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Fluorimetric analysis

1. Untersuchung von Fluorescenzindicatoren zur Messung von pH-Werten im Neutralbereich

Fluorimetrische Analyse

1. A study on fluorescent indicators for measuring near neutral (“Physiological”) pH-values

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Zusammenfassung

Nach einer kurzen Besprechung der gewünschten Eigenschaften von Fluorescenzindicatoren werden die Anregungs- und Emissionsmaxima sowie die pKs-Werte von 28 potentiellen Indicatoren genau untersucht. Zusätzlich werden in qualitativer Weise andere Daten (Fluorescenzquantenausbeute, Löslichkeit und Stabilität) angegeben.

Die Indicatoren werden in die Gruppen A und B unterteilt. Solche der Gruppe A besitzen ihr langwelligstes Absorptionsmaximum und die stärkste Fluorescenz in acider Lösung. Jene der Gruppe B absorbieren am langwelligsten und fluorescieren am intensivsten in basischer Lösung. Von den untersuchten Indicatoren werden einige Cumarine und das HPTS (1-Hydroxypyren-3,6,8-trisulfonsäure-trinatriumsalz) als sehr geeignet befunden. Vom HPTS werden die Absorptions- und Fluorescenzspektren, die Abklingzeiten, die pKs-Werte sowie die Fluorescenzeigenheiten als Funktion des pH-Wertes angegeben. Wegen seiner Stabilität, Wasserlöslichkeit, langwelligen Emission und großen Stokes-Verschiebung erscheint HPTS als der pH-Indicator der Wahl.

Summary

The desired properties of fluorescence pH-indicators are shortly summarised. Fluorescence maxima and pKa values of 28 potential indicators are presented in detail, whilst other data (fluorescence quantum yields, solubility and stability) are given qualitatively.

Indicators are divided into groups A and B. Group A compounds exhibit their most long-wave absorption and most intense fluorescence in acidic solution. Group B indicators exhibit their most long-wave absorption and most intense fluorescence in basic solution.

Amongst the indicators investigated, some coumarins and HPTS(1-hydroxy-pyrene-3,6,8-trisulfonate) are considered to be the most suitable ones for both acid-base titrations and precise pH-measurements. The HPTS absorption, excitation and fluorescence spectra, decay times, pKa-values, and fluorescence properties as a function of pH and excitation wavelength are given. As a result of its stability, water solubility, long-wave excitation maximum and large Stokes' shift HPTS appears to be the pH-indicator of choice.

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Authors and Affiliations

  1. Institut für Organische Chemie der Karl Franzens-Universität, A-8010, Graz, Austria

    Otto S. Wolfbeis & Eva Fürlinger

  2. Abteilung Medizin. Meßtechnik, Firma AVL-Prof. List, Kleiststr. 48, A-8020, Graz, Austria

    Herbert Kroneis & Hermann Marsoner

Authors
  1. Otto S. Wolfbeis
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  2. Eva Fürlinger
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  3. Herbert Kroneis
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  4. Hermann Marsoner
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Wolfbeis, O.S., Fürlinger, E., Kroneis, H. et al. Fluorimetric analysis. Z. Anal. Chem. 314, 119–124 (1983). https://doi.org/10.1007/BF00482235

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  • DOI: https://doi.org/10.1007/BF00482235

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