Isolation of ribosomes from cotton seeds
1. It has been established that the method of precipitation with ethanol in the presence of an increased concentration of Mg2+ can be used for the rapid isolation of the ribosome fraction from plant material.
2. The optimum conditions for the complete precipitation of the ribosome fraction from a homogenate of cotton seeds have been selected.
3. By treating an extract of the seeds with 0.5 M KCl it was possible to reduce the amount of tRNA in a preparation of the total rRNA.
KeywordsRazor Blade Cotton Seed Ammonium Persulfate Leucite Rapid Isolation
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