Conclusions
1. A spectrophotometric method for the quantitative determination of the acetyl groups in acetates of mononucleotides by means of the acid hydroxamic complex with Fe2(SO4)3 has been developed. It has been shown that the determination of the concentration of nucleotide material in the solution being analyzed can be carried out directly in the reaction mixture in the first stage of the reaction of the acetate with hydroxylamine (before the formation of the Fe+++ complex).
2. In the case of uridine, cytidine, and adenosine derivatives, the reaction with hydroxylamine takes place quantitatively in 10 min at 20°C, while for the determination of the acetyl groups in the triacetate of guanosine 2'(3')-phosphate the reaction was carried out at 40°C for 3 h.
Similar content being viewed by others
Literature cited
N. S. Tikhomirova-Sidorova, G. E. Ustyuzhanin, A. P. Kavunenko, E. M. Kogan, and E. A. Pyaivinen, “The synthesis, structure, and properties of polymers,” Transactions of the XV Scientific Conference of the Institute of High-Molecular Weight Compounds, AS USSR [in Russian] (1970), p. 201.
S. N. Tabib, S. Y. Kulkarni, and V. S. Pansare, Microchem. J.,13, 986 (1968).
Biochem. Prep.,8, 14.
D. G. Knorre, N. M. Pustoshilova, N. M. Teplova, and G. G. Shamovskii, Biokhim.,30, 1218 (1965).
N. K. Kochetkov, É. I. Budovskii, and V. D. Domkin, Mol. biol.,1, 558 (1967).
H. Schuster, Mol. biol.,3, 447 (1961).
N. K. Kochetkov, É. I. Budovskii, and N. A. Simukova, Biokhim.,27, 519 (1962).
T. V. Venkstern and A. A. Baev, Absorption Spectra of the Minor Components and Some Oligonucleotides of Ribonucleic Acids [in Russian], Moscow (1967).
Missing in Russian original—Publisher.
Y. Lapidot and H. G. Khorana, J. Am. Chem. Soc.,85, 3852 (1963).
R. Lhormann and H. G. Khorana, J. Am. Chem. Soc.,86, 4188 (1964).
Additional information
Institute of High-Molecular Compounds, Academy of Sciences of the USSR. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 607–612, September–October, 1970.
Rights and permissions
About this article
Cite this article
Pyaivinen, É.A., Tikhomirova-Sidorova, N.S. Quantitative determination of the acetyl groups in nucleotide acetates. Chem Nat Compd 6, 620–624 (1970). https://doi.org/10.1007/BF00563455
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF00563455