Advertisement

Chemistry of Natural Compounds

, Volume 9, Issue 5, pp 551–564 | Cite as

Isoelectric focusing of proteins in gels

  • A. Ya. Strongin
  • E. D. Levin
  • V. M. Stepanov
Article
  • 36 Downloads

Summary

The method of isoelectric focusing in a gel has become widely used in spite of the fact that the first papers on this question were published only four years ago. This is due to the advantages of the method: high sensitivity and resolving power, simplicity and rapidity, and possibility of combination with other methods.

Keywords

Ammonium Persulfate Acid Proteinase Cyanogen Bromide FEBS Letter Science Tool 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. 1.
    H. Svensson, Acta Chem. Scand.,15, 325 (1961).CrossRefGoogle Scholar
  2. 2.
    H. Svensson, Acta Chem. Scand.,16, 456 (1962).CrossRefGoogle Scholar
  3. 3.
    O. Vesterberg and H. Svensson, Acta Chem. Scand.,20, 820 (1966).PubMedCrossRefGoogle Scholar
  4. 4.
    O. Vesterberg, Acta Chem. Scand.,23, 2653 (1969).CrossRefGoogle Scholar
  5. 5.
    H. Svensson, Arch. Biochem. and Biophys. Suppl.,1, 132 (1962).Google Scholar
  6. 6.
    R. Grasbeck, Acta Chem. Scand.,22, 1041 (1968).PubMedCrossRefGoogle Scholar
  7. 7.
    O. Vesterberg, Inaugural dissertation, Karolinska institutet, Sweden (1968).Google Scholar
  8. 8.
    H. Haglund, Science Tools,14, 17 (1967).Google Scholar
  9. 9.
    H. Haglund, in: Methods in Biochemical Analysis (ed. D. Glick), Interscience Publishers, New York (1969).Google Scholar
  10. 10.
    G. Dale and A. L. Latner, Lancet, 7547, 847 (1968–1).Google Scholar
  11. 11.
    C. W. Wrigley, Science Tools,15, 17 (1968).Google Scholar
  12. 12.
    C. W. Wrigley, J. Chromatogr.,36, 362 (1968).PubMedCrossRefGoogle Scholar
  13. 13.
    R. F. Riley and M. K. Coleman, J. Lab. Clin. Med.,72, 714 (1968).PubMedGoogle Scholar
  14. 14.
    J. S. Fawcett, FEBS Letters,1, 81 (1968).PubMedCrossRefGoogle Scholar
  15. 15.
    N. Catsimpoolas, Analyt. Biochem.,26, 480 (1968).PubMedCrossRefGoogle Scholar
  16. 16.
    H. Maurer, Disk-Elektrophorese, Theorie und Praxis der diskontinuierlichen Polyakrylamidgel-Elektrophorese, Walter de Gruyter, Berlin (1968).Google Scholar
  17. 17.
    N. Catsimpoolas and E. Leuthner, Biochim. et Biophys. Acta,181, 404 (1969).CrossRefGoogle Scholar
  18. 18.
    R. Finlayson and A. Chrambach, Analyt. Biochem.,40, 296 (1971).CrossRefGoogle Scholar
  19. 19.
    J. W. Drysdale, P. G. Righetti, and H. F. Bunn, Biochim. et Biophys. Acta,229, 42 (1971).CrossRefGoogle Scholar
  20. 20.
    C. W. Wrigley, Biochem. Genetics,4, 509 (1970).CrossRefGoogle Scholar
  21. 21.
    J. G. Tasseron, H. Diringer, N. Frohwirth, S. S. Mirvish, and C. Heidelberger, Biochemistry,9, 1636 (1970).PubMedCrossRefGoogle Scholar
