Abstract
The patterns of 14C incorporation into the major endproducts of photosynthesis were measured in the surface waters of a New Zealand coastal upwelling system between 8 March and 5 April, 1983. Overall, the majority of 14C was incorporated into protein (x=43.4%) followed by that into polysaccharide (x=33.3%), lipid (x=18.9%) and low molecular weight (LMW) metabolites (x=4.5%). High relative accumulation of 14C into protein occurred at the expense of 14C flow into storage products (primarily polysaccharide) and, to a lesser degree, LMW metabolites. The lowest proportion of 14C incorporated into protein was generally observed in the productive waters associated with the upwelling center. The proportion of 14C incorporation into protein was positively correlated with seston particulate-N: particulate-P (PN:PP), particulate-N:chlorophyll (PN:chl) and particulate-P:chlorophyll (PP:chl) ratios, but was negatively correlated with assimilation number. Time-course experiments under constant environmental conditions revealed that the photosynthate fractions attained constant proportions in <4h. Under a natural day/night cycle the total incorporation of 14C was paralleled most closely by the 14C flow into polysaccharide, showing increases during the day and decreases at night. This pattern also occurred in the LMW and lipid fractions, although not as marked. In contrast, incorporation into protein continued during both day and night. The results of a pulse-chase experiment implied that polysaccharide was the first major product of photosynthesis and had a turnover time of about 2 h.
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Bowman, M. J., B. A. Foster and P. P. Lappennas: Ocean water properties. In: Maui Development Environmental Study Report on Phase Two, 1977–1981, pp 77–97. Ed. by A. C. Kibblewhite, P. R. Bergquist, B. A. Foster, M. R. Gregory and M. C. Miller. Auckland, New Zealand: University of Auckland 1982. (Published for Shell, BP and Todd Oil Services Ltd Auckland, New Zealand)
Caperon, J. and J. Meyer: Nitrogen limited growth of marine phytoplankton. I. Changes in population characteristics with steady-state growth rate. Deep-Sea Res. 19, 601–618 (1972)
Copin-Montegut, C. and G. Copin-Montegut: Stoichiometry of carbon, nitrogen, and phosphorus in marine particulate matter. Deep-Sea Res. 30, 31–46 (1983)
Downes, M. T.: An automated determination of low reactive phosphorus concentrations in natural waters in the presence of arsenic, silicon, and mercuric chloride. Wat. Res. 12, 743–745 (1978)
Glover, H. E. and I. Morris: Photosynthetic carboxylating enzymes in marine phytoplankton. Limnol. Oceanogr. 24, 510–519 (1979)
Goldman, J. C.: Physiological processes, nutrient availability, and the concept of relative growth rate in marine phytoplankton ecology. In: Primary productivity in the sea, pp 179–194. Ed. by P. G. Falkowski. New York: Plenum Press 1980
Goldman, J. C., J. J. McCarthy and D. G. Peavey: Growth rate influence on the chemical composition of phytoplankton in oceanic waters. Nature Lond. 279, 210–215 (1979)
Hitchcock, G. L.: Photosynthate partitioning in cultured marine phytoplankton. I. Dinoflagellates. J. exp. mar. Biol. Ecol. 69, 21–26 (1983)
Konopka, A. and M. Schnur: Biochemical composition and photosynthetic carbon metabolism of nutrient limited cultures of Merismopedia tenuissima (Cyanophyceae). J. Phycol. 17, 118–122 (1981)
Lehninger, A. L.: Biochemistry, 1104 pp. New York: Worth Publishers 1975
Li, W. K. W., H. E. Glover and I. Morris: Physiology of carbon photoassimilation by Oscillatoria thiebautii in the Caribbean Sea. Limnol. Oceanogr. 25, 447–456 (1980)
