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Marine Biology

, Volume 85, Issue 3, pp 269–278 | Cite as

Effect of nitrogen supply on nitrogen uptake, accumulation and assimilation in Porphyra perforata (Rhodophyta)

  • T. E. Thomas
  • P. J. Harrison
Article

Abstract

Porphyra perforata J. Ag. was collected from a rocky land-fill site near Kitsilano Beach, Vancouver, British Columbia, Canada and was grown for 4 d in media with one of the following forms of inorganic nitrogen: NO 3 - , NH 4 + and NO 3 - plus NH 4 + and for 10 d in nitrogen-free media. Internal nitrogen accumulation (nitrate, ammonium, amino acids and soluble protein), nitrate and ammonium uptake rates, and nitrate reductase activity were measured daily. Short initial periods (10 to 20 min) of rapid ammonium uptake were common in nitrogen-deficient plants. In the case of nitrate uptake, initial uptake rates were low, increasing after 10 to 20 min. Ammonium inhibited nitrate uptake for only the first 10 to 20 min and then nitrate uptake rates were independent of ammonium concentration. Nitrogen starvation for 8 d overcame this initial suppression of nitrate uptake by ammonium. Nitrogen starvation also resulted in a decrease in soluble internal nitrate content and a transient increase in nitrate reductase activity. Little or no decrease was observed in internal ammonium, total amino acids and soluble protein. The cultures grown on nitrate only, maintained high ammonium uptake rates also. The rate of nitrate reduction may have limited the supply of nitrogen available for further assimilation. Internal nitrate concentrations were inversely correlated with nitrate uptake rates. Except for ammonium-grown cultures, internal total amino acids and soluble protein showed no correlation with uptake rates. Both internal pool concentrations and enzyme activities are required to interpret changes in uptake rate during growth.

Keywords

Uptake Rate Nitrate Reductase Activity Rhodophyta Nitrate Uptake Total Amino Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature cited

