Abstract
The activities [units-1 wet weight tissue] of lactate dehydrogenase (LDH), pyruvate kinase (PK), malate dehydrogenase (MDH) and isocitrate dehydrogenase (IDH) in white skeletal muscle, brain and heart of 24 pelagic teleost fishes were determined. In addition, for several of the foregoing species, citrate synthase (CS) activities were examined in white skeletal muscle. In muscle, the activities of all these enzymes decrease exponentially with increasing minimum depth of occurrence of the species; this decrease closely parallels the decrease in respiratory rate found previously for these same species. The decline in enzymic activity with increasing minimum depth of occurrence is much greater than the decline in body protein content of the whole fishes, suggesting a disproportionately rapid fall in enzyme concentration in comparison to contractile and structural protein concentrations. The similar reductions in activities of both glycolytic (LDH and PK) and citric acid cycle (CS, MDH and IDH) enzymes with depth indicate that both standard and active metabolisms of deeper-dwelling species are reduced relative to shallower-dwelling forms. There is no suggestion of increased anaerobic capacity with depth or in relation to species, occurrence in the oxygen minimum layer. In brain and heart, there is no significant decrease in enzymic activity with increasing minimum depth of occurrence. These two tissues have similar capacities for metabolism in most fishes, when comparisons are based on enzymic activity per gram wet weight of tissue.
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Literature Cited
Alp, P.R., E.A. Newsholme and V.A. Zammit: Activities of citrate synthase and NAD+-linked and NADP+-linked isocitrate dehydrogenase in muscle from vertebrates and invertebrates. Biochem. J. 154, 689–700 (1976)
Baldwin, J., K.B. Storey and P.W. Hochachka: Lactate dehydrogenase M4 of an abyssal fish: strategies for function at low temperature and high pressure. Comp. Biochem. Physiol. 52B, 19–23 (1975)
Banse, K.: On the vertical distribution of zooplankton in the sea. In: Progress in oceanography, Vol. II. pp 53–125. Ed. by M. Sears. Oxford: Pergamon Press 1964
Barham, E.G.: Deep-sea fishes: lethargy and vertical migration. In: Proceedings of an International Symposium on Biological Sound Scattering in the Ocean, pp 100–118. Ed. by G.B. Farquhar. Washington, D.C.: Maury Center for Ocean Science, Department of the Navy 1971
Blaxter, J.H.S., C.S. Wardle and B.L. Roberts: Aspects of the circulatory physiology and muscle systems of deep-sea fish. J. mar. biol. Ass. U.K. 51, 991–1006 (1971)
Childress, J.J.: Oxygen minimum layer: vertical distribution and respiration of the mysid Gnathophausia ingens. Science, N.Y. 160, 1242–1243 (1968)
Childress, J.J.: The respiratory physiology of the oxygen minimum layer mysid Gnathophausia ingens, 144 pp. Ph.D. dissertation, stanford University 1969
—: Respiratory rate and depth of occurrence of midwater animals. Limnol. Oceanogr. 16, 104–106 (1971a)
—: Respiratory adaptations to the oxygen minimum layer in the bathypelagic mysid Gnathophausia ingens. Biol. Bull. mar. biol. Lab., Woods Hole 141, 109–121 (1971b)
—: The respiratory rates of midwater crustaceans as a function of depth of occurrence and relation to the oxygen minimum layer off Southern California. Comp. Biochem. Physiol. 50A, 787–799 (1975)
—: Effects of pressure, temperature and oxygen on the oxygen-consumption rate of the midwater copepod Gaussia princeps. Mar. Biol. 39, 19–24 (1977a)
—: Physiological approaches to the biology of midwater organisms. In: Oceanic sound scattering prediction, pp 301–324. Ed. by N.R. Anderson and B.J. Zahuranec. New York: Plenum Press 1977b
