Chemistry of Natural Compounds

, Volume 15, Issue 5, pp 624–626 | Cite as

Isolation and purification of biopolymers by affinity chromatography. IV. Preparation and some properties of trehalose immobilized on epoxy-activated sepharose [1]

  • B. A. Klyashchitskii


A description is given of the synthesis and some properties of a new affinity-adsorbent — “trehalose-epoxy-activated Sepharose 6B” (TES). The chromatographic behavior on TES of acid α-glucosidase (AG) and of concanavalin A (CA) has been studied. Washed epoxy-activated Sepharose 6B (1 g) was stirred at 40°C with a solution of 2.27 g of α,α′-trehalose in 3 ml of 0.1 N NaOH for 16 h. The gel was washed with 100 ml of distilled water and was stirred with 3 ml of 1 M ethanolamine at 20°C for 16 h and the TES obtained was washed with water. When an unpurified extract of human liver or a purified AG preparation was chromatographed in several buffer systems and water, no adsorption of the enzyme on the TES was observed. CA biospecifically binds to TES and can be eluted quantitatively with 0.1 M methyl α-D-glucoside or D-glucose. Some aspects of the affinity chromatography of biopolymers having an affinity for α-D-glucopyranosyl residues on TES are discussed.


Trehalose Caustic Soda Sodium Acetate Buffer Solution Nonreducing Glucose Glucopyranosyl Residue 


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Literature cited

  1. 1.
    E. M. Beier, B. A. Klyashchitskii, and G. Ya. Vidershain, Bioorgan. Khim.,5, 268 (1979).Google Scholar
  2. 2.
    L. Sundberg and J. Porath, J. Chromatogr.,90, 87 (1974).CrossRefGoogle Scholar
  3. 3.
    P. Vretblad, FEBS Lett.,47, 86 (1974); M. P. Silvanovich and R. D. Hill, Anal. Biochem.,73, 430 (1976); T. Alam and A. S. Balasubramanian, Biochim. Biophys. Acta,566, 327 (1979).CrossRefGoogle Scholar
  4. 4.
    R. Uy and F. Wold, Anal. Biochem.,81, 98 (1977).CrossRefGoogle Scholar
  5. 5.
    W. J. Gensler and I. Alam, J. Org. Chem.,42, 130 (1977).CrossRefGoogle Scholar
  6. 6.
    D. M. Belen'kii, D. B. Tsukerman, and E. L. Rozenfel'd, Biokhimiya,40, 927 (1975).Google Scholar
  7. 7.
    D. M. Belen'kii and E. L. Rozenfel'd, Biokhimiya,32, 1201 (1967).Google Scholar
  8. 8.
    T. Mega and Y. Matsushima, J. Biochem. (Tokyo),79, 185 (1976); T. Mega and Y. Matsushima, J. Biochem. (Tokyo),81, 571 (1977).CrossRefGoogle Scholar
  9. 9.
    D. M. Belen'kii and A. A. Kuznetsov, Biokhimiya,43, 1764 (1978).Google Scholar
  10. 10.
    I. J. Goldstein, C. E. Hollerman, and J. M. Merrick, Biochim. Biophys. Acta,97, 68 (1965).CrossRefGoogle Scholar
  11. 11.
    I. S. Lukomskaya and V. K. Gorodetskii, Biokhimiya,26, 477 (1961).Google Scholar

Copyright information

© Plenum Publishing Corporation 1980

Authors and Affiliations

  • B. A. Klyashchitskii

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