Chemistry of Natural Compounds

, Volume 4, Issue 2, pp 99–101 | Cite as

A study of the reactions of the chemical modification of chymotrypsin. I

  • M. M. Botvinik
  • G. N. Novodarova
  • V. L. D'yakov


The reaction of chymotrypsin with a number of modified analogs of the specific inhibitor of chymotrypsin L-1-tosylamido-2-phenylethyl chloromethyl ketone has been studied; the compounds investigated were D-1-tosylamido-2-phenethyl chloromethyl ketone, phenethyl chloromethyl ketone (PECK), and benzylethyl chloromethyl ketone (BCMK).

It has been shown that D-TAPK does not possess inhibiting activity. The inhibiting capacity of PECK is considerably reduced. A simultaneous decrease in the length of the carbon chain and the elimination of the tosylamino group (BCMK) practically destroys the inhibiting action of the reagent.


Aromatic Amino Acid Amino Acid Analysis Chymotrypsin Molar Excess Phenethyl 


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Copyright information

© The Faraday Press, Inc. 1969

Authors and Affiliations

  • M. M. Botvinik
    • 1
  • G. N. Novodarova
    • 1
  • V. L. D'yakov
    • 1
  1. 1.Moscow State UniversityUSSR

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