Quantitative determination of glycerol in phospholipids by gas-liquid chromatography
1. A new method for the quantitative determination of glycerol entering into the composition of natural glycerophosphatides has been developed.
2. The method described has been used for the analysis of egg-yolk cephalin and lecithin and also the cephalin, lecithin, and cardiolipin of the cardiac muscle of large-horned cattle.
3. Using heart phospholipids as an example, the possibility has been shown of the separate determination of the glycerol in diacyl- and acylalkylidenephosphatides.
KeywordsLecithin Chloroform Layer Triacetin Alkaline Methanolysis Lecithin Fraction
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