Method of determining the position of guanosine residues in oligodeoxyribonucleotides
A method is proposed for determining the position of guanosine residues in oligo-deoxyribonucleotides. After limited modification of the oligonucleotide with glyoxal, a mixture of molecules of the initial oligonucleotide with different degrees of modification is formed. In the presence of borate ions, the glyoxal-modified guanosine residues form negatively charged borate complexes which inhibit the action of snake venom phosphodiesterase. The treatment of such a complex with the enzyme forms a mixture of fragments of the initial oligonucleotide the 5′-terminal sequences of which are identical while at the 3′-end there are modified guanosine residues. The determination of the length of these fragments provides the necessary information on the positions of the guanosine residues in the chain of the initial oligonucleotides. The positions of the guanosine residues in eight synthetic oligodeoxyribonucleotides have been confirmed with the aid of the method developed.
KeywordsBorate Buffer Glyoxal Snake Venom Boronic Acid Guanine Residue
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- 6.E. D. Sverdlov, G. S. Monastyrskaya, and É. I. Budovskii, Mol. Biol.,11, 116 (1977).Google Scholar
- 7.V. G. Korobko, S. A. Grachev, and N. A. Petrov, Bioorg. Khim.,3, 1423 (1977).Google Scholar
- 9.E. Yu. Krynetskii, V. G. Metelev, V. D. Smirnov, and Z. A. Shabarova, Khim. Prir. Soedin., 253 (1978).Google Scholar
- 10.E. A. Ivanova, I. I. Kolodkina, and A. M. Yurkevich, Zh. Obshch. Khim.,41, 455 (1971).Google Scholar
- 11.S. V. Kuz'min, in: D. G. Knorre and T. V. Venkstern (eds.), The Ultramicroanalysis of Nucleic Acids [in Russian], Moscow (1973), pp. 95–103.Google Scholar