Abstract
Ambient concentrations of urea in the inner Oslofjord, Norway, showed a pronounced yearly cycle in 1980, with values in the range 0.1 to 10.0 μg-at N l-1; this cycle resemble that of ammonia although urea concentrations were usually lower. The uptake of urea by phytoplankton was investigated using 15N. Urea was usually a less important N source than NH +4 , and accounted for 0 to 53% (mean 19%) of summed NH +4 +NO -3 + urea uptake rates from April to October. Absolute as well as relative (specific) uptake rates of urea were higher in the summer (June–August) than at other times. Uptake of urea was inhibited by NH +4 concentrations higher than 1 to 2 μg-at N l-1. The summed NH +4 +NO -3 + urea uptake rate was exponentially related to temperature.
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Antia, N. J., B. R. Berland, D. J. Bonin and S. Y. Maestrini: Comparative evaluation of certain organic and inorganic sources of nitrogen for phototrophic growth of marine microalgae. J. mar. biol. Ass. U.K. 55, 519–539 (1975)
Caperon, J. and J. Meyer: Nitrogen-limited growth of marine phytoplankton-II. Uptake kinetics and their role in nutrient limited growth of phytoplankton. Deep Sea Res. 19, 619–632 (1972)
Carpenter, E. J., C. C. Remsen and B. W. Schroeder: Comparison of laboratory and in situ measurements of urea decomposition by a marine diatom. J. exp. mar. Biol. Ecol. 8, 259–264 (1972a)
Carpenter, E. J., C. C. Remsen and S. W. Watson: Utilization of urea by some marine phytoplankters. Limnol. Oceanogr. 17, 265–269 (1972b)
Collos, Y. and G. Slawyk: 13C and 15N uptake by marine phytoplankton. I. Influence of nitrogen source and concentration in laboratory cultures of diatoms. J. Phycol. 15, 186–190 (1979)
Conway, H. L.: Interactions of inorganic nitrogen in the uptake and assimilation by marine phytoplankton. Mar. Biol. 39, 221–232 (1977)
Eppley, R. W.: Temperature and phytoplankton growth in the sea. Fish. Bull. U.S. 70, 1063–1085 (1972)
Eppley, R. W., A. F. Carlucci, O. Holm-Hansen, D. Kiefer, J. J. McCarthy, E. Venrick and P. M. Williams: Phytoplankton growth and composition in shipboard cultures supplied with nitrate, ammonium, or urea as the nitrogen source. Limnol. Oceanogr. 16, 741–751 (1971)
Eppley, R. W., E. H. Renger, E. L. Venrick and M. M. Mullin: A study of plankton dynamics and nutrient cycling in the central gyre of the North Pacific Ocean. Limnol. Oceanogr. 18, 534–551 (1973)
Eppley, R. W., J. H. Sharp, E. H. Renger, M. J. Perry and W. G. Harrison: Nitrogen assimilation by phytoplankton and other microorganisms in the surface waters of the central North Pacific Ocean. Mar. Biol. 39, 111–120 (1977)
Glibert, P. M. and J. C. Goldman: Rapid ammonium uptake by marine phytoplankton. Mar. Biol. Lett. 2, 25–31 (1981)
Goldman, J. C.: Physiological processes, nutrient availability, and the concept of relative growth rate in marine phytoplankton ecology. In. Primary productivity in the sea, pp 179–194. Ed. by P. G. Falkowski. New York: Plenum Press 1980
Grant, B. R., J. Madgwick and G. Dal Pont: Growth of Cylindrotheca closterium var. californica (Mereschk.) Reimann & Lewin on nitrate, ammonia, and urea. Aust. J. mar. freshwat. Res. 18, 129–135 (1967)
Harvey, W. A. and J. Caperon: Rate of utilization of urea, ammonium, and nitrate by natural populations of marine phytoplankton in a eutrophic environment. Pacif. Sci. 30, 329–340 (1976)
Horrigan, S. G. and J. J. McCarthy: Urea uptake by phytoplankton at various stages of nutrient depletion. J. Plankton Res. 3, 403–414 (1981)
Horrigan, S. G. and J. J. McCarthy: Phytoplankton uptake of ammonium and urea during growth on oxidized forms of nitrogen. J. Plankton Res 4, 379–389 (1982)
Kirk, D. L. and M. M. Kirk: Amino acid and urea uptake in ten species of Chlorophyta. J. Phycol. 14, 198–203 (1978)
Koroleff, F.: Determination of nutrients. In: Methods of seawater analysis, pp 117–181. Ed. by K. Grasshoff. Weinheim: Verlag Chemine 1976
