Chemistry of Natural Compounds

, Volume 19, Issue 6, pp 675–678 | Cite as

Chromatographic separation of isoflavones

  • I. I. Ozimina
  • A. B. Bandyukov


The optimum ratios of the components of the solvent systems have been determined with the aid of the simplex-grid method of experimental planning and the separation of a number of isoflavones has been carried out with the aid of these systems in thin layers of silica gel (Silufol plates); in addition, the chromatographic constants of the functional groups have been determined, which makes it possible to obtain the ηRf values of compounds under investigation in an analysis of plant material by a mathematical method.


Genistein Isoflavones Optimum Ratio Xanthone Experimental Planning 


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Literature cited

  1. 1.
    T. J. Mabry, K. R. Markham, and M. B. Thomas, The Systematic Identification of Flavonoids, Springer, New York (1970).CrossRefGoogle Scholar
  2. 2.
    L. K. Klyshev, V. A. Bandyukova, and L. S. Alyukina, Plant Flavonoids [in Russian], Alma-Ata (1978).Google Scholar
  3. 3.
    E. Stahl, Thin-Layer Chromatography, Allen and Unwin, London (1969).CrossRefGoogle Scholar
  4. 4.
    K. Egger, Z. Anal. Chem.,171, No. 2, 96 (1959).CrossRefGoogle Scholar
  5. 5.
    R. V. M. Cambell and J. Tannoch, J. Chem. Soc. Perkin Trans, Part 1, No. 19, 2222 (1973).Google Scholar
  6. 6.
    M. I. Meegan and D. M. X. Donelly, Phytochemistry,14, No. 10, 2283 (1975).CrossRefGoogle Scholar
  7. 7.
    J. L. Ingham, Z. Naturforsch.,34, No. 1–2, 159 (1979).Google Scholar
  8. 8.
    S. H. Harper, D. B. Shirley, and D. A. Taylor, Phytochemistry,15, No. 6, 1019 (1976).CrossRefGoogle Scholar
  9. 9.
    N. G. Mukeshina, in: New Ideas in the Planning of Experimental Work [in Russian], Moscow (1969), p. 191.Google Scholar
  10. 10.
    B. J. Panova, M. F. Mincheva, and D. A. Minchev, Dokl. Bolg. Akad. Nauk,25, No. 9, 1245 (1972).Google Scholar
  11. 11.
    T. A. Chemleva and N. G. Mikeshina, in: New Ideas in the Planning of Experimental Work [in Russian], Moscow, (1969), p. 191.Google Scholar
  12. 12.
    V. P. Georgievskii, Rast. Resur.,18, No. 3, 427 (1982).Google Scholar

Copyright information

© Plenum Publishing Corporation 1984

Authors and Affiliations

  • I. I. Ozimina
  • A. B. Bandyukov

There are no affiliations available

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