Advertisement

Chemistry of Natural Compounds

, Volume 5, Issue 6, pp 458–463 | Cite as

Successive cleavage of pepsin with trypsin and cyanogen bromide isolation of a peptide fragment belonging to the C-terminal region of pepsin

  • M. I. Lapuk
  • Yu. S. Kuznetsov
  • V. M. Stepanov
Article
  • 56 Downloads

Conclusions

1. The peptide TB has been isolated from the C-terminal fragment of pepsin.

2. The amino acid composition of this region of pepsin has been refined.

3. The sequence of nine amino acids in the peptide TB has been established.

Keywords

Amino Acid Composition Chymotrypsin Cyanogen Bromide Medicinal Preparation BrCN 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    V. I. Ostoslavskaya, I. B. Pugacheva, E. A. Vakhitova, V. F. Krivtsov, G. L. Muratova, E. D. Levin, and V. M. Stepanov, Biokhim.,33, 331, 1968.Google Scholar
  2. 2.
    R. A. Matveeva, V. F. Krivtsov, and V. M. Stepanov, Biokhim.,33, 167, 1968.Google Scholar
  3. 3.
    T. A. A. Dopheide, S. Moor, and W. H. Stein, J. Biol. Chem.,242, 1833, 1967.Google Scholar
  4. 4.
    R. N. Perham and G. M. T. Jones, European J. Biochem.,2, 84–89, 1967.CrossRefGoogle Scholar
  5. 5.
    V. M. Stepanov and T. I. Greil, Biokhim.,28, 540, 1963.Google Scholar
  6. 6.
    T. I. Vaganova, E. D. Levin, and V. M. Stepanov, Biokhim.,29, 1070, 1964.Google Scholar
  7. 7.
    V. Kostka and F. Karpenter, J. Biol. Chem.,239, 1799, 1964.PubMedPubMedCentralGoogle Scholar
  8. 8.
    J. Opienska-Blaut and M. Charesinski, Anal. Bioch.,6, 69, 1963.CrossRefGoogle Scholar
  9. 9.
    D. Walz, A. R. Fahmy, G. Pataki, A. Niederwieser, and M. Brenner, Experientia,19, 213, 1963.CrossRefGoogle Scholar
  10. 10.
    W. R. Grey and B. S. Hartley, Bioch. J., 89, 1, 59P, 1963.Google Scholar
  11. 11.
    V. M. Stepanov and V. F. Krivtsov, ZhOKh,35, 982, 1965.Google Scholar
  12. 12.
    W. R. Grey and B. S. Hartley, Bioch. J.,89, 379, 1963.CrossRefGoogle Scholar

Copyright information

© Consultants Bureau 1972

Authors and Affiliations

  • M. I. Lapuk
    • 1
  • Yu. S. Kuznetsov
    • 1
  • V. M. Stepanov
    • 1
  1. 1.Institute of the Chemistry of Natural Compounds AS USSRUSSR

Personalised recommendations