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Chemistry of Natural Compounds

, Volume 4, Issue 1, pp 25–26 | Cite as

A comparative evaluation of methods for determining the activity of chymotrypsin

  • M. T. Proskuryakov
Article
  • 28 Downloads

Summary

A method has been developed for determining the activity of chymotrypsin with respect to the coagulation of milk-acetate mixture in N. P. Pyatnitskii's modification. In specificity and sensitivity, this method approaches that based on the hydrolysis of N-acetyl-D, L-phenylalanine p-nitroanilide.

Keywords

Aromatic Amino Acid Chymotrypsin Nitroaniline Alanyl Acetate Mixture 

References

  1. 1.
    P. G. Storozhuk, Voprosy kurortologii, fizioterapii i lech. fizkul'tury, no. 6, 498–503, 1961.Google Scholar
  2. 2.
    N. P. Pyatnitskii and B. S. Mikhalenko, Izv. vyssh. uch. zaved., Pishchevaya tekhnol., no. 4, 147–149, 1965.Google Scholar
  3. 3.
    J. Tang, S. Wolf, R. Caputto, and R. Trucco, J. Biol. Chem., 234, no. 5, 1174–1178, 1959.PubMedGoogle Scholar
  4. 4.
    M. Seijffers, H. Segal, and L. Miller, Amer. J. Physiol., 207, no. 1, 8–12, 1964.PubMedGoogle Scholar
  5. 5.
    H. Tuppy, U. Wiesbauer, and E. Wintersberger, Hoppe Seyl. Z., 329, no. 3–6, 278–288, 1962.CrossRefGoogle Scholar
  6. 6.
    R. A. Matveeva, Ya. I. Lapuk, and V. M. Stepanov, Izv. AN SSSR, ser. khim., no. 3, 213, 1964.Google Scholar
  7. 7.
    J. H. Northrop, M. Kunitz, and R. M. Herriott, Crystalline Enzymes, 2nd ed., 262, Columbia University Press, New York, 1948.Google Scholar
  8. 8.
    N. P. Pyatnitskii and M. N. Selyukova, Voprosy pitaniya, no. 2, 176, 1960.Google Scholar
  9. 9.
    N. G. Belen'kii and L. B. Polonskaya, Alpha-Chymotrypsin [in Russian], 48, Moscow, 1962.Google Scholar

Copyright information

© The Faraday Press, Inc 1969

Authors and Affiliations

  • M. T. Proskuryakov
    • 1
  1. 1.Kuban Medical InstituteUSSR

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