Microphotometric determination of 1-dimethylaminonaphthalene-5-sulfonylamino acids with glycine as an example
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1. A method for the quantitative evaluation of thin-layer chromatograms of dansylglycine by photographing them and microphotometering the negative obtained has been proposed.
2. The method is applicable to amounts of 1·10−10–1·10−9 mole of dansylglycine per spot with an accuracy of ±3%.
3. It is proposed to obtain dansyl amino acids by using dioxane as solvent, which completely prevents the parasitic formation of dansylamide.
KeywordsDioxane Dansyl Parasitic Formation Dansyl Amino Acid Dansyl Derivative
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- 3.G. Pataki and E. Strasky, Chimia, 20, 361, 1966.Google Scholar
- 5.S. R. Mardashev, M. Z. Zalesskii, S. I. Pekhtereva, and L. A. Semina, Biokhim., 32, 655, 1967.Google Scholar
- 8.B. G. Belen'kii, E. S. Gankina, and V. V. Nesterov, DAN SSSR, 172, 91, 1967.Google Scholar
- 9.V. P. Mikulin, Handbook of Photographic Formulations [in Russian], Moscow, 113, 1960.Google Scholar