  22. 22.
    L. H. Percival, G. G. Dunckley, and H. D. Purves, Aust. J. Exper. Biol. and Med. Sci.,48, 171 (1970).CrossRefGoogle Scholar
  23. 23.
    U. J. Lewis, E. V. Cheever, and W. C. Hopkins, Biochim. et Biophys. Acta,214, 498 (1970).CrossRefGoogle Scholar
  24. 24.
    P. Righetti and J. W. Drysdale, Biochim. Biophys. Acta,236, 17 (1971).PubMedCrossRefGoogle Scholar
  25. 25.
    J. M. Brewer, Science,156, 256 (1967).PubMedCrossRefGoogle Scholar
  26. 26.
    W. M. Mitchell, Biochim. et Biophys. Acta,147, 171 (1967).CrossRefGoogle Scholar
  27. 27.
    L. J. Kaplan and J. F. Foster, Biochemistry,10, 631 (1971).CrossRefGoogle Scholar
  28. 28.
    O. Vesterberg, Biochim. et Biophys. Acta,257, 11 (1972).CrossRefGoogle Scholar
  29. 29.
    M. Gronow and G. Griffiths, FEBS Letters,15, 340 (1971).PubMedCrossRefGoogle Scholar
  30. 30.
    A. Scanu, J. Toth, C. Edelstein, S. Koda, and E. Stiller, Biochemistry,8, 3309 (1969).PubMedCrossRefGoogle Scholar
  31. 31.
    H. G. Rittenhouse, J. Heptinstall, and B. A. McFadden, Biochemistry,10, 4045 (1971).PubMedCrossRefGoogle Scholar
  32. 32.
    K. G. Kenrick and J. Margolis, Analyt. Biochem.,33, 205 (1970).CrossRefGoogle Scholar
  33. 33.
    J. R. Florini, R. P. Brivio, and B-A. M. Battelle, Analyt. Biochem.,40, 345 (1971).PubMedCrossRefGoogle Scholar
  34. 34.
    A. C. Peacock and C. W. Dingman, Biochemistry,7, 668 (1968).PubMedCrossRefGoogle Scholar
  35. 35.
    N. Catsimpoolas, Science Tools,16, 1 (1969).Google Scholar
  36. 36.
    N. Catsimpoolas, Separation Science,5, 523 (1970).CrossRefGoogle Scholar
  37. 37.
    N. Catsimpoolas and J. Wang, Analyt. Biochem.,39, 141 (1971).PubMedCrossRefGoogle Scholar
  38. 38.
    N. Catsimpoolas, Clin. Chim. Acta,23, 237 (1968).CrossRefGoogle Scholar
  39. 39.
    N. Catsimpoolas, Biochim. et Biophys. Acta,175, 214 (1969).CrossRefGoogle Scholar
  40. 40.
    N. Catsimpoolas, Immunochemistry,6, 501 (1969).PubMedCrossRefGoogle Scholar
  41. 41.
    M. B. Hayes and D. Wellner, J. Biol. Chem.,244, 6636 (1969).PubMedGoogle Scholar
  42. 42.
    S. Lewin, Biochem. J.,117, 41P (1970).Google Scholar
  43. 43.
    E. Haber, Federat. Proc.,29, 66 (1970).Google Scholar
  44. 44.
    T. P. Fondy, K. J. Herwig, S. J. Sollohub, and D. B. Rutherford, Arch. Biochem. and Biophys.,145, 583 (1971).CrossRefGoogle Scholar
  45. 45.
    C. R. Ross, S. Curry, A. W. Schwartz, and T. P. Fondy, Arch. Biochem. and Biophys.,145, 591 (1971).CrossRefGoogle Scholar
  46. 46.
    T. P. Fondy, J. Solomon, and C. R. Ross, Arch. Biochem. and Biophys.,145, 604 (1971).CrossRefGoogle Scholar
  47. 47.
    B. Lerch and G. Wolf, Biochim. et Biophys. Acta,258, 206 (1972).CrossRefGoogle Scholar
  48. 48.
    E. M. Spencer and T. P. King, J. Biol. Chem.,246, 201 (1971).PubMedGoogle Scholar
