Abstract
Glycolate excreted by phytoplankton is a potentially important nutrient for bacteria in coastal and estuarine environments. The metabolism of glycolate by Pseudomonas sp., strain S227, originally isolated from the New York Bight Apex, has been studied. The specific growth rate for this strain on glycolate is 0.156 doublings h-1. The apparent Vmax and Km for glycolate uptake are 83.6 nmol min-1 mg cell protein-1 and 7.4x10-8 M, respectively. The preferential respiration of the carboxyl carbon (C-1) and the incroporation of the hydroxymethyl carbon (C-2) suggest that the glycerate pathway is used for growth on glycolate. Alternatively, another pathway can be utilized which results in the complete catabolism of glycolate. Glycolate and lactate metabolism are also closely linked either by a common metabolic pathway or a common transport system other than the monocarboxylate transport system. The magnesium ion concentration is also important in glycolate metabolism. The characteristics of glycolate metabolism observed in Pseudomonas sp., strain S227, are advantageous in coastal and estuarine environments where glycolate production is intermittent, and the concentrations are low.
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Literature cited
Baumann, P., L. Baumann and M. Marshall: Taxonomy of marine bacteria: the genus Beneckea. J. Bacteriol. 107, 268–294 (1971)
Baumann, L., P. Baumann, M. Mandel and R. D. Allen: Taxonomy of aerobic marine eubacteria. J. Bacteriol. 110, 402–429 (1972)
Beudeker, R. F., J. G. Kuenen and G. A. Codd: Glycollate metabolism in the obligate chemolithotroph Thiobacillus neapolitanus grown in continuous culture. J. gen. Microbiol. 126, 337–346 (1980)
Billen, G., C. Joiris, J. Wijnant and G. Gillain: Concentration and microbiological utilization of small organic molecules in the Scheldt estuary, the Belgian coastal zone of the North Sea and the English Channel. Estuar. cstl mar. Sci. 11, 279–294 (1980)
Bradford, M. M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principal of protein-dye binding. Anal. Biochem. 72, 248–254 (1976)
Chang, W. H. and N. E. Tolbert: Excretion of glycolate, mesotartrate and isocitrate lactone by synchronized cultures of Ankistrodesmus braunii. Plant Physiol. 46, 377–385 (1970)
Codd, G. A., B. Dowien and H. G. Schlegel: Glycolate production and excretion by Alcaligenes eutrophus. Arch. Microbiol. 110, 167–171 (1976)
Corpe, W. A. and R. W. Stone: Oxidation of glycolic acid by Penicillium chrysogenum. J. Bacteriol. 80, 452–456 (1960)
Coughlan, S. J. and R. H. Al-Hasan: Studies of uptake and turnover of glycollic acid in the Menai Straits, North Wales. J. Ecol. 65, 731–746 (1977)
Hansen, R. W. and J. A. Hayashi: Glycolate metabolism in Escherichia coli. J. Bacteriol. 83, 679–687 (1962)
Kay, W. W.: Transport of carboxylic acids, pp 385–411. In: Bacterial transport. Ed. by B. P. Rosen, New York: Marcel Dekker, Inc., 1978
Konings, W. N.: Active transport of solutes in bacterial membrane vesicles. Adv. microb. Physiol. 15, 175–251 (1977)
Kornberg, H. L. and S. R. Elsden: The metabolism of 2 carbon compounds by microorganisms. Adv. Enzymol. 23, 401–470 (1961)
Kornberg, H. L. and A. M. Gotto: The metabolism of C-2 compounds in microorganisms. 6. Synthesis of cell constituents from glycollate by Pseudomonas sp. Biochem. J. 78, 69–82 (1961)
Kornberg, H. L. and J. G. Morris: The utilization of glycollate by Micrococcus denitrificans: the beta-hydroxy-aspartate pathway. Biochem. J. 95, 577–586 (1965)
Kornberg, H. L. and J. R. Sadler: Microbial oxidation of glycollate via a dicarboxylic acid cycle. Nature, Lond. 185, 153–155 (1960)
Kurz, W. G. W. and T. A. G. LaRue: Metabolism of glycolic acid by Azotobacter chroococcum PRL 6H2. Can. J. Microbiol. 19, 321–324 (1973)
Lehninger, A. L.: Biochemistry, Second ed., 1140 pp. New York: Worth Publishers, Inc. 1975
Litchfield, C. D., J. B. Rake, J. Zindulis, R. T. Watanabe and D. C. Stein: Optimization of procedures for the recovery of heterotrophic bacteria from marine sediments. Microbial. Ecol. 1, 219–233 (1975)
MacLeod, R. A.: The question of the existence of specific marine bacteria. Bacteriol. Rev. 29, 9–23 (1965)
Ornston, L. N. and M. K. Ornston: Glycolate uptake by mutant strains of Escherichia coli K-12. J. Bacteriol. 101, 1088–1089 (1970)
Peltzer, E. T. and J. L. Bada: Low molecular weight alphahydroxy carboxylic and dicarboxylic acids in reducing marine sediments. Geochim. cosmochim. Acta 45, 1847–1854 (1981)
Whittingham, C. P. and G. G. Pritchard: The production of glycollate during photosynthesis in Chlorella. Proc. R. Soc. Lond. Ser. B. 157, 366–382 (1963)
Wright, R. T. and N. M. Shah: The trophic role of glycolic acid in coastal seawater. I. Heterotrophic metabolism in seawater and bacterial cultures. Mar. Biol. 33, 175–183 (1975)
Wright, R. T. and N. M. Shah: The trophic role of glycolic acid in coastal seawater. II. Seasonal changes in concentration and heterotrophic use in Ipswich Bay, Massachusetts, USA. Mar. Biol. 43, 257–263 (1977)
Zar, J. H.: Biostatistical analysis, 620 pp. New York: Prentice-Hall, Inc. 1974
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Communicated by O. Kinne, Oldendorf/Luhe
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Edenborn, H.M., Litchfield, C.D. Glycolate metabolism by Pseudomonas sp., strain S227, isolated from a coastal marine sediment. Mar. Biol. 88, 199–205 (1985). https://doi.org/10.1007/BF00397167
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DOI: https://doi.org/10.1007/BF00397167