://doi.org/10.1007/BF00585685

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  • DOI: https://doi.org/10.1007/BF00585685

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