Chemistry of Natural Compounds

, Volume 10, Issue 2, pp 204–206 | Cite as

Quantitative determination of amino acids in protein hydrolyzates by thin-layer chromatography

  • M. D. Lutsik
  • I. I. Litvin
  • V. A. Monastyrskii
  • Ya. I. Aleksevich


A micro method for the quantitative determination of amino acids in protein hydrolyzates has been proposed which permits the analysis of two hydrolyzates to be performed in 4–5 h. After the separation of the amino acids by TLC in cellulose, they are determined quantitatively by the colorimetric method using a MKMF-1 microphotocolorimeter. Proline and tryptophan are determined separately with the aid of specific test-tube reactions. The sensitivity of the method permits the analysis of 10–15 µg of aminoacid mixture.


Proline Ninhydrin Protein Hydrolyzate Isatin Amino Nitrogen 


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Copyright information

© Plenum Publishing Corporation 1975

Authors and Affiliations

  • M. D. Lutsik
  • I. I. Litvin
  • V. A. Monastyrskii
  • Ya. I. Aleksevich

There are no affiliations available

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