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Retention of particulate adenosine triphosphate from Mediterranean deep-sea water by glass-fibre and membrane filters

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Abstract

When deep-water samples from the Mediterranean Sea were passed through glass-fibre filters (Whatman GF/F), the adenosine nucleotide concentration of the particulate material was exceptionally low. The concentration was higher when the particulate material was concentrated on SM cellulose-membrane filters with porosities of less than 1 μm. There would seem to be two reasons for this: (1) inhibition of the bioluminescent reaction; (2) an apparently lower retention of ATP by the glass-fibre filters. In order to compare results obtained with the two different types of filters, a correction factor of two can be used if measurements are made with an internal standard. This constant factor can only be applied to samples from depths greater than 300 m.

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Authors and Affiliations

  1. Centre d'Etudes et de Recherches Océanologiques de Villefranche-sur-mer, Université Pierre et Marie Curie, Station Zoologique, BP 28, F-06230, Villefranche-sur-mer, France

    J. Gostan

  2. Laboratoire J. Maetz, Commissariat à l'Energie Atomique, Département de Biologie, Centre d'Etudes et de Recherches Océanologiques de Villefranche-sur-mer, F-06230, Villefranche-sur-mer, France

    N. Mayer-Gostan

  3. Ecologie et Biochimie Microbienne du Milieu Marin, Université de Provence, 3 place Victor Hugo, F-13331, Marseille Cédex 3, France

    J. -C. Romano

Authors
  1. J. Gostan
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  2. N. Mayer-Gostan
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  3. J. -C. Romano
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Communicated by J. M. Pérès, Marseille

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Gostan, J., Mayer-Gostan, N. & Romano, J.C. Retention of particulate adenosine triphosphate from Mediterranean deep-sea water by glass-fibre and membrane filters. Mar. Biol. 86, 213–217 (1985). https://doi.org/10.1007/BF00399029

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  • DOI: https://doi.org/10.1007/BF00399029

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