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Quantitative determination of phosphocholine and the activity of phospholipase C

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Chemistry of Natural Compounds Aims and scope

Summary

1. A quantitative method for determining phosphocholine by means of thin-layer chromatography has been proposed.

2. In the enzymatic cleavage of lecithins with phospholipase C, this method permits the amount of diglycerides formed to be determined as well as the phosphocholine.

3. The agreement of the results of the analysis of phosphocholine and the diglycerides is a reliable criterion of the activity of the phospholipase C.

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Authors and Affiliations

  1. Institute of the Chemistry of Natural Compounds, AS USSR, USSR

    E. V. Dyatlovitskaya, V. I. Volkova, M. V. Ispolatovskaya & L. D. Bergel'son

  2. Gamalei Institute of Epidemiology and Microbiology, AMS USSR, USSR

    E. V. Dyatlovitskaya, V. I. Volkova, M. V. Ispolatovskaya & L. D. Bergel'son

Authors
  1. E. V. Dyatlovitskaya
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  2. V. I. Volkova
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  3. M. V. Ispolatovskaya
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  4. L. D. Bergel'son
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Additional information

Khimiya Prirodnykh Soedinenii, Vol. 2, No. 4, pp. 233–235, 1966

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Dyatlovitskaya, E.V., Volkova, V.I., Ispolatovskaya, M.V. et al. Quantitative determination of phosphocholine and the activity of phospholipase C. Chem Nat Compd 2, 188–189 (1966). https://doi.org/10.1007/BF00564085

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  • DOI: https://doi.org/10.1007/BF00564085

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