—, A.T. Barnes, L.B. Quetin and B. Robison: Thermally protecting cod ends for the recovery of living deep-sea animals. Deep-Sea Res. 25, 419–422 (1978)
— and M.H. Nygaard: The chemical composition of midwater fishes as a function of depth of occurrence of Southern California. Deep-Sea Res. 20, 1093–1109 (1973)
——: Chemical composition and buoyancy of midwater crustaceans as function of depth of occurrence off Southern California. Mar. Biol. 27, 225–238 (1974)
Childress, J.J. and G.N. Somero: Increase in glycolytic enzyme activities with increasing size in fishes. (In preparation)
Crabtree, B. and E.A. Newsholme: The activities of nicotinamide-adenine dinucleotide- and nicotinamide-adenine dinucleotide phosphate-linked isocitrate dehydrogenase in insect and vertebrate muscles. Biochem. J. 116, 1–22 (1970)
——: The activities of phosphorylase, hexokinase, phosphofructokinase, lactate dehydrogenase and the glycerol-3-phosphate dehydrogenases in muscles from vertebrates and invertebrates. Biochem. J. 126, 49–58 (1972)
Denton, E.J. and N.B. Marshall: The buoyancy of bathypelagic fishes without a gas-filled swimbladder. J. mar. biol. Ass. U.K. 37, 753–767 (1958)
Douglas, E.L., W.A. Friedl and G.V. Pickwell: Fishes in oxygen minimum zones: blood oxygenation characteristics. Science, N.Y. 191, 957–959 (1976)
Fersht, A.: Enzyme structure and mechanism, 256 pp. San Francisco: W.H. Freeman 1977
Johnston, F.A.: A comparative study of glycolysis in red and white muscles of the trout (Salmo gairdneri) and the mirror carp (Cyprinus carpio). J. Fish Biol. 11, 575–588 (1977)
—, W. Davison and G. Goldspink: Energy metabolism of carp swimming muscles. J. comp. Physiol. 114, 203–216 (1977)
Lehninger, A.L.: Biochemistry, 1104 pp. New York: Worth Publishers 1975
Moon, T.W. and P.W. Hochachka: Temperature and enzyme activity in poikilotherms: isocitrate dehydrogenases in rainbow-trout liver. Biochem. J. 123, 695–705 (1971)
Simon, L.M. and E.D. Robin: Relative anaerobic glycolytic capacity and pyruvate kinase activity of rabbit tissues. Int. J. Biochem. 3, 329–332 (1972)
Somero, G.N.: Interacting effects of temperature and pressures on enzyme function and evolution in marine organisms. In: Biochemical and biophysical perspectives in marine biology, Vol. 4. pp 1–27. Ed. by D.C. Malins and J.R. Sargent. New York: Academic Press 1979
Somero, G.N., J.F. Siebenaller and P.W. Hochachka: Biochemical and physiological adaptations of deepsea animals. In: The seas, Vol. 8. Ed. by G. Rowe. New York: Wiley-Interscience (In press)
Smith, K.L.: Metabolism of abyssopelagic fishes: in situ measurements of the rattail, Coryphaenoides armatus. Nature, Lond. 274, 362–364 (1978)
— and R. Hessler: Respiration of benthopelagic fishes: in situ measurements at 1230 m. Science, N.Y. 184, 72–73 (1974)
Torres, J.J., B.W. Belman and J.J. Childress: Oxygen consumption rates of midwater fishes off California. Deep-Sea Res. 26A, 185–197 (1979)
Zammit, V.A., I. Beis and E.A. Newsholme: Maximum activities and effects of fructose biphosphate on pyruvate kinase from muscles of vertebrates and invertebrates in relation to the control of glycolysis. Biochem. J. 174, 989–998 (1978)
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Communicated by N.D. Holland, La Jolla
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Childress, J.J., Somero, G.N. Depth-related enzymic activities in muscle, brain and heart of deep-living pelagic marine teleosts. Mar. Biol. 52, 273–283 (1979). https://doi.org/10.1007/BF00398141
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DOI: https://doi.org/10.1007/BF00398141