A study of the reactions of the chemical modification of chymotrypsin. I
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The reaction of chymotrypsin with a number of modified analogs of the specific inhibitor of chymotrypsin L-1-tosylamido-2-phenylethyl chloromethyl ketone has been studied; the compounds investigated were D-1-tosylamido-2-phenethyl chloromethyl ketone, phenethyl chloromethyl ketone (PECK), and benzylethyl chloromethyl ketone (BCMK).
It has been shown that D-TAPK does not possess inhibiting activity. The inhibiting capacity of PECK is considerably reduced. A simultaneous decrease in the length of the carbon chain and the elimination of the tosylamino group (BCMK) practically destroys the inhibiting action of the reagent.
KeywordsAromatic Amino Acid Amino Acid Analysis Chymotrypsin Molar Excess Phenethyl
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