Quantitative determination of phosphocholine and the activity of phospholipase C
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1. A quantitative method for determining phosphocholine by means of thin-layer chromatography has been proposed.
2. In the enzymatic cleavage of lecithins with phospholipase C, this method permits the amount of diglycerides formed to be determined as well as the phosphocholine.
3. The agreement of the results of the analysis of phosphocholine and the diglycerides is a reliable criterion of the activity of the phospholipase C.