  49. 49.
    M. P. Salaman and A. R. Williamson, Biochem. J.,122, 93 (1971).PubMedPubMedCentralCrossRefGoogle Scholar
  50. 50.
    R. V. Josephson, S. K. Maheswaran, C. W. Morr, R. Jenness, and R. K. Lindorfer, Analyt. Biochem.,40, 476 (1971).PubMedCrossRefGoogle Scholar
  51. 51.
    P. Doerr and A. Chrambach, Analyt. Biochem.,42, 96 (1971).PubMedCrossRefGoogle Scholar
  52. 52.
    H. Delincee and B. J. Radola, Intern. J. Radiat. Biol.,19, 93 (1971).Google Scholar
  53. 53.
    A. Conway-Jacobs and L. W. Lewin, Analyt. Biochem.,43, 394 (1971).PubMedCrossRefGoogle Scholar
  54. 54.
    P. J. Bechtel, A. M. Pearson, and C. E. Bodwell, Analyt. Biochem.,43, 509 (1971).PubMedCrossRefGoogle Scholar
  55. 55.
    R. E. Jones, H. A. Hemmings, and W. P. Faulk, Immunochemistry,8, 299 (1971).PubMedCrossRefGoogle Scholar
  56. 56.
    A. D. Friesen, J. C. Jamieson, and F. E. Ashton, Analyt. Biochem.,41, 149 (1971).PubMedCrossRefGoogle Scholar
  57. 57.
    R. E. MacKenzie and J. C. Rabinowitz, J. Biol. Chem.,246, 3731 (1971).PubMedGoogle Scholar
  58. 58.
    D. Bobb and B. H. J. Hofstee, Analyt. Biochem.,40, 209 (1971).PubMedCrossRefGoogle Scholar
  59. 59.
    D. Onica, Revue Roumaine de Biochimie,7, 287 (1970).Google Scholar
  60. 60.
    K. C. Humphryes, J. Chromatogr.,49, 503 (1970).PubMedCrossRefGoogle Scholar
  61. 61.
    A. Chrambach, R. A. Reisfeld, M. Wyckoff, and J. Zaccari, Analyt. Biochem.,20, 150 (1967).PubMedCrossRefGoogle Scholar
  62. 62.
    Z. L. Awdeh, Science Tools,16, 42 (1969).Google Scholar
  63. 63.
    O. Vesterberg. Biochim. et Biophys. Acta,243, 345 (1971).CrossRefGoogle Scholar
  64. 64.
    R. Frater, J. Chromatogr.,50, 469 (1970).CrossRefGoogle Scholar
  65. 65.
    S. Ward, Analyt. Biochem.,33, 259 (1970).PubMedCrossRefGoogle Scholar
  66. 66.
    J. Lamy, J. Lamy, S. Compin, and J. Weill, Ann. Biol. Clin.,29, 125 (1971).Google Scholar
  67. 67.
    D. Wellner, Analyt. Chemistry,43, A59 (1971).Google Scholar
  68. 68.
    N. Catsimpoolas, Arch. Biochem. and Biophys.,131, 185 (1969).CrossRefGoogle Scholar
  69. 69.
    N. Chamoles and D. Karcher, Clin. Chim. Acta,30, 359 (1970).PubMedCrossRefGoogle Scholar
  70. 70.
    W. J. Kim and T. T. White, Biochim. et Biophys. Acta,242, 441 (1971).CrossRefGoogle Scholar
  71. 71.
    M. Fosset, L. W. Muir, L. D. Nielson, and E. H. Fischer, Biochemistry,10, 4105 (1971).PubMedCrossRefGoogle Scholar
  72. 72.
    J. W. Drysdale, Biochim. et Biophys. Acta,207, 256 (1970).CrossRefGoogle Scholar
  73. 73.
    A. Fernandez-Sorensen and M. Don Carlson, J. Biol. Chem.,246, 3485 (1971).PubMedGoogle Scholar
  74. 74.
    J. Spragg and K. F. Austen, J. of Immunology,107, 1512 (1971).Google Scholar
  75. 75.
