Chemistry of Natural Compounds

, Volume 2, Issue 2, pp 105–108 | Cite as

A study of enzyme-substrate compounds

II. Preparation of acyl-L-valyl, D-valyl, and D-phenylalanyl derivatives of chymotrypsin
  • M. M. Botvinik
  • I. P. Kuranova
  • L. L. Ivanov
Article
  • 23 Downloads

Summary

1. From the reaction of chymotrypsin with the NPE of CBZ-L- and CBZ-D-valine the enzyme-substrate compounds CBZ-L-valylchymotrypsin and CBZ-D-valylchymotrypsin have been isolated. The methyl esters of benzoyl-D-phenylalanine and of benzoyl-L-valine also form enzyme-substrate compounds with chymotrypsin, although to a smaller extent. The methyl ester of benzoyl-D-valine does not react with chymotrypsin.

2. Conditions for the isolation of a water-soluble enzyme-substrate compound have been found.

Keywords

Methyl Ester Benzoyl Chymotrypsin Sodium Hydrogen Carbonate Phenyl Ester 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    A. K. Balls and H. N. Wood, J. Biol. Chem., 219, 245, 1956.PubMedGoogle Scholar
  2. 2.
    H. Gutfreund and B. R. Hammond, J. Biochem., 73, 526, 1959.CrossRefGoogle Scholar
  3. 3.
    G. R. Schonbaum, K. Nakamura, and M. L. Bender, J. Am. Chem. Soc., 81, 4746, 1959.CrossRefGoogle Scholar
  4. 4.
    F. J. Kezdy and M. L. Bender, J. Am. Chem. Soc., 36, 937, 1964.CrossRefGoogle Scholar
  5. 5.
    M M. Botvinik and I. P. Kuranova, DNA SSSR, 143, 1094, 1962.Google Scholar
  6. 6.
    G. Hein and C. Niemann, Proc. Nat. Acad. Sci., U.S., 47, 1341, 1961.CrossRefGoogle Scholar
  7. 7.
    M. Brenner and W. Huber, Helv. Chim. Acta., 36, 1109, 1953.CrossRefGoogle Scholar
  8. 8.
    R. L. M. Synge, J. Biochem., 42, 99, 1942.CrossRefGoogle Scholar
  9. 9.
    E. L. Smith, D. H. Spackman, and W. J. Polglase, J. Biol. Chem., 199, 801, 1952.PubMedGoogle Scholar
  10. 10.
    B. F. Erlanger, H. Sachs, and E. Brand, J. Am. Chem. Soc., 76, 1806, 1954.CrossRefGoogle Scholar
  11. 11.
    J. W. Hinman, E. L. Caron, and H. N. Christensen, J. Am. Chem. Soc., 72, 1620, 1960.CrossRefGoogle Scholar
  12. 12.
    T. Applewhite, H. Walt, and C. Niemann, J. Am. Chem. Soc., 80, 1465, 1958.CrossRefGoogle Scholar
  13. 13.
    H. Reihen and L. Knöpfe, Lieb. Ann., 523, 199, 1936.CrossRefGoogle Scholar
  14. 14.
    M. L. Bender and K. C. Kemp, J. Am. Chem. Soc., 79, 111, 1957.CrossRefGoogle Scholar
  15. 15.
    M. Bodansky and V. du Vigneaud, J. Am. Chem. Soc., 81, 5689, 1959.Google Scholar
  16. 16.
    M. M. Botvinik, et al., DAN SSSR, 156, 88, 1964.Google Scholar
  17. 17.
    B. Iselin, W. Rittel, P. Sicher, and R. Schwyzer, Helv. Chim. Acta., 40, 272, 1957.CrossRefGoogle Scholar
  18. 18.
    B. Zerner and M. L. Bender, J. Am. Chem. Soc., 86, 3669, 1964.CrossRefGoogle Scholar

Copyright information

© The Faraday Press, Inc 1967

Authors and Affiliations

  • M. M. Botvinik
    • 1
  • I. P. Kuranova
    • 1
  • L. L. Ivanov
    • 1
  1. 1.Lomonosov Moscow State UniversityUSSR

Personalised recommendations