Quantitative determination of amino acids in protein hydrolyzates by thin-layer chromatography
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A micro method for the quantitative determination of amino acids in protein hydrolyzates has been proposed which permits the analysis of two hydrolyzates to be performed in 4–5 h. After the separation of the amino acids by TLC in cellulose, they are determined quantitatively by the colorimetric method using a MKMF-1 microphotocolorimeter. Proline and tryptophan are determined separately with the aid of specific test-tube reactions. The sensitivity of the method permits the analysis of 10–15 µg of aminoacid mixture.
KeywordsProline Ninhydrin Protein Hydrolyzate Isatin Amino Nitrogen
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- 1.N. I. Berezovskaya and E. D. Goryachenkova, Vopr. Med. Khimii, No. 2, 155 (1957); A. I. Kharat'yan and G. A. Feigin, Klinich. Meditsina, No. 5, 102 (1964).Google Scholar
- 2.G. N. Zaitseva and N. P. Tyuleneva, Lab. Delo, No. 3, 24 (1958); M. K. Medvedev, P. V. Kugenev, and V. P. Shchuka, Lab. Delo, No. 9, 3 (1961); V. R. Makarova and M. A. Torban, Tr. Moskovskogo Instituta Vaktsin i Syvorotok,18, 188 (1963).Google Scholar
- 3.T. S. Paskhina, The Quantitative Determination of Amino Acids by Means of Paper Chromatography through the Formation of Copper Derivatives of Amino Acids with Ninhydrin [in Russian], Moscow (1959).Google Scholar
- 4.G. S. Katrukha, Zh. P. Trifonova, and A. B. Silaev, Khim. Prirodn. Soedin., 348 (1966).Google Scholar
- 5.I. Todorov, Clinical Investigations in Pediatry [in Bulgarian], Sofia (1968), p. 739.Google Scholar