Li, W. K. W. and T. Platt: Distribution of carbon among photosynthetic end products in phytoplankton of the eastern Canadian arctic. J. Phycol. 18, 466–471 (1982)
Nydahl, F.: On the optimal conditions for the reduction of nitrate to nitrite by cadmium. Talanta 23, 349–357 (1976)
Miyachi, S., S. Miyachi and A. Kamiya: Wavelength effects on photosynthetic carbon metabolism in Chlorella. Pl. Cell Physiol., Tokyo 19, 277–288 (1978)
Morris, I.: Paths of carbon assimilation in marine phytoplankton. In: Primary productivity in the sea, pp 139–159. Ed. by P. G. Falkowski. New York: Plenum Press 1980
Morris, I.: Photosynthetic products, physiological state, and phytoplankton growth. Can. Bull. Fish. aquat. Sciences 210, 83–102 (1981)
Morris, I., H. E. Glover and C. S. Yentsch: Products of photosynthesis by marine phytoplankton: the effect of environmental factors on the relative rates of protein synthesis. Mar. Biol. 27, 1–9 (1974)
Morris, I. and W. Skea: Products of photosynthesis in natural populations of marine phytoplankton from the Gulf of Maine. Mar. Biol. 47, 303–312 (1978)
Morris, I., A. E. Smith and H. E. Glover: Products of photosynthesis in phytoplankton off the Orinoco River and in the Caribben Sea. Limnol. Oceanogr. 26, 1034–1044 (1981)
Oaks, A. and R. G. S. Bidwell: Compartmentation of intermediary metabolites. A. Rev. Pl. Physiol. 21, 43–66 (1970)
Parsons, T. R., K. Stephens and J. D. H. Strickland: On the chemical composition of eleven species of marine phytoplankton. J. Fish. Res. Bd Can. 18, 1001–1016 (1961)
Priscu, J. C. and C. R. Goldman: Suspensoid characteristics in subalpine Castle Lake, California. I. Chemical composition. Arch. Hydrobiol. 97, 373–388 (1983a)
Priscu, J. C. and C. R. Goldman: Carboxylating enzyme activity and photosynthetic end-products of phytoplankton in the shallow and deep-chlorophyll layers of Castle Lake. Limnol. Oceanogr. 28, 1168–1181 (1983b)
Redalje, D. G. and E. A. Laws: The effect of environmental factors on growth and the chemical and biochemical composition of marine diatoms. I. Light and temperature effects. J. exp. mar. Biol. Ecol. 68, 59–79 (1983)
Redfield, A. C., B. H. Ketchum and F. A. Richards: The influence of organisms on the composition of seawater. In: The sea, Vol. 2. pp 26–77. Ed. by M. N. Hill. New York: Wiley Interscience 1963
Searle, P. L.: Automated colorimetric determination of ammonium ions in soil extracts with “Technicon Autoanalyzer II” equipment. N. Z. Jl agric. Res. 18, 183–187 (1975)
Smith, A. E. and I. Morris: Pathways of carbon assimilation in phytoplankton from the Antarctic Ocean. Limnol. Oceanogr. 25, 865–872 (1980)
Solórzano, L.: Determination of ammonium in natural waters by the phenolhypochlorite method. Limnol. Oceanogr. 14, 799–801 (1980)
Stainton, M. P.: Simple, efficient reduction column for use in automated determination of nitrate in water. Analyt. Chem. 46, p. 1616 (1974)
Strickland, J. D. H. and T. R. Parsons: A practical handbook of seawater analysis. Bull. Fish. Res. Bd Can. 167, 1–311 (1968)
Terry, K. L., J. Hirata and E. A. Laws: Light-limited growth of two strains of the marine diatom Phaeodactylum tricornutum Bohlin: chemical composition, carbon partitioning and the diel periodicity of physiological processes. J. exp. mar. Biol. Ecol. 68, 209–227 (1983)
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Communicated by G.F. Humphrey, Sydney
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Priscu, J.C., Priscu, L.R. Photosynthate partitioning by phytoplankton in a New Zealand coastal upwelling system. Mar. Biol. 81, 31–40 (1984). https://doi.org/10.1007/BF00397622
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DOI: https://doi.org/10.1007/BF00397622