Chemistry of Natural Compounds

, Volume 7, Issue 6, pp 781–783 | Cite as

Some aspects of the structure of the active center of the enzyme L-asparaginase

  • R. A. Zhagat
  • I. A. Vina
  • R. K. Bluma
  • R. F. Platnietse


Some functional groups in the active center of the enzyme L-asparaginase have been studied by the method of inhibitory analysis.

It has been established that the phenol groups of the tyrosine residues and the imidazole groups of the histidine residues are important for the occurrence of the enzymatic reaction.

The blocking of sulfhydryl, disulfide, and amino groups, and also of the hydroxy groups of serine and threonine, does not suppress the enzymatic activity.


Methylene Blue Asparaginase Succinic Anhydride Thioglycolic Acid Sulfanilic Acid 


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Literature cited

  1. 1.
    H. Marguardt, Arzn. Forsch.,18, 1380 (1968).Google Scholar
  2. 2.
    R. H. Adamson and S. Farbo, Cancer Chem. Report,52, 617 (1968).Google Scholar
  3. 3.
    H. M. Suld and R. A. Herbert, J. Biol. Chem.,245, 2802 (1970).PubMedGoogle Scholar
  4. 4.
    Y. S. Halpern and N. Grossowiez, Biochem. J.,65, 716 (1957).CrossRefGoogle Scholar
  5. 5.
    N. De Groot and N. Lichtenstein, Biochim. Biophys. Acta,40, 92, 99 (1960).CrossRefGoogle Scholar
  6. 6.
    D. B. Tower, E. L. Peters, and W. C. Curtis, J. Biol. Chem.,238, 983 (1963).PubMedGoogle Scholar
  7. 7.
    R. A. Zhagat, M. Ya. Orovere, I. K. Shprunka, I. A. Éidus, and G. Ya. Melle, Mikrobiol. (1971).Google Scholar
  8. 8.
    J. Roberts, G. Burson, and J. H. Hill, J. Bacteriol.,95, 2117 (1968).PubMedPubMedCentralGoogle Scholar
  9. 9.
    S. Moore and W. H. Stein, Methods in Enzymology, Vol. 6, Academic Press, New York (1963), p. 819.Google Scholar
  10. 10.
    J. R. Spies and D. C. Chambers, Analyt. Chem.,21, 1249 (1949).CrossRefGoogle Scholar
  11. 11.
    R. A. Zhagat, I. A. Vina, R. K. Bluma, and D. É. Barone, Biokhimiya (1971).Google Scholar
  12. 12.
    H. A. Whelan and J. C. Wriston, Biochemistry,8, 2386 (1969).CrossRefGoogle Scholar
  13. 13.
    T. O. Yellin and J. C. Wriston, Biochemistry,5, 1605 (1966).CrossRefGoogle Scholar
  14. 14.
    M. L. Anson, Advan. Protein Chem.,2, 361 (1944).CrossRefGoogle Scholar
  15. 15.
    P. Wagner, E. Irson, A. Arens, and K. Bauer, Biochem. Biophys. Res. Comm.,37, 383 (1969).CrossRefGoogle Scholar

Copyright information

© Consultants Bureau 1974

Authors and Affiliations

  • R. A. Zhagat
  • I. A. Vina
  • R. K. Bluma
  • R. F. Platnietse

There are no affiliations available

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