Summary
1. It has been shown that the values of Km for the esterase activity of elastase ofActinomyces rimosus and for pancreatic elastase are of the same order of magnitude.
2. The elastase ofActinomyces rimosus, unlike the pancreatic enzyme, is capable of hydrolyzing only the methyl ester of N-acetyltrialanine, and not the mono and di derivatives.
3. It has been found that in the initial stage of the hydrolysis reaction it is mainly the ester bonds that are cleaved.
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Yu. A. Rassulin, L. R. Radzhabov, E. D. Kaverzneva, V. A. Shibnev, and G. S. Erkomaishvili, Izv. Akad. Nauk SSSR, Ser. Khim., No. 3, 687 (1974).
L. R. Radzhabov, Yu. A. Rassulin, L. I. Mar'yash, and V. A. Shibnev, Bioorg. Khim.,1, 554 (1975).
D. M. Shotton, Methods in Enzymology,19, 113 (1970).
A. Gertler and T. Hofman, Can. J. Biochem.,51, 121 (1973).
A. Gertler and T. Hofman, Can. J. Biochem.,48, 384 (1970).
A. Gertler and M. Trop, Europ. J. Biochem.,19, 90 (1973).
J. Bieth and J. Meyer, Anal. Biochem.,51, 121 (1973).
S. Hestrin, J. Biol. Chem.,180, 249 (1949).
A. P. Ryle and R. R. Porter, Biochem. J.,73, 75 (1959).
H. Lineweaver and D. Burk, J. Am. Chem. Soc.,56, 658 (1934).
Additional information
Institute of Molecular Biology, Academy of Sciences of the USSR, Moscow. N. D. Zelinskii Institute of Organic Chemistry, Academy of Sciences of the USSR, Moscow. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 693–695, September–October, 1977.
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Radzhabov, L.R., Shibiev, V.A. & Rassulin, Y.A. The esterase and peptidase activities of an elastolytic enzyme of Actinomyces rimosus. Chem Nat Compd 13, 575–577 (1977). https://doi.org/10.1007/BF00569596
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DOI: https://doi.org/10.1007/BF00569596