Chemistry of Natural Compounds

, Volume 15, Issue 6, pp 736–742 | Cite as

Identification and fractionation of the total histone of the cotton plant

  • G. F. Kasymov
  • N. I. Koryakina
  • T. P. Kopitsya
  • V. K. Burichenko


In order to isolate individual fractions of the histones of the cotton plant of variety 108-F and to investigate them further, the total histone has been chromatographed on Acrylex P-60 and Bio-Gel P-30. It has been shown that the most complete fractionation is achieved on a Acrylex P-60. By electrophoresis of the fractions in 15% PAAG in the presence of 6.25 M urea and on the basis of amino acid compositions, the following order of elution of the histones has been established: H1, H2B + H2A, H3, and H4. In a comparison of the amino acid composition of the H3 and H4 histones of the cotton plant with histones enriched with arginine from other species, a somewhat higher lysine : arginine ratio in these fractions has been found. On comparing the electrophoretic mobilities of the histone fractions from the cotton plant with the histones of calf thymus, some difference is observed which is apparently connected with a difference in their molecular weights.


Amino Acid Composition Electrophoretic Mobility Cotton Plant High Lysine Total Histone 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. 1.
    H. Hayashi and K. Iwai, J. Biochem.,72, 327 (1972).CrossRefGoogle Scholar
  2. 2.
    S. C. Rall, T. Okinaka, and C. F. Strniste, Biochemistry,16, No. 22, 4940 (1977).CrossRefGoogle Scholar
  3. 3.
    L. A. Bolund and E. W. Johns, Eur. J. Biochem.,35, 546 (1973).CrossRefGoogle Scholar
  4. 4.
    P. A. Temussi, J. Theor. Biol.,50, 25 (1975).CrossRefGoogle Scholar
  5. 5.
    E. W. Johns, Biochem. J.,92, No. 1, 55 (1964).CrossRefGoogle Scholar
  6. 6.
    E. W. Johns and D. M. P. Phillips, Biochem. J.,77, No. 3, 631 (1960).CrossRefGoogle Scholar
  7. 7.
    V. V. Sidorova, A. A. Yamaleeva, and V. G. Konarev, Abstracts of Lectures at the Fifth All-Union Symposium on the Structure and Function of the Cell Nucleus [in Russian], Novosibirsk (1975), p. 64.Google Scholar
  8. 8.
    V. V. Sidorova, T. P. Konitsya, G. F. Kasymova, N. I. Koryakina, V. K. Burichenko, and V. G. Konarev, Dokl. Akad. Nauk Tadz. SSR,21, No. 4, 31 (1978).Google Scholar
  9. 9.
    N. J. Jardine and J. L. Leaver, Biochem. J.,169, 103 (1978).CrossRefGoogle Scholar
  10. 10.
    B. Wakim, Arch. Biochem. Biophys.,176, 510 (1976).CrossRefGoogle Scholar
  11. 11.
    Yu. A. Tyrsin, I. A. Krasheninnikov, and E. G. Tyrsina, Biokhimiya,42, No. 5, 898 (1977).Google Scholar
  12. 12.
    M. Tazal and R. D. Cole, J. Biol. Chem.,252, No. 12, 4068 (1977).Google Scholar
  13. 13.
    S. Panyim and R. Chalkley, Arch. Biochem. Biophys.,130, 337 (1969).CrossRefGoogle Scholar
  14. 14.
    L. C. Hnilica, M. E. McClure, and T. C. Spelsberg, in: Histones and Nucleohistones, D. M. P. Phillips (ed.), Plenum, London (1971).Google Scholar
  15. 15.
    N. J. Jardine and J. L. Leaver, Biochem. J.,169, 103 (1978).CrossRefGoogle Scholar
  16. 16.
    P. Nadeau, D. Pallota, and J. C. Lafontaine, Can. J. Biochem.,55, No. 7, 721 (1977).CrossRefGoogle Scholar
  17. 17.
    M. Tazal and R. D. Cole, J. Biol. Chem.,252, No. 12, 4068 (1977).Google Scholar
  18. 18.
    U. K. Laemmli, Nature (London),227, 680 (1970).CrossRefGoogle Scholar
  19. 19.
    J. D. Barret and E. W. Johns, J. Chromatogr.,75, 161 (1973).CrossRefGoogle Scholar
  20. 20.
    V. G. Alfrey, V. G. Littay, and A. E. Mirsky, J. Cell. Biol.,21, 213 (1964).CrossRefGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1980

Authors and Affiliations

  • G. F. Kasymov
  • N. I. Koryakina
  • T. P. Kopitsya
  • V. K. Burichenko

There are no affiliations available

Personalised recommendations