Marine Biology

, Volume 63, Issue 1, pp 67–71 | Cite as

Further 237Pu experiments with the plaice Pleuronectes platessa: Subcellular distribution of plutonium in the liver

  • D. R. P. Leonard
  • R. J. Pentreath


The subcellular distribution of 237Pu in the livers of the plaice Pleuronectes platessa has been studied by differential and isopycnic gradient centrifugation. It has been shown that the 237Pu is particularly accumulated in pellets sedimenting out at 3000 and 6000 g which, by deduction from marker enzyme tests, contain a mixture of mitochondria and lysosomes. Separation of lysosomes from mitochondria was achieved by using a non-lytic detergent injected into the fish 3 d prior to removal of the livers; it was found that 237Pu was associated with the lysosomal fraction. In this respect, the metabolism of plutonium of the plaice resembles that of the rat.


Enzyme Centrifugation Plutonium Gradient Centrifugation Subcellular Distribution 
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Literature Cited

  1. Bessey, O. A., O. H. Lowry and M. H. Brock: A method for the rapid determination of alkaline phosphatase with 5 cubic millimetres of serum. J. biol. Chem. 164, 321–329 (1946)Google Scholar
  2. Boocock, G., C. J. Danpure, D. S. Popplewell and D. M. Taylor: The subcellular distribution of plutonium in rat liver. Radiat. Res. 42, 381–396 (1970)Google Scholar
  3. Bradford, M. M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Analyt. Biochem. 72, 248–254 (1976)Google Scholar
  4. Bruenger, F. W., B. J. Stover and W. Stevens: The subcellular distribution of 139Pu(IV) in the canine liver. Radiat. Res. 43, p.240 (1970)Google Scholar
  5. Bruenger, F. W., B. J. Stover and W. Stevens: 239Pu(IV): its subcellular distribution and association with ferritin in the canine liver. Hlth Phys. 21, 679–687 (1971)Google Scholar
  6. Cooperstein, S. J. and A. Lazarow: A microspectrophotometric method for the determination of cytochrome oxidase. J. Biol. Chem. 189, 665–670 (1951)Google Scholar
  7. Danpure, C. J. and D. M. Taylor: The effect of internally deposited plutonium-239 on the lysosomes of rat liver. Radiat. Res. 59, 679–692 (1974)Google Scholar
  8. Dingle, J. T. (Ed): Lysosomes, a laboratory handbook, 2nd ed. North Holland: Elsevier 1977Google Scholar
  9. Fiske, C. H. and Y. Subbarow: The colorimetric determination of phosphorus. J. biol. Chem. 66, 375–400 (1925)Google Scholar
  10. Guary, J.-C., M. Masson et A. Fraizier: Etude préliminarie, in situ, de la distribution du plutonium dans différents tissue et organes de Cancer pagurus (Crustacea: Decapoda) et de Pleuronectes platessa (Pisces: Pleuronectidae). Mar. Biol. 36, 13–17 (1976)Google Scholar
  11. Gurney, J. R. and D. M. Taylor: The subcellular distribution of 239Pu in rat liver parenchymal cells after exposure in vivo and in vitro. Hlth Phys. 29, 655–661 (1975)Google Scholar
  12. Leighton, F., B. Poole, H. Beaufay, P. Baudhuin, J. W. Coffey, S. Fowler and C. de Duve: The large-scale separation of peroxisomes, mitochondria and lysosomes from the livers of rats injected with Triton WR-1339. J. Cell Biol. 37, 482–513 (1968)Google Scholar
  13. Pentreath, R. J.: 237Pu experiments with the plaice Pleuronectes platessa. Mar. Biol. 48, 327–335 (1978)Google Scholar
  14. Pentreath, R. J. and M. B. Lovett: Occurrence of plutonium and americium in plaice from the north-eastern Irish Sea. Nature, Lond. 262 (5571), 814–816 (1976)Google Scholar
  15. Pentreath, R. J. and M. B. Lovett: Transuranic nuclides in plaice (Pleuronectes platessa) from the north-eastern Irish Sea. Mar. Biol. 48, 19–26 (1978)Google Scholar
  16. Pépin, G. et C. Boudène: Localisation subcellulaire du citrate de plutonium (239Pu) dans l'hépatocyte du rat. C. r. hebd. Séanc. Acad. Sci., Paris 279, 2071–2074 (1974)Google Scholar
  17. Rahman, Y. E. and A. Lindenbaum: Lysosome particles and subcellular distributions of polymeric tetravalent plutonium-239. Radiat. Res. 21, 575–583 (1964)Google Scholar
  18. Seidel, A., R. Winter, C. Jentzsch, R. Gruner, H. G. Heumann and S. Hanke: Studies on the biochemical mechanisms responsible for differences in the half-life of monomeric 239Pu in rat and Syrian hamster liver, Vienna: International Atomic Energy Agency 26–30 March, 1979. (Int. atom. Energy Ag. Sm 237/21)Google Scholar
  19. Siegel, A. and R. J. Bing: Plasma enzyme activity in myocardial infarction in dog and man. Proc. Soc. exp. Biol. Med. 91, 604–607 (1956)Google Scholar
  20. Taylor, D. M.: The metabolism of plutonium in adult rabbits. Br. J. Radiol. 42, 44–50 (1969)Google Scholar
  21. Warburton, M. J. and C. H. Wynn: The uptake of Triton-WR-1339 and various lipids by liver lysosomes of the Syrian hamster (Mesocricetus auratus). Int. J. Biochem. 8, 305–310 (1977)Google Scholar
  22. Wattiaux, R. M., M. Wito and P. Baudhuin: Influence of the injection of Triton WR-1339 on the properties of rat liver lysosomes. In: Ciba Foundation Symposium on Lysosomes, pp 176–200. Ed. by A. V. S. de Reuck and M. P. Cameron. London: J. A. Churchill 1963Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • D. R. P. Leonard
    • 1
  • R. J. Pentreath
    • 1
  1. 1.Ministry of AgricultureFisheries and Food, Directorate of Fisheries Research, Fisheries Radiobiological LaboratoryLowestoftEngland

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