Advertisement

Marine Biology

, Volume 102, Issue 4, pp 445–452 | Cite as

RQ of benthic marine invertebrates

  • A. Hatcher
Article

Abstract

This study investigated an incubation method which employed simultaneous measurement of CO2 production and O2 consumption rates to calculate the RQ (respiratory quotient; CO2 production rate: O2 consumption rate) of individual benthic marine invertebrates. Carbon dioxide production rates were calculated from changes in CO2 concentration determined using seawater pH. O2 consumption rates were calculated from changes in O2 concentration with a correction applied for O2 flux across the air/water interface due to gaseous exchange. Species examined were Triphyllozoon sp. cf. moniliferum (MacGillivray 1860), a bryozoan; Herdmania momus (Savigny), a solitary ascidian; Poneroplax albida (Blainville 1825), a chiton; and Haliotis roei (Gray 1826), an abalone. Six individuals of each were collected on 14 November 1985 from the limestone walls of a cave in a nearshore reef off Marmion, Western Australia. After acclimation for 6 h in experimental conditions, rates of CO2 production and O2 consumption were measured. A minimum period of 4 h was required to obtain consistent RQ values for each species. The standard error (SE) of the (calculated) RQ ratio was 14 to 33% of the mean in incubations of 4 h, and less than 14% in incubations of 4 to 12 h. The RQ is commonly used as an indicator of unknown catabolic substrates by comparing it with biochemically determined limits for known substrates. This study provides a strong argument against using the RQ of individual animals to draw any conclusions about catabolic substrates. Unexplained variation in the components of the RQ of an individual, measured over short time periods, and the potential involvement of stored reserves in catabolism, over longer time periods, obscure the relationship between the RQ of individual animals and the ratio's biochemically determined limits.

Keywords

Production Rate Consumption Rate Short Time Period Individual Animal Longe Time Period 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. Beyers, R. J. (1966). Metabolic similarities between symbiotic coelenterates and aquatic ecosystems. Arch. Hydrobiol., 62: 273–284Google Scholar
  2. Beyers, R. J., Larimer, J. L., Odum, H. T., Parker, R. B., Armstrong, N. E. (1963). Directions for the determination of changes in carbon dioxide concentration from changes in pH. Publs. Inst. mar. sci. Univ. Tex. 9: 454–489Google Scholar
  3. Dejours, P. (1981). Principles of comparative respiratory physiology. 2nd ed. Elsevier, HollandGoogle Scholar
  4. Downing, A. L., Truesdale, G. A. (1955). Some factors affecting the rate of solution of oxygen in water. J. Appl. Chem., Lond. 5: 570–581Google Scholar
  5. Hatcher, A. I. (1987). Carbon, nitrogen and phosphorus turnover in the solitary ascidian Herdmania momus (Savigny). Ph.D. thesis, University of Western AustraliaGoogle Scholar
  6. Ikeda, T. (1977). The effect of laboratory conditions on the extrapolation of experimental measurements to the ecology of marine zooplankton. IV. Changes in respiration and excretion rates of boreal zooplankton species maintained under fed and starved conditions. Mar Biol. 41: 241–252Google Scholar
  7. Kendall, M. G., Stuart, A. (1969). The advanced theory of statistics. Vol. I. Distribution theory. Charles. Griffin & Co., LondonGoogle Scholar
  8. Kenney, J. K., Keeping, E. S. (1954). Mathematics of statistics. Part 1. D. VanNostrand, PrincetonGoogle Scholar
  9. Quetin, L. B., Ross, R. M., Uchio, K. (1980). Metabolic characteristics of midwater zooplankton: ammonia excretion, O:N ratios, and the effect of starvation. Mar. Biol. 59: 201–209Google Scholar
  10. Sasaki, G. C., Capuzzo, J. McD., Biesiot, P. (1986). Nutritional and bioenergetic considerations in the development of the American lobster Homarus americanus. Can. J. Fish. aquat. Sciences 43: 2311–2319Google Scholar
  11. Schmidt-Nielsen, K. (1975). Animal physiology. Cambridge Press, LondonGoogle Scholar
  12. Skirrow, G. (1975). The dissolved gases — carbon dioxide. In: Riley, J. P., Skirrow, G. (eds). Chemical oceanography. Vol. 2, 2nd ed. Academic Press, New York, p. 1–92Google Scholar
  13. Smith, S. V., Atkinson, M. J. (1983). Mass balance of carbon and phosphorus in Shark Bay, Western Australia. Limnol. Oceanogr. 28: 625–639Google Scholar
  14. Smith, S. V., Kinsey, D. W. (1978). Calcification and organic carbon metabolism as indicated by carbon dioxide. In: Stoddart, D., Johnnes, R. E. (eds.) Coral reefs: research methods. UNESCO, Manila, p. 469–484Google Scholar
  15. Snow, N. B., Williams, P. J. LeB. (1971). A simple method to determine the O:N ratio of small marine animals. J. Mar. biol. Ass. U.K. 51: 105–109Google Scholar
  16. Sokal, R. R., Rohlf, F. J. (1969). Biometry. The principles and practice of statistics in biological research. W. H. Freeman & Co., San FranciscoGoogle Scholar
  17. Vink, S., Atkinson M. J. (1985). High dissolved C: P excretion ratios for large benthic marine invertebrates. Mar. Ecol. Prog. Ser. 21: 191–195Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • A. Hatcher
    • 1
  1. 1.Marine Biological Laboratories, Department of ZoologyThe University of Western AustraliaNorth BeachAustralia

Personalised recommendations