Abstract
Adult female Calanus helgolandicus were transferred, immediately after collection in the English Channel in June 1984, to two unialgal diets one of which, the flagellate Cryptomonas maculata, was rich in starch, while the other, the diatom Thalassiosira weissflogii, contained no starch. The activity of the digestive enzymes amylase, trypsin and laminarinase was measured in these two populations under foodsaturating conditions over an acclimation period of eight days. Ingestion rates were measured on a daily basis and the results confirmed, together with a constant level of body protein, that the experimental conditions were above the incipient limiting concentration. In the long-term (4 to 8 d), the activities of all three enzymes were significantly elevated in the C. maculata-fed copepods, whereas ingestion rates were lower than those on T. weissflogii. These results observed under food-saturating conditions indicate a compensatory mechanism between digestive enzymes and the substrate ingested. They are consistent with previous work on Artemia sp. suggesting that the rate of assimilation is a function of the digestive enzyme activity and ingestion rate. Enzyme activity exhibited differing shortterm responses (<48 h) on transfer to the two algal diets, which are interpreted in relation to the in situ activity of the field population.
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Literature cited
Baars, M. A. and S. S. Oosterhuis: Diurnal feeding rhythms in North Sea copepods measured by gut fluorescence, digestive enzyme activity and grazing on labelled food. Neth. J. Sea Res. 18, 97–119 (1984)
Båmstedt, U.: Diel variations in the nutritional physiology of Calanus glacialis from Lat. 78°N in the summer. Mar. Biol. 79, 257–267 (1984)
Bittner, D. L. and M. L. Cleary: The cupric-phenanthroline chelate in the determination of monosaccharides in whole blood. Am. J. clin. Path. 11, p. 423 (1963)
Bond, R. M.: Digestive enzymes of the pelagic copepod Calanus finmarchicus. Biol. Bull. mar. biol. Lab., Woods Hole 67, 461–465 (1934)
Boucher, J. et J. F. Samain: L'activité amylasique indice de la nutrition du zooplancton, mise en évidence d'un rhythme quotidien en zone d'upwelling. Téthys 6, 179–188 (1974)
Corring, T. et A. Aumaitre: Mise en place et évolution de l'équipement enzymatique du pancreas exocrine du jeune rat pendant la periode embryonnaire, l'allaitement et le sevrage. Annls Biol. anim. Biochim. Biophys. 10, 431–441 (1970)
Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers and F. Smith: Colorimetric method for determination of sugars and related substances. Analyt. Chem. 28, 350–356 (1956)
Engelmann, F.: Food stimulated synthesis of intestinal proteolytic enzymes in the cockroach Leucophae maderae. J. Insect Physiol. 15, 217–235 (1969)
Frost, B. W.: Effects of size and concentration of food particles on the feeding behaviour of the marine planktonic copepod Calanus pacificus. Limnol. Oceanogr. 17, 805–815 (1972)
Guillard, R. R. L. and J. H. Ryther: Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Gran. Can. J. Microbiol. 8, 229–239 (1962)
Hallberg E. and H. J. Hirche: Differentiation of mid-gut in adults and overwintering copepodids of Calanus finmarchicus (Gunnerus) and Calanus helgolandicus Claus. J. exp. mar. Biol. Ecol. 48, 283–295 (1980)
Hassett, R. P. and M. R. Landry: Digestive carbohydrase activities in individual marine copepods. Mar. Biol. Lett. 3, 211–221 (1982)
Hassett, R. P. and M. R. Landry: Effects of food-level acclimation on digestive enzyme activities and feeding behavior of Calanus pacificus. Mar. Biol. 75, 47–55 (1983)
Head, E. J. H. and R. J. Conover: Induction of digestive enzymes in Calanus hyperboreus. Mar. Biol. Lett. 4, 219–231 (1983)
Hirche, H.-J.: Digestive enzymes of copepodids and adults of Calanus finmarchicus and Calanus helgolandicus in relation to particulate matter. Kieler Meeresforsch. (Sondhft) 5, 174–185 (1981)