  1. Best, E. P. H.: Effects of nitrogen on the growth and nitrogenous compounds of Ceratophyllum demersum. Aquat. Bot. 8, 197–206 (1980)Google Scholar
  2. Bird, K. T., C. Habig and T. DeBusk: Nitrogen allocation and storage patterns in Gracilaria tikvahiae (Rhodophyta). J. Phycol. 18, 344–348 (1982)Google Scholar
  3. Buggeln, R. G.: Physiological investigations of Alaria esculenta (L.) Grev. (Laminariales). I. Elongation of the blade. J. Phycol. 10, 283–288 (1974)Google Scholar
  4. Chapman, A. R. O. and J. S. Craigie: Seasonal growth in Laminaria longicrusis: relations with dissolved inorganic nutrients and internal reserves of nitrogen. Mar. Biol. 40, 197–205 (1977)Google Scholar
  5. Conway, H. L.: Interactions of inorganic nitrogen in the uptake and assimilation by marine phytoplankton. Mar. Biol. 39, 221–232 (1977)Google Scholar
  6. Conway, H. L., P. J. Harrison and C. O. Davis: Marine diatoms grown in chemostats under silicate or ammonium limitation. II. Transient response of Skeletonema costatum to a single addition of the limiting nutrient. Mar. Biol. 35, 187–199 (1976)Google Scholar
  7. Davis, C. O., P. J. Harrison and R. C. Dugdale: Continuous culture of marine diatoms under silicate limitation. I. Synchronized life cycle of Skeletonema costatum. J. Phycol. 9, 175–180 (1973)Google Scholar
  8. DeBoer, J. A., H. J. Guigli, T. L. Israel and C. F. D'Elia: Nutritional studies of two red algae. I. Growth rate as a function of nitrogen source and concentration. J. Phycol. 14, 261–266 (1978)Google Scholar
  9. DeElia, C. F. and J. A. DeBoer: Nutritional studies of two red algae. II. Kinetics of ammonium and nitrate uptake. J. Phycol. 14, 266–272 (1978)Google Scholar
  10. Dortch, Q., S. I. Ahmed, T. T. Packard: Nitrate reductase and glutamate dehydrogenase activities in Skeletonema costatum as a measure of N assimilation rates. J. Plankton Res. 1, 169–186 (1979)Google Scholar
  11. Dortch, Q.: Nitrate and ammonium uptake and assimilation by three marine diatoms, 299 pp. Ph.D. thesis, University of Washington, Seattle, USA 1980Google Scholar
  12. Dortch, Q., J. R. Clayton, Jr., S. S. Thoresen, S. L. Bressler and S. T. Ahmed: Response of marine phytoplankton to nitrogen deficiency: decreased nitrate uptake vs enhanced ammonium uptake. Mar. Biol. 70, 13–19 (1982)Google Scholar
  13. Eppley, R. W., J. L. Coatsworth and L. Solórzano: Studies of nitrate reductase in marine phytoplankton. Limnol. Oceanogr. 14, 194–205 (1969)Google Scholar
  14. Gagné, J. A., K. H. Mann and A. R. O. Chapman: Seasonal patterns of growth and storage in Laminaria longicruris in relation to differing patterns of availability of nitrogen in the water. Mar. Biol. 69, 91–101 (1982)Google Scholar
  15. Gerard, V. A.: Growth and utilization of internal nitrogen reserves by the giant kelp Macrocystis pyrifera in a low-nitrogen environment. Mar. Biol. 66, 27–35 (1982)Google Scholar
  16. Gordon, D. M., P. B. Birch and A. J. McComb: Effects of inorganic phosphorus and nitrogen on the growth of an estuarine Cladophora in culture. Botanica mar. 24, 93–106 (1981)Google Scholar
  17. Guillard, R. R. L. and J. H. Ryther: Studies on marine planktonic diatoms. I. Cyclotella nana (Hustedt) and Detonula confervaceae (Cleve) Gran. Can. J. Microbiol. 8, 229–239 (1962)Google Scholar
  18. Haines, K. C. and P. A. Wheeler: Ammonium and nitrate uptake by the marine macrophytes Hypnea musciformis (Rhodophyta) and Macrocystis pyrifera (Phaeophyta). J. Phycol. 14, 319–324 (1978)Google Scholar
  19. Hanisak, M. D. and M. M. Harlin: Uptake of inorganic nitrogen by Codium fragile subsp. tomentosoides (Chlorophyta). J. Phycol. 14, 450–454 (1978)Google Scholar
  20. Harrison, P. J., L. D. Druchl, K. Lloyd and P. A. Thompson: Nitrogen uptake kinetics in three year classes of Laminaria groenlandica (Laminariales: Phaeophyta). (In preparation)Google Scholar
  21. Lee, Y. P. and T. Takahashi: An improved colorimetric determination of amino acids with ninhydrin. Analyt. Biochem. 14, 71–77 (1966)Google Scholar
  22. Leggett-Bailey, J.: Miscellaneous analytical methods: estimation of protein Folin-Ciocalteu reagent. In: Techniques in protein chemistry, 2nd ed. pp 340–342. New York: Elsevier Publishing Co. 1967Google Scholar
  23. Maestrini, S. Y., J. Robert and I. Truquet: Simultaneous uptake of ammonium and nitrate by oyster-pond algae. Mar. Biol. Lett. 3, 143–153 (1982)Google Scholar
  24. MacKown, C. T., R. J. Volk and W. A. Jackson: Nitrate assimilation by decapitated corn root systems: effects of ammonium during induction. Pl. Sci. Lett. 24, 295–302 (1982)Google Scholar
  25. McCarthy, J. J. and J. C. Goldman: Nitrogenous nutrition of marine phytoplankton in nutrient depleted waters. Sciences, N.Y. 203, 670–672 (1979)Google Scholar
  26. McCarthy, J. J., R. W. Taylor and J. L. Taft: Nitrogenous nutrition of the plankton in Chesapeake Bay. I. Nutrient availability and phytoplankton preferences. Limnol. Oceanogr. 22, 996–1011 (1977)Google Scholar
  27. Morris, I. and P. J. Syrett: The effect of nitrogen starvation on the activity of nitrate reductase and other enzymes in Chlorella. J. gen. Microbiol. 38, 21–28 (1965)Google Scholar
  28. Nicholas, D. J. D.: Metallo-enzymes in nitrate micro-organisms. Symp. Soc. exp. Biol. 13, 1–13 (1959)Google Scholar
  29. Probyn, T. A. and A. R. O. Chapman: Nitrogen uptake characteristics of Chordaria flagelliformis (Phaeophyta) in batch mode and continuous mode experiments. Mar. Biol. 71, 129–133 (1982)Google Scholar
  30. Rosenberg, G. and J. Ramus: Ecological growth strategies in the seaweeds Gracilaria foliifera (Rhodophyceae) and Ulva sp. (Chlorophyceae): soluble nitrogen and reserve carbohydrates. Mar. Biol. 66, 251–259 (1982)Google Scholar
  31. Syrett, P. J.: Nitrogen assimilation. In: Physiology and biochemistry of algae, pp 171–188. Ed. by R. A. Lewin. New York: Academic Press 1962Google Scholar
  32. Thomas, T. E.: Ecological aspects of nitrogen uptake in intertidal macrophytes, 207 pp. Ph.D. thesis, University of British Columbia, Vancouver, B.C., Canada 1983Google Scholar
  33. Thomas, T. E. and P. J. Harrison. In vitro and in vivo nitrate reductase activity in three intertidal seaweeds. (In preparation)Google Scholar
  34. Turpin, D. H. and P. J. Harrison: Limiting nutrient patchiness and its role in phytoplankton ecology. J. exp. mar. Biol. Ecol. 39, 151–166 (1979)Google Scholar
  35. Veidner, M. and H. Kiefer: Nitrate reduction in the marine brown alga Giffordia mitchellae (Harv.) Ham. Z. PflPhysiol. 104, 341–351 (1981)Google Scholar
  36. Wheeler, W. N. and L. M. Srivastava: Seasonal nitrate physiology of Macrocystis intergrfolia Bory. J. exp. mar. Biol. Ecol. 76, 35–50 (1984)Google Scholar
  37. Yamada, N.: Studies on the manure for seaweed. I. On the change of nitrogenous component of Gelidium amansii Lmx. cultured with different nitrogen sources. Bull. Jap. Soc. scient. Fish. 27, 953–957 (1961)Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • T. E. Thomas
    • 1
  • P. J. Harrison
    • 1
  1. 1.Department of OceanographyUniversity of British ColumbiaVancouverCanada

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