Chemistry of Natural Compounds

, Volume 6, Issue 5, pp 620–624 | Cite as

Quantitative determination of the acetyl groups in nucleotide acetates

  • É. A. Pyaivinen
  • N. S. Tikhomirova-Sidorova


1. A spectrophotometric method for the quantitative determination of the acetyl groups in acetates of mononucleotides by means of the acid hydroxamic complex with Fe2(SO4)3 has been developed. It has been shown that the determination of the concentration of nucleotide material in the solution being analyzed can be carried out directly in the reaction mixture in the first stage of the reaction of the acetate with hydroxylamine (before the formation of the Fe+++ complex).

2. In the case of uridine, cytidine, and adenosine derivatives, the reaction with hydroxylamine takes place quantitatively in 10 min at 20°C, while for the determination of the acetyl groups in the triacetate of guanosine 2'(3')-phosphate the reaction was carried out at 40°C for 3 h.


Guanosine Uridine Hydroxylamine Cytidine Alkaline Hydrolysis 


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Copyright information

© Consultants Bureau, a division of Plenum Publishing Corporation 1973

Authors and Affiliations

  • É. A. Pyaivinen
  • N. S. Tikhomirova-Sidorova

There are no affiliations available

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