Abstract
Vero cell lines, usually considered anchorage-dependent, could be grown as cell-aggregates in suspension culture with serum-free media. Several different combinations of base media gave growth results above 106 cells/ml (NCTC 135:SFRE 199-1; NCTC 135:Waymouth MB 752/1; NCTC 135:RPMI 1640). Insulin was not essential for growth and Bovine Serum Albumin could be diluted out of the media if linoleic acid was present. The size and density of the aggregates formed varied depending on the media used.
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References
Durand RE (1990) Multicell spheroids as a model for cell kinetic studies. Cell Tissue Kinet. 23: 141–159.
Folkman J and Greenspan HP (1975) Influence of geometry on control of cell growth. Biochim. Biophy. Acta 417: 211–236.
Goetghebeur S and Hu W-S (1991) Microsphere-induced aggregate culture of animal cells. In: Spier RE, Griffiths JB and Meignier B (eds) Production of biologicals from animal cells in culture. pp. 423–428. Butterworth-Heinemann Ltd.
Litwin J (1991) The growth of CHO and BHK cells as suspended aggregates in serum-free medium. In: Spier RE, Griffiths JB and Meignier B (eds) Production of biologicals from animal cells in culture. pp. 429–433. Butterworth-Heinemann Ltd.
Litwin, J. (1992) The growth of Vero cells as suspended aggregates in serum-free medium. In: Spier HE, Griffiths JB and MacDonald C (eds) Animal cell technology: Developments, processes and products. pp. 414–417. Butterworth-Heinemann Ltd.
Schwachöfer JHM (1990) Multicellular tumor spheroids in radiotherapy research. Anticancer Res. 10: 963–970.
Tolbert WR, Hitt MM and Feder J (1980) Cell aggregate suspension culture for large-scale production of biomolecules. In Vitro 16: 486–490.
Trépanier P, Dugré R, Alain R and Lapierre J (1990) Growth of poliovirus using alginate entrapped-anchorage dependent Vero cells. Cytotechnology 4: 261–269.
White LA and Ades EW (1990) Growth of Vero E-6 cells on microcarriers in a cell bioreactor. J. Clin. Microbiol. 28, 283–286.
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Litwin, J. The growth of Vero cells in suspension as cell-aggregates in serum-free media. Cytotechnology 10, 169–174 (1992). https://doi.org/10.1007/BF00570893
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DOI: https://doi.org/10.1007/BF00570893