Abstract
The fate of nitrate in sediments from seagrass (Zostera capricorni Aschers.) beds of Moreton Bay on the subtropical eastern coast of Queensland, Australia, was investigated. Added nitrate was metabolised at rates of 0.4 to 3.4 μg N cm-3 d-1 when sediments were incubated under anaerobic conditions with a large excess of nitrate. The potential rate of nitrate utilization was as rapid in sediments from subtidal bare areas as from adjacent seagrass beds. Ammonium was produced rapidly from15N-nitrate by microbial action in all the subtidal sediments examined. After 12 h of incubation, 13 to 28% of the15N initially added as labelled nitrate was detected as labelled ammonium in the sediments. Denitrification, although not measured directly, appeared to be a relatively minor fate of nitrate. Benthic microbes took up large amounts of15N but only after a delay of 6 h; this pattern could have been due to induction and synthesis of the enzymes necessary for nitrate uptake, and the assimilation of labelled ammonium. Under field conditions, assimilation by seagrasses and denitrification by bacteria were probably not significant sinks for nitrate in comparison with uptake by benthic microbes and dissimilatory reduction to ammonium.
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Boon, P. I., D. J. W. Moriarty and P. G. Saffigna: Rates of ammonium turnover and the role of amino-acid deamination in seagrass (Zostera capricorni) beds of Moreton Bay, Australia. Mar. Biol.91, 259–268 (1986)
Bremner, J. M.: Inorganic forms of nitrogen.In: Methods of soil analysis. Part 2. Chemical and microbiological properties, pp 1179–1137. Ed. by C. A. Black. Wisconsin: American Society of Agronomy 1965
Bremner, J. M. and K. Shaw: Denitrification in soil. I. Methods of investigation. J. agric. Sci., Camb51–52, 22–39 (1958)
Bulthuis, D. A. and W. J. Woelkerling: Effects ofin situ nitrogen and phosphorus enrichment of the sediments on the seagrassHeterozostera tasmanica (Martens ex Aschers.) den Hartog in Westernport. Victoria, Australia. J. exp. mar. Biol. Ecol.53, 194–207 (1981)
Buresh, R. J. and W. H. Patrick, Jr.: Nitrate reduction to ammonium and organic nitrogen in an estuarine sediment. Soil Biol. Biochem.13, 279–283 (1981)
Chao, Y.: Inorganic nitrogen transformations through the oxidation and reduction of sulfur compounds. Proc. Soil Sci. Soc. Am.31, 485–490 (1967)
Clausen, C., B. R. Bock, G. A. Peterson and R. A. Olson: The magnesium problem in nitrate determination by steam distillation. J. Soil Sci. Soc. Am44, 1326–1327 (1980)
Cole, J. A. and C. M. Brown: Nitrite reduction to ammonium by fermentative bacteria: a short circuit in the biological nitrogen cycle. Fedn eur. microbiol. Soc. (FEMS) Lett.7, 65–72 (1980)
Fenchel, T. and T. H. Blackburn: Bacteria and mineral cycling, 225 pp. London: Academic Press 1979
Focht, D. D.: Methods for analysis of denitrification in soils.In: Nitrogen in the environment, pp 433–490. Ed. by D. R. Nielsen and J. G. McDonald. New York: Academic Press 1978
Harlin, M. M. and B. Thorne-Miller: Nutrient enrichment of seagrass beds in a Rhode Island coastal lagoon. Mar. Biol.65, 221–229 (1981)
Herbert, R. A.: Nitrate dissimilation in marine and estuarine sediments.In: Sediment microbiology, pp 53–71. Ed. by D. B. Nedwell and C. M. Brown. London: Academic Press 1982
Iizumi, H. and A. Hattori: Growth and organic production of eelgrass (Zostera marina L.) in temperate waters of the Pacific coast of Japan. III. The kinetics of nitrogen uptake. Aquat. Bot.12, 245–256 (1982)
Iizumi, H., A. Hattori and C. P. McRoy: Nitrate and nitrite in interstitial waters of eelgrass beds in relation to the rhizosphere. J. exp. mar. Biol. Ecol.47, 191–201 (1980)
Kaspar, H. F.: Denitrification, nitrate reduction to ammonium, and inorganic nitrogen pools in intertidal sediments. Mar. Biol.74, 133–139 (1983)
Keeney, D. R.: The nitrogen cycle in sediment water systems. J. envirl Qual.2, 15–29 (1973)
