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Marine Biology

, Volume 63, Issue 1, pp 67–71 | Cite as

Further 237Pu experiments with the plaice Pleuronectes platessa: Subcellular distribution of plutonium in the liver

  • D. R. P. Leonard
  • R. J. Pentreath
Article

Abstract

The subcellular distribution of 237Pu in the livers of the plaice Pleuronectes platessa has been studied by differential and isopycnic gradient centrifugation. It has been shown that the 237Pu is particularly accumulated in pellets sedimenting out at 3000 and 6000 g which, by deduction from marker enzyme tests, contain a mixture of mitochondria and lysosomes. Separation of lysosomes from mitochondria was achieved by using a non-lytic detergent injected into the fish 3 d prior to removal of the livers; it was found that 237Pu was associated with the lysosomal fraction. In this respect, the metabolism of plutonium of the plaice resembles that of the rat.

Keywords

Enzyme Centrifugation Plutonium Gradient Centrifugation Subcellular Distribution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • D. R. P. Leonard
    • 1
  • R. J. Pentreath
    • 1
  1. 1.Ministry of AgricultureFisheries and Food, Directorate of Fisheries Research, Fisheries Radiobiological LaboratoryLowestoftEngland

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