Steroids LI. The transformation of a series of 20-oxosteroids by a culture of Bacillus megaterium VNIKhFI-1
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The substrate specificity of an intact culture ofBacillus megaterium in relation to steroids of the pregnane series with a dihydroxyacetone side chain and without it, and also with steroids containing a 16α,17α-epoxy ring, has been studied.
It has been shown that a culture ofBac. megaterium performs the reduction of a 20-oxo to a 20α-hydroxy group only in the case of Δ4-3-oxo-steroids containing a 16α,17α-epoxy ring.
The necessity of a dihydroxyacetone side chain for the β-reduction of a 20-oxo group has been established.
KeywordsManganese Dioxide Epoxy Ring Deoxycorticosterone Deuterochloroform Sodium Tetrahydroborate
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