Koike, I. and A. Hattori: Denitrification and ammonia formation in anaerobic coastal sediments. Appl. envirl, Microbiol.35, 278–282 (1978)
MacRae, I. C., R. R. Ancajas and S. Salandanan: The fate of nitrate nitrogen in some tropical soils following submergence. Soil Sci.105, 327–334 (1968)
Mann, K. H.: Nitrogen limitations on the productivity ofSpartina marshes,Laminaria kelp beds and higher trophic levels.In: Ecological processes in coastal environments, pp 363–370. Ed. by R. L. Jefferies and A. J. Davy. Oxford: Blackwell 1979
McCready, R. C. L., W. D. Gould and R. W. Barendregt: Nitrogen isotope fractionation during reduction of NO -3 to NH +4 byDesulfovibrio sp. Can. J. Microbiol.29, 231–234 (1983)
Moriarty, D. J. W., P. I. Boon, J. A. Hansen, W. G. Hunt, I. R. Poiner, P. C. Pollard, G. W. Skyring and D. C. White: Microbial biomass and productivity in seagrass beds. Geomicrobiol. J4, 21–51 (1985)
Myers, R. J. K.: The effect of sulphide on nitrate reduction in soil. Pl. Soil37, 431–433 (1972)
Nedwell, D. M.: Exchange of nitrate, and products of bacterial nitrate reduction, between seawater and sediment from a U.K. saltmarsh. Estuar., cstl Shelf Sci.14, 557–566 (1982)
Nishio, T., I. Koike and A. Hattori: Denitrification, nitrate reduction and oxygen consumption in coastal and estuarine sediments. Appl. envirl Microbiol.43, 468–653 (1982)
Nixon, S. W., C. A. Oviatt and S. S. Hale: Nitrogen regeneration and the metabolism of coastal marine bottom communities.In: The role of terrestrial and aquatic organisms in decomposition processes, pp 269–283. Ed. by J. M. Anderson and A. Macfadyen. Oxford: Blackwell 1976
Patriquin, D. G.: The origin of nitrogen and phosphorus for growth of the marine angiospermThalassia testudinum. Mar. Biol.15, 35–46 (1972)
Payne, W. J.: Reduction of nitrogenous oxides by microorganisms. Bact. Rev.37, 409–452 (1973)
Ryle, V. D., H. R. Mueller and P. Gentien: Automated analysis of nutrients in tropical seawaters. Techn. Bull., Aust. Inst. mar. Sci. (Oceanogr. Ser. No. 3)AIMS-OS-81–2, 1–24 (1981)
Ryther, J. H. and W. M. Dunstan: Nitrogen, phosphorus and eutrophication in the coastal marine environment. Science, N.Y.171, 1008–1013 (1971)
Schell, D. M.: Chemical and isotopic methods in nitrification studies.In: Microbiology 1978, pp 292–295. Ed. by D. Schlessinger. Washington: American Society for Microbiology 1978
Seitzinger, S. P., S. W. Nixon and M. E. Q. Pilson: Denitrification and nitrous oxide production in a coastal marine ecosystem. Limnol. Oceanogr.29, 73–83 (1984)
Short, F. T.: The response of interstitial ammonium in eelgrass (Zostera marina L.) beds to environmental perturbations. J. exp. mar. Biol. Ecol.68, 195–208 (1983)
Short, F. T. and C. P. McRoy: Nitrogen uptake by leaves and roots of the seagrassZostera marina L. Botanica mar.27, 547–555 (1984)
Smith, S. V.: Phosphorus versus nitrogen limitation in the marine environment. Limnol. Oceanogr.29, 1149–1160 (1984)
Sørensen, J.: Capacity for denitrification and reduction of nitrate to ammonium in a coastal marine sediment. Appl. envirl Microbiol.35, 301–305 (1978)
Sørensen, J., B. B. Jørgensen and N. P. Revsbech: A comparison of oxygen, nitrate and sulfate respiration in coastal marine sediments. Microb. Ecol.5, 105–116 (1979)
Walter, W. M., D. R. Keeney and I. R. Fillery: Inhibition of nitrification by acetylene. J. Soil. Sci. Soc. Am.43, 195–196 (1979)
Yordy, D. M. and K. L. Ruoff: Dissimilatory nitrate reduction to ammonia.In: Denitrification, nitrification and atmospheric nitrous oxide, pp 171–190. Ed. by C. C. Delwiche. New York: Wiley-Interscience 1981
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Communicated by G. F. Humphrey, Sydney
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Boon, P.L., Moriarty, D.J.W. & Saffigna, P.G. Nitrate metabolism in sediments from seagrass (Zostera capricorni) beds of Moreton Bay, Australia. Mar. Biol. 91, 269–275 (1986). https://doi.org/10.1007/BF00569443
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DOI: https://doi.org/10.1007/BF00569443