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Marine Biology

, Volume 15, Issue 4, pp 350–355 | Cite as

A method for rearing Pecten maximus larvae in the laboratory

  • Ll. D. Gruffydd
  • A. R. Beaumont
Article

Abstract

Development of the scallop Pecten maximus (L.) from egg to metamorphosis takes 33 to 38 days at 16°C. The shelled veliger first appears 2 days after fertilisation, and crawling pediveligers at around 2 days before metamorphosis. The pediveliger can attach temporarily to filamentous objects by means of a byssus thread and, even after metamorphosis, periods of attachment alternate with periods of crawling. The larvae are reared in polythene bins containing 30 l of full salinity sea water (pre-filtered through 0.2μ filters), which is changed every 2 days. Mixtures of algal foods (Isochrysis galbana, Chaetoceros calcitrans, Pyramimonas obovata and Tetraselmis suecica), concentrated by centrifugation, are added at each change from the third day onwards to give a concentration of 50 cells/μl. Larval density is maintained below 10/ml. Antibiotics are added at each change, but very careful sterilisation of all equipment is necessary to guard against the introduction of diseases.

Keywords

Centrifugation Polythene Larval Density Isochrysis Algal Food 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Ll. D. Gruffydd
    • 1
  • A. R. Beaumont
    • 1
  1. 1.NERC Unit of marine Invertebrate BiologyMarine Science LaboratoriesMenai BridgeUK

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