Chemistry of Natural Compounds

, Volume 5, Issue 6, pp 450–452 | Cite as

Microphotometric determination of 1-dimethylaminonaphthalene-5-sulfonylamino acids with glycine as an example

  • V. Kh. Lapuk
  • A. A. Strongina
  • M. Z. Zalesskii


1. A method for the quantitative evaluation of thin-layer chromatograms of dansylglycine by photographing them and microphotometering the negative obtained has been proposed.

2. The method is applicable to amounts of 1·10−10–1·10−9 mole of dansylglycine per spot with an accuracy of ±3%.

3. It is proposed to obtain dansyl amino acids by using dioxane as solvent, which completely prevents the parasitic formation of dansylamide.


Dioxane Dansyl Parasitic Formation Dansyl Amino Acid Dansyl Derivative 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    W. R. Gray and B. S. Hartley, Biochem. J., 89, 590, 1963.CrossRefGoogle Scholar
  2. 2.
    N. Seiler and M. Wiechmann, Z. anal. Chem., 220, 109, 1966.CrossRefGoogle Scholar
  3. 3.
    G. Pataki and E. Strasky, Chimia, 20, 361, 1966.Google Scholar
  4. 4.
    N. Seiler, G. Werner, and M. Wiechmann, Naturw., 50, 643, 1963.CrossRefGoogle Scholar
  5. 5.
    S. R. Mardashev, M. Z. Zalesskii, S. I. Pekhtereva, and L. A. Semina, Biokhim., 32, 655, 1967.Google Scholar
  6. 6.
    D. J. Neadle and R. J. Pollitt, Biochim. J., 97, 607, 1965.CrossRefGoogle Scholar
  7. 7.
    N. Seiler and M. Wiechmann, J. Chromatog., 28, 351, 1967.CrossRefGoogle Scholar
  8. 8.
    B. G. Belen'kii, E. S. Gankina, and V. V. Nesterov, DAN SSSR, 172, 91, 1967.Google Scholar
  9. 9.
    V. P. Mikulin, Handbook of Photographic Formulations [in Russian], Moscow, 113, 1960.Google Scholar

Copyright information

© Consultants Bureau 1972

Authors and Affiliations

  • V. Kh. Lapuk
    • 1
  • A. A. Strongina
    • 1
  • M. Z. Zalesskii
    • 1
  1. 1.Zelinskii Institute of Organic Chemistry, AS USSRUSSR

Personalised recommendations