Chemistry of Natural Compounds

, Volume 18, Issue 2, pp 148–151 | Cite as

Modified method for the quantitative determination of certain deoxy sugars in lipopolysaccharides

  • T. I. Burtseva
  • Yu. S. Ovodov
Article
  • 39 Downloads

Abstract

An alkaline modification of the 2-thiobarbituric (2-TBA) method for the rapid determination of 2-keto-3-deoxy-D-mannooctonic acid (KDO) and of 3,6-dideoxyhexoses (3,6-DDHs) in hydrolysates of lipopolysaccharides is described. The modification is based on the performance of the periodate-2-TBA color reaction with the subsequent decomposition of the KDO chromophore by alkaline treatment and measurement of the optical densities of the solutions before and after this treatment. It has been found that the modification described is distinguished by high sensitivity, specificity, and reproducibility of the results. The standard deviation calculated from six determinations amounted to 2.84% for KDO and 3.93% for 3,6-DDHs. The method is simple to perform and needs only simple equipment and a small amount of reagents.

Keywords

Xylose Periodate Uronic Acid Galacturonic Acid Alkaline Treatment 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. 1.
    T. F. Solov'eva and Yu. S. Ovodov, Usp. Sovrem. Biol.,90, 62 (1980).PubMedGoogle Scholar
  2. 2.
    V. S. Waravdekar and L. D. Saslow, J. Biol. Chem.,234, 1945 (1959); A. Weissbach and J. Hurwitz, J. Biol. Chem.,234, 705 (1959).PubMedGoogle Scholar
  3. 3.
    L. Warren, J. Biol. Chem.,234, 1971 (1959).Google Scholar
  4. 4.
    R. W. Hendler and A. H. Burgess, Anal. Biochem.,47, 109 (1972).CrossRefGoogle Scholar
  5. 5.
    T. I. Burtseva, L. I. Glebko, and Yu. S. Ovodov, Anal. Biochem.,64, 1 (1975); T. I. Burtseva, L. I. Glebko, and Yu. S. Ovodov, J. Chromatogr.,131, 464 (1977).CrossRefGoogle Scholar
  6. 6.
    R. Kuhn and P. Lutz, Biochem. Z.,338, 554 (1963).PubMedGoogle Scholar
  7. 7.
    M. A. Ghalambor, E. M. Levine, and E. C. Heath, J. Biol. Chem.,241, 3207 (1966).PubMedGoogle Scholar
  8. 8.
    Yu. S. Ovodov, R. P. Gorshkova, and S. V. Tomshich, Immunochemistry,13, 581 (1976).CrossRefGoogle Scholar
  9. 9.
    G. Ekborg and S. Svensson, Acta. Chem. Scand.,27, 1437 (1973); M. C. Fouquey, M. J. Polonsky, and E. Lederer, Bull. Soc. Chim. Fr.,6, 808 (1959).CrossRefGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • T. I. Burtseva
  • Yu. S. Ovodov

There are no affiliations available

Personalised recommendations