    Z. L. Awdeh, A. R. Williamson, and B. A. Askonas, Biochem. J.,116, 241 (1970).PubMedPubMedCentralCrossRefGoogle Scholar
  76. 76.
    R. Frater, Analyt. Biochem.,38, 536 (1971).CrossRefGoogle Scholar
  77. 77.
    D. H. Leaback and A. C. Rutter, Biochem. and Biophys. Res. Communs.,32, 447 (1968).CrossRefGoogle Scholar
  78. 78.
    Z. L. Awdeh, A. R. Williamson, and B. A. Askonas, Natura,219, 66 (1968).CrossRefGoogle Scholar
  79. 79.
    J. Sonnet and J. P. De Noyette, Science Tools,18, 12 (1971).Google Scholar
  80. 80.
    B. J. Radola, Biochim. et Biophys. Acta,194, 335 (1969).CrossRefGoogle Scholar
  81. 81.
    B. J. Radola, J. Chromatogr.,38, 61 (1968).PubMedCrossRefGoogle Scholar
  82. 82.
    G. Dale and A. L. Latner, Clin. Chim. Acta,24, 61 (1969).PubMedCrossRefGoogle Scholar
  83. 83.
    G. Dale, A. L. Latner, and T. J. Muckle, J. Clinic. Pathology,23, 35 (1970).CrossRefGoogle Scholar
  84. 84.
    V. Macko and H. Stegemann, Hoppe-Seyler's Z. Physiol. Chem.,50, 917 (1969).Google Scholar
  85. 85.
    N. Catsimpoolas, Clin. Chim. Acta,27, 222 (1970).CrossRefGoogle Scholar
  86. 86.
    P. J. Gaffney, Nature,230, 54, March 10 (1970).Google Scholar
  87. 87.
    Z. L. Awdeh, A. R. Williamson, and B. A. Askonas, FEBS Letters,5, 275 (1969).PubMedCrossRefGoogle Scholar
  88. 88.
    M. A. Geidan, M. R. Buka, and R. A. Zhagat, Khim. Prirodn. Soedin., 98 (1971).Google Scholar
  89. 89.
    N. M. Azarenkova, Conference of Young Scientists of the All-Union Scientific-Research Institute for the Genetics and Breeding of Industrial Microorganisms [in Russian], Moscow (1972), p. 3.Google Scholar
  90. 90.
    W. Rucker and B. J. Radola, Planta,99, 192 (1971).PubMedCrossRefGoogle Scholar
  91. 91.
    P. J. Bechtel, A. M. Pearson, and C. E. Bodwell, Federat. Proc.,30, 1504 (1971).Google Scholar
  92. 92.
    J. W. Drysdale and P. G. Righetti, Federat. Proc.,29, 3557 (1970).Google Scholar
  93. 93.
    J. H. Pincus, J. C. Jaton, K. J. Bloch, and E. Haber, J. Immunology,104, 1149 (1970).Google Scholar
  94. 94.
    E. Maron, C. Shiozawa, R. Arnon, and M. Sela, Biochemistry,10, 763 (1971).PubMedCrossRefGoogle Scholar
  95. 95.
    G. J. O'Neill and D. A. L. Davies, Biochim. et Biophys. Acta,243, 337 (1971).CrossRefGoogle Scholar
  96. 96.
    P. K. Shrivastava, H. Goch, and K. Zakrzawski, Biochim. et Biophys. Acta,271, 310 (1972).CrossRefGoogle Scholar
  97. 97.
    P. Delmotte, Z. Klin. Chem. und Klin. Biochem.,9, 334 (1971).Google Scholar
  98. 98.
    C. F. Shannon and J. M. Hill, Biochemistry,10, 3021 (1971).PubMedCrossRefGoogle Scholar
  99. 99.
    J. A. Beeley, Archs. Oral Biol.,14, 559 (1969).CrossRefGoogle Scholar
  100. 100.
    N. Catsimpoolas and E. W. Meyer, Arch. Biochem. and Biophys.,132, 279 (1969).CrossRefGoogle Scholar
