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Marine Biology

, Volume 50, Issue 3, pp 255–261 | Cite as

Changes in the biochemical composition of the ovaries of the seastar Asterias rubens during its annual reproductive cycle

  • R. C. H. M. Oudejans
  • I. van der Sluis
Article

Abstract

The biochemical composition of the ovaries of the asteroid echinoderm Asterias rubens was studied, with emphasis on the changes which are manifest during the annual reproductive cycle. Ovarian dry weight displays only minor changes during ovarian development, constituting about 20% of the organ fresh weight. Levels of all constituents show some variation, but in general increase with ovarian growth. Only the free amino acid level is rather constant. Except for glycogen, the contents of other constituents (total lipids, other reducing sugars, free amino acids and proteins) show a rectilinear increase with ovarian growth. Glycogen content increases rectilinearly with time during early vitellogenesis, but remains constant during further development until ovarian maturity. By plotting constituent levels and contents against a non-linear gonad index axis rather than against sampling date, results can be correlated more easily with ovarian growth stages. The possibility of endocytosis of a vitellogenin-like yolk precursor by the ovary is discussed.

Keywords

Lipid Growth Stage Total Lipid Acid Level Free Amino Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature Cited

  1. Bligh, E.G. and W.J. Dyer: A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911–917 (1959)Google Scholar
  2. Boolootian, R.A.: Reproductive physiology. In: Physiology of Echinodermata, pp 561–613. Ed. by R.A. Boolootian. New York: Interscience Publishers 1966Google Scholar
  3. Ferguson, J.C.: The role of free amino acids in nitrogen storage during the annual cycle of a starfish. Comp. Biochem. Physiol. 51A, 341–350 (1975a)Google Scholar
  4. —: Fatty acid and carbohydrate storage in the annual reproductive cycle of Echinaster. Comp. Biochem. Physiol. 52A, 585–590 (1975b)Google Scholar
  5. —: The annual cycle of fatty acid composition in a starfish. Comp. Biochem. Physiol. 54B, 249–252 (1976)Google Scholar
  6. Gellissen, G., E. Wajc, E. Cohen, H. Emmerich, S.W. Applebaum and J. Flossdorf: Purification and properties of oocyte vitellin from the migratory locust. J. comp. Physiol. 108, 287–301 (1976)Google Scholar
  7. Giese, A.C.: On the biochemical constitution of some echinoderms. In: Physiology of Echinodermata, pp 757–796. Ed. by R.A. Boolootian. New York: Interscience Publishers 1966Google Scholar
  8. Giordano, M.F., H.A. Harper and F.P. Filice: The amino acids of a starfish and a sea urchin (Asteroidea and Echinoidea). Wasmann J. Biol. 8, 129–132 (1950)Google Scholar
  9. Greenfield, L., A.C. Giese, A. Farmanfarmaian and R.A. Boolootian: Cyclic biochemical changes in several echinoderms. J. exp. Zool. 139, 507–524 (1958)Google Scholar
  10. Lawrence, J.M.: Level, content, and caloric equivalents of the lipid, carbohydrate, and protein in the body components of Luidia clathrata (Echinodermata: Asteroidea: Platyasterida) in Tampa Bay. J. exp. mar. Biol. Ecol. 11, 263–274 (1973)Google Scholar
  11. Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)Google Scholar
  12. Moore, S. and W.H. Stein: Photometric ninhydrin method for use in the chromatography of amino acids. J. biol. Chem. 176, 367–387 (1948)Google Scholar
  13. Raven, C.P.: Oogenesis: the storage of developmental information, 274 pp. Oxford: Pergamon Press 1961Google Scholar
  14. Schoenmakers, H.J.N.: The annual reproductive cycle of the ovaries of Asterias rubens (Echinodermata). (In preparation)Google Scholar
  15. Seifter, S., S. Dayton, B. Novic and E. Muntwyler: The estimation of glycogen with the anthrone reagent. Archs Biochem. 25, 191–200 (1950)Google Scholar
  16. Van Handel, E.: Microseparation of glycogen, sugars, and lipids. Analyt. Biochem. 11, 266–271 (1965)Google Scholar
  17. Voogt, P.A. and R. Huiskamp: Sex-dependence and seasonal variation of saponins in the gonads of the starfish Asterias rubens: their relation to reproduction. Comp. Biochem. Physiol. (In press)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • R. C. H. M. Oudejans
    • 1
  • I. van der Sluis
    • 1
  1. 1.Laboratory of Chemical Animal PhysiologyUniversity of UtrechtUtrechtThe Netherlands

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