Kristiansen, S.: Nitrate reductase in the inner Oslofjord and in culture experiments (in Norwegian). Thesis, Univ. of Oslo 1978
Kristiansen, S.: The temperature optimum of the nitrate reductase assay on marine phytoplankton. Limnol. Oceanogr. (In press)
Kristiansen, S. and E. Paasche: Preparation of 15N-labeled phytoplankton samples for optical emission spectrometry. Limnol. Oceanogr. 27, 373–375 (1982)
Liu, M. S. and J. A. Hellebust: Utilization of amino acids as nitrogen sources, and their effects on nitrate reductase in the marine diatom Cyclotella cryptica. Can. J. Microbiol 20, 1119–1125 (1974)
McCarthy, J. J.: A urease method for urea in seawater. Limnol. Oceanogr. 15, 309–313 (1970)
McCarthy, J. J.: The uptake of urea by natural populations of marine phytoplankton. Limnol. Oceanogr. 17, 738–748 (1972a)
McCarthy, J. J.: The uptake of urea by marine phytoplankton. J. Phycol. 8, 216–222 (1972b)
McCarthy, J. J.: Nitrogen. In: The physiological ecology of phytoplankton, pp 191–233. Ed. by. I. Morris. Oxford: Blackwell 1980
McCarthy, J. J. and R. W. Eppley: A comparison of chemical, isotopic, and enzymatic methods for measuring nitrogen assimilation of marine phytoplankton. Limnol. Oceanogr. 17, 371–382 (1972)
McCarthy, J. J. and J. C. Goldman: Nitrogenous nutrition of marine phytoplankton in nutrient-depleted waters. Science, N.Y. 203, 670–672 (1979)
McCarthy, J. J., W. R. Taylor and J. L. Taft: The dynamics of nitrogen and phosphorus cycling in the open waters of the Chesapeake Bay. In: Marine chemistry in the coastal environment, pp 664–681. Ed. by T. M. Church. Washington: American Chemical Society 1975
McCarthy, J. J., W. R. Taylor and J. L. Taft: Nitrogenous nutrition of the plankton in the Chesapeake Bay I. Nutrient availability and phytoplankton preferences. Limnol. Oceanogr. 22, 996–1011 (1977)
Mitamura, O. and Y. Saijo: In situ measurement of the urea decomposition rate and its turnover rate in the Pacific Ocean. Mar. Biol. 58, 147–152 (1980)
Morris, I.: Nitrogen assimilation and protein synthesis. In: Algal physiology and biochemistry, pp 583–609. Ed. by W. D. P. Stewart. Oxford: Blackwell 1974
Newell, B. S., B. Morgan and J. Cundy: The determination of urea in seawater. J. mar. Res. 25, 201–202 (1967)
Paasche, E. and S. Kristiansen: Nitrogen nutrition of the phytoplankton in the Oslofjord. Estuar. coast Shelf Sci. 14, 237–249 (1982)
Parsons, T. R.: Particulate organic carbon in the sea. In: Chemical oceanography, Vol. 2, 2nd. ed., pp 365–383. Ed. by J. P. Riley and G. Skirrow. London: Academic Press 1975
Remsen, C. C.: The distribution of urea in coastal and oceanic waters. Limnol. Oceanogr. 16, 732–740 (1971)
Remsen, C. C., E. J. Carpenter and B. W. Schroeder The role of urea in marine microbial ecology. In: Effect of the ocean environment on microbial activities, pp 286–304. Ed by R. R. Colwell and R. Y. Morita. Baltimore: University Park Press 1974
Sakshaug, E. and O. Holm-Hansen: Chemical composition of Skeletonema costatum (Grev.) Cleve and Pavlova (Monochrysis) lutheri (Droop) Green as a function of nitrate-, phosphate-, and iron-limited growth. J. exp. mar. Biol. Ecol. 29, 1–34 (1977)
Strickland, J. D. H. and T. R. Parsons: A practical handbook of seawater analysis. Bull. Fish. Res. Bd Can. 167, 2nd. ed., 1–310 (1972)
Webb, K. L. and L. W. Haas: The significance of urea for phytoplankton nutrition in the York River, Virginia. In: Estuarine processes. Vol. 1. Uses, stresses and adaptation to the estuary, pp 90–102. Ed. by M. Wiley. New York: Academic Press 1976
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Communicated by T. Fenchel, Aarhus
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Kristiansen, S. Urea as a nitrogen source for the phytoplankton in the Oslofjord. Mar. Biol. 74, 17–24 (1983). https://doi.org/10.1007/BF00394270
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DOI: https://doi.org/10.1007/BF00394270