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Filamentous cyanophytes containing phycourobilin and in symbiosis with sponges and an ascidian of coral reefs

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Abstract

A study was made of the ultrastructure and pigment composition of filamentous cyanophytes living in symbiosis with several sponges and a colonial didemnid ascidian collected from the southern end of the Great Barrier Reef, Australia, between 1983 and 1986. The sponges were Dysidea herbacea Keller and several other encrusting sponges which have not been identified; the ascidian was Trididemnum miniatum Kott (1977). The cyanophyte Oscillatoria spongeliae (Shultz) Hauck was identified as the symbiont of several of the sponges, including D. herbacea. Two other unidentified Oscillatoria species were found in a bristly papillate sponge and in T. miniatum. Chlorophyll a, alone, was present in all the symbionts with the exception of T. miniatum, which contained the cosymbiont Prochloron and where chlorophyll b was also present. Two phycoerythrins were isolated by chromatography and chromatofocusing. Both resembled C-phycoerythrin, but one of the two carried the chromophore phycourobilin as well as phycoerythrobilin possibly on both the α and β subunits, which had apparent molecular masses of 18 and 20 kdaltons. No γ subunit was present. Ultrastructurally, the three Oscillatoria species were distinguished by an unusual type of parallel, longitudinal, thylakoid organisation; the arrangement was different in detail in each species.

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Literature cited

  • Alberte, R. A., A. M. Wood, T. A. Kursar and R. L. L. Guillard: Novel phycoerythrins in marine Synechococcus spp. Characterization and evolutionary and ecological implications. Pl. Physiol. 75, 732–739 (1984)

    Google Scholar 

  • Berthold, R. J., M. A. Borowitzka and M. A. Mackay: The ultrastructure of Oscillatoria spongeliae, the blue-green algal endosymbiont of the sponge Dysidea herbacea. Phycologia 21, 327–335 (1982)

    Google Scholar 

  • Bryant, D. A., G. Cohen-Bazire and A. N. Glazer: Characterization of the biliproteins of Gloeobacter violaceus. Chromophore content of a cyanobacterial phycoerythrin carrying phycourobilin chromophore. Archs Microbiol. 129, 190–198 (1981)

    Google Scholar 

  • Cox, G. C., D. C. Benson and D. M. Dwarte: Ultrastructure of a cave wall cyanophyte-Gloeocapsa NS4. Archs Microbiol. 130, 165–174 (1981)

    Google Scholar 

  • Cox, G. C., R. G. Hiller and A. W. D. Larkum: An unusual cyanophyte, containing phycourobilin and symbiotic with sponges and ascidians. Mar. Biol. 89, 149–163 (1985)

    Google Scholar 

  • Fujita, A. and S. Shimura: Phycoerythrin of the marine blue-green alga Trichodesmium thiebautii. Pl. Cell Physiol., Tokyo 15 939–942 (1974)

    Google Scholar 

  • Glazer, A. N.: Phycobilisome. A macromolecular complex optimised for light energy transfer. Biochim. biophys. Acta 768, 29–51 (1984)

    Google Scholar 

  • Glover, H. E., M. D. Keller and R. L. L. Guillard: Light quality and oceanic ultraphytoplankters. Nature, Lond. 319, 142–143 (1986)

    Google Scholar 

  • Golecki, G. R. and G. Drews: Supramolecular organization and composition of membranes. In: The biology of the Cyanobacteria, pp 125–141. Ed. by N. G. Carr and B. A. Whitton. Oxford: Blackwell 1982

    Google Scholar 

  • Hirose, H., S. Kumano and K. Madano: Spectroscopic studies on phycoerythrins from cyanophycean and rhodophycean algae with special reference to their phylogenetic relations. Bot. Mag., Tokyo 82, 197–203 (1969)

    Google Scholar 

  • Johnson, P. W. and J. McN. Sieburth: Chroococcoid cyanobacteria in the sea: a ubiquitous and diverse phototropic biomass. Limnol. Oceanogr. 24, 928–935 (1979)

    Google Scholar 

  • Kott, P. A.: Algal supporting didemnid ascidians of the Great Barrier Reef. Proc. 3rd int. Symp. coral Reefs 1, 615–621 (1977). (Ed. by D. L. Taylor. Miami: School of Marine and Atmospheric Sciences, University of Miami)

    Google Scholar 

  • Kott, P. A.: Algal-bearing didemnid ascidians in the Indo-west-Pacific. Mem. Qd Mus. 20, 1–47 (1980)

    Google Scholar 

  • Kursar, T. A., H. Swift and R. S. Alberte: Morphology of a novel cyanobacterium and characterisation of light-harvesting complexes derived from it: implications for phycobiliprotein evolution. Proc. natn. Acad. Sci. U.S.A. 78, 6888–6892 (1981)

    Google Scholar 

  • Laemmli, U. K.: Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, Lond. 227, 680–685 (1970)

    Google Scholar 

  • Lafargue, F. et G. Duclaux: Premier exemple, en Atlantique tropical, d'une association symbiotique entre une ascidie didemnidae et une cyanophycée chroococcale: Trididemnum cyanophorum nov. sp. et Synechocystis trididemni nov. sp. Annls Inst. océanogr., Paris (N.S.) 55, 163–184 (1979)

    Google Scholar 

  • Larkum, A. W. D. and J. Barrett: Light harvesting systems in algae. Adv. bot. Res. 10, 1–219 (1983)

    Google Scholar 

  • Lewin, R. A.: Prochloron, type genus of the Prochlorophyta. Phycologia 16, p. 217 (1977)

    Google Scholar 

  • MacColl, R.: Phycobilisomes and biliproteins. Photochem. Photobiol. 35, 899–904 (1982)

    Google Scholar 

  • Ong, L. J., A. N. Glazer and J. B. Waterbury: An unusual phycoerythrin from a marine cyanobacterium. Science, N.Y. 224, 80–83 (1984)

    Google Scholar 

  • Parry, D. L.: Cyanophytes with R-phycoerythrins in association with seven species of ascidians from the Great Barrier Reef. Phycologia 23, 503–505 (1984)

    Google Scholar 

  • Stadnichuk, I. N., N. I. Romanova and I. O. Selyakh: A phycobilin-containing phycoerythrin from the cyanobacterium Oscillatoria sp. Archs Microbiol. 143, 20–25 (1985)

    Google Scholar 

  • Sybesma, J. and R. P. M. Bak: The distribution of Trididemnum solidum (van Name), an algae-containing compound ascidian, along the coasts of Curaçao (N.A.). Proc. 14th Meet. Ass. Isl. mar. Labs Caribb. 14, p 6 (1979) (Santo Domingo)

    Google Scholar 

  • Waterbury, J. B., S. W. Watson, R. R. L. Guillard and L. E. Brand: Widespread occurrence of a unicellular, marine, planktonic cyanobacterium. Nature, Lond. 277, 293–294 (1979)

    Google Scholar 

  • Wilkinson, C. R.: Cyanobacteria symbiotic in marine sponges. In: Endocytobiology. pp 553–563. Ed. by W. Schwemmler and H. E. A. Shenk. Berlin: Walter de Gruyter 1980

    Google Scholar 

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Communicated by G. F. Humphrey, Sydney

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Larkum, A.W.D., Cox, G.C., Hiller, R.G. et al. Filamentous cyanophytes containing phycourobilin and in symbiosis with sponges and an ascidian of coral reefs. Mar. Biol. 95, 1–13 (1987). https://doi.org/10.1007/BF00447479

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