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Chemistry of Natural Compounds

, Volume 14, Issue 4, pp 420–424 | Cite as

Supports for the solid-phase synthesis of oligonucleotides with a 5′-terminal phosphate group

  • Yu. V. Tumanov
  • V. K. Potapov
  • Z. A. Shabarova
Article
  • 19 Downloads

Summary

A simple method is proposed for obtaining two kinds of polymeric supports of the polystyrene type for the solid-phase synthesis of oligonucleotides containing 5′-terminal phosphate groups. For both kinds of supports the optimum conditions of adding the first nucleotide and of splitting out the nucleotide material from the polymer have been worked out and the synthesis of a number of di- and trinucleotides of the deoxy series has been performed.

Keywords

Benzidine Polymeric Support Sulfonyl Chloride Phosphorus Oxychloride Terminal Phosphate Group 

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Literature cited

  1. 1.
    M. J. Gait and R. C. Sheppard, Nucl. Acid Res., 1135 (1977).Google Scholar
  2. 2.
    H. Koster, A. Pollak, and F. Gramer, Ann. Chem., 946 (1974).Google Scholar
  3. 3.
    Yu. V. Tumanov, M. G. Shlyapnikov, V. K. Potapov, and Z. A. Shabarova, Bioorg. Khim.,1, 1291 (1975).Google Scholar
  4. 4.
    R. L. Letsinger and T. Shimidzu, Bull. Chem. Soc. Jpn.,44, 1673 (1971).CrossRefGoogle Scholar
  5. 5.
    K. F. Yip and K. C. Tsou, J. Am. Chem. Soc.,93, 3272 (1971).CrossRefGoogle Scholar
  6. 6.
    T. M. Chapman and D. G. Kleid, J. Chem. Soc. Chem. Commun.,5, 193 (1973).CrossRefGoogle Scholar
  7. 7.
    H. Koster and K. Heyns, Tetrahedron Lett.,16, 1531 (1972).CrossRefGoogle Scholar
  8. 8.
    S. I. Turkin, V. K. Potapov, Z. A. Shabarova, V. F. Zarytova, and D. G. Knorre, Bioorg. Khim.,1, 1430 (1975).Google Scholar
  9. 9.
    E. Ohtsuka, S. Morioka, and M. Ikehara, J. Am. Chem. Soc.,94, 3229 (1972).CrossRefGoogle Scholar
  10. 10.
    M. M. Kabachnik, N. G. Timofeeva, M. V. Budanov, V. K. Potapov, and Z. A. Shabarova, Zh. Obshch. Khim.,43, 379 (1973).Google Scholar
  11. 11.
    R. K. Ralph, W. J. Connors, H. Shaller, and H. G. Khorana, J. Am. Chem. Soc.,85, 1983 (1963).CrossRefGoogle Scholar
  12. 12.
    H. Shaller and H. G. Khorana, J. Am. Chem. Soc.,85, 3821 (1963).CrossRefGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1979

Authors and Affiliations

  • Yu. V. Tumanov
  • V. K. Potapov
  • Z. A. Shabarova

There are no affiliations available

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