Chemistry of Natural Compounds

, Volume 18, Issue 3, pp 359–362 | Cite as

Quantitative analysis of β-exotoxin in insecticidal preparations by high-performance anion-exchange chromatography

  • S. N. Bubenshchikova
  • V. K. Kagramanova
  • L. A. Baratova
  • É. B. Danilova
  • E. B. Kruglyak
Article

Abstract

A quantitative method is proposed for determining β-exotoxin, which is a product of the vital activity of certain serotypes ofBac. thuringiensis. The method is based on the use of high-performance anion-exchange chromatography. The chromatography of β-exotoxin-containing preparations was performed on a column (1.6 × 150 mm) containing type 2632 anion-exchange resin (Hitachi) in a concentration gradient of HCl and NaCl. The time of analysis amounted to 34 min. The method permits the analysis of β-exotoxin in insecticidal preparations in an amount of 1 µg and upwards.

Keywords

Culture Liquid Musca Domestica Bacterial Preparation Dephosphorylated Form Gradient Curf 

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Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • S. N. Bubenshchikova
  • V. K. Kagramanova
  • L. A. Baratova
  • É. B. Danilova
  • E. B. Kruglyak

There are no affiliations available

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