Ikeda, T.: Nutritional ecology of marine zooplankton. Mem. Fac. Fish. Hokkaido Univ. 22, 1–97 (1974)
Joint, I. R. and R. K. Pipe: An electron microscope study of a natural population of picoplankton from the Celtic Sea. Mar. Ecol. Prog. Ser. 20, 113–118 (1984)
Lowry, O. H., N. J. Rosenbrough, A. L. Farr and R. J. Randall: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Mayzaud, P. and R. J. Conover: Influence of potential food supply on the activity of digestive enzymes of neritic zooplankton. Proc. 10th Eur. mar. Biol. symp. 2, 415–427 (1975). (Ed. by G Persoone and E. Jaspers. Wetteren, Belgium: Universa Press)
Mayzaud, O., P. Mayzaud, C. de La Bigne and P. Grohan: Diel changes in the particulate environment, feeding activity and digestive enzyme concentration in neritic zooplankton. J. exp. mar. Biol. Ecol. 84, 15–35 (1984)
Mayzaud, P. and S. A. Poulet: The importance of the time factor in the response of zooplankton to varying concentrations of naturally occurring particulate matter. Limnol. Oceanogr. 23, 1144–1154 (1978)
Moal, J., J. E. Samain, C. Koutsikopoulos, J. R. Le Coz and J. Y. Daniel: Ushant thermal front: digestive enzymes and zooplankton production. Proc. 19th Eur. mar. biol. Symp. 145–156. (Ed. by P. E. Gibbs. Cambridge: University Press)
Painter, T. J.: Algal polysaccharides. In: The polysaccharides, Vol. 2. pp 195–285. Ed. by G. O. Aspinall. London: Academic Press 1983
Samain, J. F., J. Y. Daniel et J. R. Le Coz: Trypsine, amylase et proteines du zooplancton: dosage automatique et manuel. J. exp. mar. Biol. Ecol. 29, 279–289 (1977)
Samain, J. F., A. Hernandorena, J. Moal, J. Y. Daniel and J. R. Le Coz: Amylase and trypsin activities during Artemia development on axenic media: effect of starvation and specific deletions. J. exp. mar. Biol. Ecol. 86, 255–270 (1985)
Samain, J. F., A. Hernandorena, A. A. van Wormhoudt, J. Moal, J. Y. Daniel, O. Jestin et J. R. Le Coz: Lactivité amylasique et son substat: conséquences écologiques. Océanis 9, 257–263 (1983)
Samain, J. F. et J. Moal: Activités amylasique et trypsique du zooplankton, indices de nutrition? Perspectives et limites. Publs Cent. natn. Exploit. Océans (CNEXO) (Sér. Act. Colloques) 14, 193–206 (1982)
Samain, J. F., J. Moal, J. Y. Daniel and J. R. Le Coz: Possible processes of nutritive adaptations for zooplankton: a demonstration on Artemia. Kieler. Meeresforsch. (Sondhft) 5, 218–228 (1981)
Snook, J. T. and J. H. Meyer: Responses of digestive enzymes to dietary protein. J. Nutr. 82, 409–414 (1964)
Tande, K. S. and D. Slagstad: Ecological investigation on the zooplankton community of Balsfjorden, Northern Norway. Seasonal and short time variations in enzyme activity in copepodite Stage V and VI males and females of Calanus finmarchicus (Gunnerus). Sarsia 67, 63–68 (1982)
Taylor, F. J. R.: Basic biological features of phytoplankton cells. In: The physiological ecology of phytoplankton, pp 3–55. Ed. by I. Morris. Oxford: Blackwell Scientific Publications 1980
Van Weel, P. B.: On the secretion of digestive enzymes by the marine crab, Thalamita crenata. Z. vergl. Physiol. 43, 567–577 (1960)
Van Wormhoudt, A.: Adaptation des activités digestives de leurs cycles et de leur contrôle aux facteurs du milieu chez Palaemon serratus. Thése d'Etat, Université d'Aix, Marseille II 1980
Yang, Y. and D. M. Davies: Digestion, emphasizing trypsin activity, in adult simuliids (Diptera) fed blood, blood-sucrose mixtures, and sucrose. J. Insect Physiol. 14, 205–222 (1968)
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Communicated by J. Mauchline, Oban
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Harris, R.P., Samain, J.F., Moal, J. et al. Effects of algal diet on digestive enzyme activity in Calanus helgolandicus . Mar. Biol. 90, 353–361 (1986). https://doi.org/10.1007/BF00428559
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DOI: https://doi.org/10.1007/BF00428559