Conclusions
The reaction of yeast inorganic pyrophosphatase with an excess of maleic anhydride at 2° C for 5 min leads to the acylation of all ε-amino groups of lysine residues.
The protective groups are completely removed at pH 3.5 for 16 hr at 60° C. Acylation with maleic anhydride is accompanied by the dissociation of the yeast inorganic pyrophosphatase into its subunits.
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Khimiya Prirodnykh Soedinenii, Vol. 6, No. 1, pp. 123–127, 1970
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Avaeva, S.M., Beisembaeva, R.U. & Lebedeva, Z.I. Reaction of yeast inorganic pyrophosphatase with maleic anhydride. Chem Nat Compd 6, 115–118 (1970). https://doi.org/10.1007/BF00564174
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DOI: https://doi.org/10.1007/BF00564174