Cleavage of the peptide chain of blood-group substances at the hydroxyamino acid links
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1. A method for the cleavage of the peptide chain of glycoproteins containing O-glycosidic bonds of hydroxyamino acids based on the introduction into the peptide chain of reactive enamine groupings through the β-elimination of the carbohydrate moieties has been proposed for the first time.
2. The degradation based on this principle of the peptide chain of blood group substances A + H has been effected. Various conditions for this degradation and also the dependence of the degree of degradation on the conditions of β-elimination have been studied.
3. The basic structural nature of the molecule of blood group substance A + H has been deduced. The skeleton of the molecule is a long polypeptide chain.
KeywordsBromine Peptide Chain Caustic Soda Peptide Fraction Mild Acid Hydrolysis
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