Advertisement

Chemistry of Natural Compounds

, Volume 6, Issue 1, pp 115–118 | Cite as

Reaction of yeast inorganic pyrophosphatase with maleic anhydride

  • S. M. Avaeva
  • R. U. Beisembaeva
  • Z. I. Lebedeva
Article
  • 32 Downloads

Conclusions

The reaction of yeast inorganic pyrophosphatase with an excess of maleic anhydride at 2° C for 5 min leads to the acylation of all ε-amino groups of lysine residues.

The protective groups are completely removed at pH 3.5 for 16 hr at 60° C. Acylation with maleic anhydride is accompanied by the dissociation of the yeast inorganic pyrophosphatase into its subunits.

Keywords

Maleic Anhydride Protective Group Succinic Anhydride Sedimentation Coefficient Performic Acid 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    R. F. Goldberger and C. B. Anfinsen, Biochem., 1, no. 3, 401–405, 1962.CrossRefGoogle Scholar
  2. 2.
    C. H. Li, J. S. Dixon, and D. Shung, J. Am. Chem. Soc., 80, 2587, 1958.CrossRefGoogle Scholar
  3. 3.
    A. F. S. A. Habeeb, H. G. Cassidy, and S. J. Singer, Biochem. Biophys. Acta, 29, 587, 1958.CrossRefGoogle Scholar
  4. 4.
    C. H. Li and J. Bertch, J. Biol. Chem., 235, 2638, 1960.PubMedGoogle Scholar
  5. 5.
    P. I. Butler, J. I. Harris, B. S. Hartley, and R. Leberman, Biochem. J., 103, no. 3, 78, 1967.PubMedCentralGoogle Scholar
  6. 6.
    M. H. Freedman, A. L. Grossberg, and D. Pressman, Biochem., 7, 1941, 1968.CrossRefGoogle Scholar
  7. 7.
    J. I. Harris and R. N. Perham, Nature, 219, 1025, 1968.CrossRefGoogle Scholar
  8. 8.
    M. Kunitz, Arch. Biochem. Biophys., 92, 270, 1961.CrossRefGoogle Scholar
  9. 9.
    A. C. Buton, N. A. Fuller, and S. G. Perry, J. Chem. Soc., no. 5, 2918, 1967.Google Scholar
  10. 10.
    J. Bailey, Methods of Protein Chemistry [Russian translation], 138, 1965.Google Scholar
  11. 11.
    S. M. Avaeva, E. A. Braga, and A. M. Egorov, Biofizika, 1143, 1968.Google Scholar

Copyright information

© Consultants Bureau 1972

Authors and Affiliations

  • S. M. Avaeva
    • 1
  • R. U. Beisembaeva
    • 1
  • Z. I. Lebedeva
    • 1
  1. 1.Moscow State UniversityUSSR

Personalised recommendations