20α-Hydroxysteroid oxidoreductase fromActinomyces roseochromogenus ATCC 3347. Industion, primary purification, substrate specificity
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The formation of 20α-hydroxysteroid oxidoreductase with a constitutive enzyme ofActinomyces roseochromogenus is activated by certain predominantly monohydroxy derivatives of pregnane. Some steroids suppress the formation of the enzyme. Intact cells ofA. roseochromogenus and a cell-free enzyme system possess identical substrate specificities which are close to the substrate specificity of the 20α-hydroxysteroid oxidoreductase of rat testes and reduce only 20-oxopregnanes with a 17-oxygen function. The specific role of the 17-oxogen function appears at the substrate-enzyme level.
KeywordsNADP Ammonium Sulfate Pregnane Hydroxyprogesterone Constitutive Enzyme
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