  101. 101.
    I. Koshiyama, Int. J. Peptide Protein Res.,4, 167 (1972).CrossRefGoogle Scholar
  102. 102.
    C. M. Park, J. Biol. Chem.,245, 5390 (1970).PubMedGoogle Scholar
  103. 103.
    N. Catsimpoolas, Separation Science,4, 483 (1969).CrossRefGoogle Scholar
  104. 104.
    S. Lewin, Postgrad. Med. J.,45, 729 (1969).PubMedPubMedCentralCrossRefGoogle Scholar
  105. 105.
    A. J. Barrett, Biochem. J.,117, 601 (1970).PubMedPubMedCentralCrossRefGoogle Scholar
  106. 106.
    G. von Kostner, W. Albert, and A. Holasek, Hoppe-Seyler's Z. Physiol. Chem.,350, 347 (1969).Google Scholar
  107. 107.
    J. Schultz and K. Mosbach, Eur. J. Biochem.,22, 153 (1971).PubMedCrossRefGoogle Scholar
  108. 108.
    K. Yagi and N. Ohishi, J. Biochem.,71, 993 (1972).PubMedCrossRefGoogle Scholar
  109. 109.
    V. Pliska, J. F. McKelvy, and H. Sachs, Federat. Proc.,30, 821 (1971).Google Scholar
  110. 110.
    V. Pliska, J. F. McKelvy, and H. Sachs, Eur. J. Biochem.,28, 110 (1972).PubMedCrossRefGoogle Scholar
  111. 111.
    M. R. Davies and B. M. Dean, FEBS Letters,18, 283 (1971).PubMedCrossRefGoogle Scholar
  112. 112.
    E. Alpert, J. W. Drysdale, K. J. Isselbacher, and P. H. Schur, J. Biol. Chem.,247, 3792 (1972).PubMedGoogle Scholar
  113. 113.
    D. M. Payne, D. W. Porter, and R. W. Gracy, Arch. Biochem. and Biophys.,151, 122 (1972).CrossRefGoogle Scholar
  114. 114.
    S. Krantz, M. Lober, and H. Fiedler, FEBS Letters,11, 100 (1970).PubMedCrossRefGoogle Scholar
  115. 115.
    I. Smith, P. J. Lightstone, and J. D. Perry, Clin. Chim. Acta,35, 59 (1971).PubMedCrossRefGoogle Scholar
  116. 116.
    P. C. Montgomery and A. R. Williamson, Natura,228, 1306 (1970).CrossRefGoogle Scholar
  117. 117.
    H. F. Bunn and J. W. Drysdale, Biochim. et Biophys. Acta,229, 51 (1971).CrossRefGoogle Scholar
  118. 118.
    J. Bours and W. J. Van Doorenmaalen, Science Tools,17, 36 (1970).Google Scholar
  119. 119.
    J. G. Beeley and E. McCairns, Biochim. et Biophys. Acta,271, 204 (1972).CrossRefGoogle Scholar
  120. 120.
    A. B. McQuade and W. G. Crewthner, Biochim. et Biophys. Acta,191, 762 (1969).CrossRefGoogle Scholar
  121. 121.
    J. C. Bagshaw and J. W. Drysdale, Federat. Proc.,30, 1454 (1971).Google Scholar
  122. 122.
    H. Delincee and B. J. Radola, Biochim. et Biophys. Acta,200, 404 (1970).CrossRefGoogle Scholar
  123. 123.
    H. Delincee and B. J. Radola, Biochim. et Biophys. Acta,214, 178 (1970).CrossRefGoogle Scholar
  124. 124.
    J. G. Beeley, Biochim. et Biophys. Acta,230, 593 (1971).Google Scholar

Copyright information

© Plenum Publishing Corporation 1975

Authors and Affiliations

  • A. Ya. Strongin
  • E. D. Levin
  • V. M. Stepanov

There are no affiliations available

Personalised recommendations