Abstract
Prochloron spp. Lewin, 1977, from three species of ascidians [Lissoclinum patella (Gottschaldt, 1898), Trididemnum cyclops Michaelsen, 1921 and Diplosoma virens (Hartmeyer, 1909)] showed light-dependent assimilation of 15N-labelled ammonium sulphate. The 15N assimilated into glutamine was measured using the non-invasive technique of nitrogen-15 nuclear magnetic resonance (15N NMR).
Similar content being viewed by others
Literature cited
Burns, R. C. and R. W. F. Hardy: Nitrogen fixation in bacteria and higher plants. In: Molecular biology, biochemistry and biophysics, Vol. 21 Ed. by A. Kleinzeller, G. F. Springer and H. G. Wittmann. Berlin: Springer-Verlag 1975
Burris, R. H.: Uptake and assimilation of 15NH +4 by a variety of corals. Mar. Biol. 75, 151–155 (1983)
Carpenter, E. J. and J. L. Culliney: Nitrogen fixation in marine shipworms. Science, N.Y. 187, 551–552 (1975)
Goodbody, I.: Nitrogen excretion in Ascidiacea 1, Excretion of ammonia and total non-protein nitrogen. J. exp. Biol. 34, 297–305 (1957)
Gottschaldt, R.: Synascidien von Ternata. Abh. senckenb. naturforsch. Ges. 24, 641–666 (1898)
Griffiths, D. J. and L.-V. Thinh: Transfer of photosynthetically fixed carbon between the prokaryotic green alga Prochloron and its ascidian host. Aust. J. mar. Freshwat. Res. 34, 431–440 (1983)
Guerinot, M. L., W. Fong and D. G. Patriquin: Nitrogen fixation (acetylene reduction) associated with sea urchins (Strongylocentrotus droebachiensis) feeding on seaweeds and eelgrass J. Fish. Res. Bd Can. 34, 416–420 (1977)
Hartmeyer, R.: Ascidien. Bronn's Kl. Ordn. Tierreichs 3, 89–98 (1909)
Hawkins, C. J., G. A. James, D. L. Parry, J. H. Swinehart and A. L. Wood: Intracellular acidity in the ascidian. Comp. Biochem. Physiol. 76B, 559–565 (1983a)
Hawkins, C. J., P. Kott, D. L. Parry and J. H. Swinehart: Vanadium content and oxidation state related to ascidian phylogeny. Comp. Biochem. Physiol. 76B, 555–558 (1983b)
Kawaguti, S.: Ammonium metabolism of the reef corals. Biol. J. Okayama Univ. 1, 171–176 (1953)
Kott, P.: Algal-bearing didemnid ascidians in the Indo-West Pacific. Mem. Qd Mus. 20, 1–47 (1980)
Kott, P., D. L. Parry and G. C. Cox: Prokaryotic symbionts with a range of ascidian hosts. Bull. mar. Sci. 34, 308–312 (1984)
Lewin, R. A.: Prochloron, type genus of the Prochlorophyta. Phycologia 16, p. 217 (1977)
Macara, I. G., G. C. McLeod and K. Kustin: Vanadium in tunicates: oxygen-binding studies. Comp. Biochem. Physiol. 62A, 821–826 (1979)
Michaelsen, W.: Ascidien vom westlichen Indischen Ozean aus dem Reichs-Museum zu Stockholm. Ark. Zool. 13, 1–25 (1921)
Muscatine, L. and C. F. D'Elia: The uptake, retention and release of ammonium by reef corals. Limnol. Oceanogr. 23, 725–734 (1978)
Paerl, H. W.: N2 fixation (nitrogenase activity) attributable to a specific Prochloron (Prochlorophyta)-ascidian association in Palau, Micronesia. Mar. Biol. 81, 251–254 (1984)
Parry, D. L.: Chemical properties of the test of ascidians in relation to predation. Mar. Ecol. Prog. Ser. 17, 279–282 (1984)
Sabbadin, A. and A. Tondonati: Nitrogenous excretion in the compound ascidians Botryllus schlosseri (Pallas) and Botryllus leach (Savigny). Monitore zool. ital. (N.S.) 1, 185–190 (1967)
Summons, R. E. and C. B. Osmond: Nitrogen assimilation in the symbiotic marine alga Gymnodinium microadriaticum: direct analysis of 15N incorporation by CG-MS methods. Phytochem. 20, 575–578 (1981)
Taylor, D. C.: Nutrition of algal-invertebrate symbiosis. II. Effects of exogenous nitrogen sources on growth, photosynthesis and the rate of excretion by algal symbionts in vivo and in vitro. Proc. R. Soc. (Ser. B) 201, 401–412 (1978)
Thinh, L.-V.: Prochloron (Prochlorophyta) associated with the ascidian Trididemnum cyclops Michaelsen. Phycologia 18, 77–82 (1979)
Thinh, L.-V. and D. J. Griffiths: Studies of the relationship between the ascidian D. virens and its associated microscopic algae. I. Photosynthetic characteristics of the algae. Aust. J. mar. Freshwat. Res. 28, 673–681 (1977)
Thomas, W. H.: Surface nitrogenous nutrients and phytoplankton in the northeastern tropical Pacific Ocean. Limnol. Oceanogr. 11, 393–400 (1966)
Wilkinson, C. R. and P. Fay: Nitrogen fixation in coral reef sponges with symbiotic cyanobacteria. Nature, Lond. 279, 527–529 (1979)
Author information
Authors and Affiliations
Additional information
Communicated by G. F. Humphrey, Sydney
Rights and permissions
About this article
Cite this article
Parry, D.L. Nitrogen assimilation in the symbiotic marine algae Prochloron spp.. Mar. Biol. 87, 219–222 (1985). https://doi.org/10.1007/BF00397797
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00397797