Abstract
Biologically-active substances were investigated from 35 demosponge species and 5 calcareous sponge species collected from intertidal or shallow subtidal habitats near San Diego, California, USA, from 1978 to 1980. Crude methanolic extracts of each species were tested for suppression of growth by bacteria (7 species) and a yeast. Antimicrobial activity was found in 26 Demospongiae and 2 Calcarea. Strong activity was found in 11 demosponges and, subsequently, 38 natural products with antimicrobial activity were isolated from 8 of these species (Aplysina fistularis, Dysidea amblia, Leiosella idia, Euryspongia sp., Toxadocia zumi, Axinella sp., Haliclona?cinerea and ?Pachychalina lunisimilis). Twenty-eight of these natural products (usually as pure compounds) were assayed for: (1) suppression of growth of marine fungi (3 spp.) and a red alga; (2) behavior modifications of invertebrate adults (4spp.); (3) toxicity to a goldfish; (4) inhibition of sexual reproduction of a brown alga; (5) inhibition of settlement and/or metamorphosis of late larvae or invertebrate juveniles (4spp.). Many of the natural products were also incorporated into pelleted fish food and tested for feeding-behavior modifications of fishes (5 spp.). Three of the compounds from Dysidea amblia were inactive in all tests. All other natural products were active in at least one assay, although none was active in all assays. The discussion relates the possession of biologically-active substances to the ecology of each sponge species; for example, sponges with antimicrobial substances are rarely overgrown.
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Literature cited
Amade, Ph., D. Pesando and L. Chevolot: Antimicrobial activities of marine sponges from French Polynesia and Brittany. Mar. Biol. 70, 223–228 (1982)
Ayling, A. L.: Population biology and competitive interactions in subtidal sponge dominated communities of temperate waters, 113 pp. Ph. D. thesis, University of Auckland 1978
Ayling, A. L.: Factors affecting the spatial distributions of thinly encrusting sponges from temperate waters. Oecologia (Berlin) 60, 412–418 (1983)
Bakus, G. J.: Marine poeciloscleridan sponges of the San Juan Archipelago, Washington. J. Zool., Lond. 149, 415–531 (1966)
Bergquist, P. R. and J. J. Bedford: The incidence of antibacterial activity in marine Demospongiae; systematic and geographic considerations. Mar. Biol. 46, 215–221 (1978)
Bergquist, P. R. and R. J. Wells: Chemotaxonomy of the Porifera: the development and current status of the field. In: Marine natural products, Vol. 5. pp 1–50. Ed. by P. J. Scheuer. New York: Academic Press 1983
Burkholder, P. R. and K. Ruetzler: Antimicrobial activity of some marine sponges. Nature, Lond. 222, 983–984 (1969)
Dickinson, M. G.: Sponges of the Gulf of California. Allan Hancock Pacif. Exped. 11, 1–251 (1945)
Green, G.: Ecology of toxicity in marine sponges. Mar. Biol. 40, 207–215 (1977)
Jackson, J. B. C. and L. Buss: Allelopathy and spatial competition among coral reef invertebrates. Proc. natn. Acad. Sci. U.S.A. 72, 5160–5163 (1975)
Johnson, T. W., Jr. and F. K. Sparrow, Jr. Fungi in oceans and estuaries, 668 pp. Weinheim, F. R. G.: Cramer 1961
Kittredge, J. S.: Behavioral bioassays and biologically active compounds. In: Food-drugs from the sea, Proceedings 1974, pp 467–475. Ed. by H. H. Webber and G. D. Ruggieri. Marine Technology Society 1976
Koltun, V. M.: Corneosiliceous sponges of the Northern and Far Eastern Seas of the USSR. Opred. Faune SSSR. 67, 1–235 (1959)
Lambe, L. M.: On some sponges from the Pacific Coast of Canada and Behring Sea. Proc. Trans. R. Soc. Can. 10, 67–78 (1892)
Lambe, L. M.: Sponges from the Pacific coast of Canada. Proc. Trans. R. Soc. Can. 11, 25–43 (1893)
Lambe, L. M.: Sponges from the western coast of North America. Proc. Trans. R. Soc. Can. 12, 113–138 (1894)
Lambe, L. M.: Catalogue of the recent sponges of Canada and Alaska. Ottawa Nat. 14, 153–172 (1900)
Laubenfels, M. W. de: The marine and fresh-water sponges of California. Proc. U.S. natn. Mus. 81, 1–140 (1932)
Laubenfels, M. W. de: A discussion of the sponge fauna of the Dry Tortugas in particular and West Indies in general, with materials for a revision of the families and orders of the Porifera. Pap. Tortugas Lab. 30, 1–225 (1936)
Moody, K., R. H. Thomson, E. Fattorusso, L. Minale and G. Sodano: Aerothionin and homoaerothionin: two tetrabromo spirohexadienyl-isoxazoles from Verongia species. J. chem. Soc. Perkin Trans. 1972 (2), 18–24 (1972)
Morse, D. E., N. Hooker, H. Duncan and L. Jensen: γ-aminobutyric acid, a neurotransmitter, induces planktonic abalone larvae to settle and begin metamorphosis. Science, N.Y. 204, 407–410 (1979)
Nakatsu, T., R. P. Walker, J. E. Thompson and D. J. Faulkner: Biologically-active sterol sulfates from the marine sponge Toxadocia zumi. Experientia 39, 759–761 (1983)
Nealson, K.: Isolation and identification of luminous bacteria In: Methods in enzymology, pp 153–156. Ed. by M. DeLuca. New York: Academic Press (1978)
Paul, V. J., O. J. McConnell and W. Fenicall: Cyclic monoterpenoid feeding deterrents from the red marine alga Ochtodes crockeri. J. org. Chem. 45, 3401–3407 (1980)
Provasoli, L.: Media and prospects for the cultivation of marine algae. In: Cultures and collections of algae, pp 63–75. Ed. by A. Watanabe and A. Hattori. Tokyo: Japan Society of Plant Physiologists 1968
Ristau, D. A.: Shallow-water Demospongiae of north central California: taxonomy and systematics, distribution and fossilization potential, 187 pp. Ph.D. thesis, University of California, Davis 1977
Sokal, R. R. and F. J. Rohlf: Biometry. The principles and practice of statistics in biological research, 776 pp. San Francisco: W. H. Freeman & Co. 1969
Sun, H. H. and W. Fenical: Rhipocephalin and rhipocephenal toxic feeding deterrents from the tropical marine alga (Rhipocephalus phoenix). Tetrahedron Lett. 1979, 685–688 (1979)
Targett, N. M.: Gastropod tentacle withdrawal: a screening procedure for biological activity in marine macroalgae. Botanica mar. 22, 543–545 (1979)
Thompson, J. F.: Chemical ecology and the structure of sponge dominated assemblages, 332pp. Ph.D. thesis, University of California, San Diego 1984
Thompson, J. E.: Exudation of biologically-active metabolites in the sponge Aplysina fistularis. I. Biological evidence. Mar. Biol. 88, 23–26 (1985)
Thompson, J. E., R. P. Walker, S. J. Wratten and D. J. Faulkner: A chemical defense mechanism for the nudibranch Cadlina luteomarginata. Tetrahedron 38, 1865–1873 (1982)
Walker, R. P.: The chemical ecology of some sponges and nudibranchs from San Diego, 177 pp. Ph.D. thesis, University of California, San Diego 1981
Walker, R. P. and D. J. Faulkner: Diterpenes from the sponge Dysidea amblia. J. org. Chem. 46, 1098–1102 (1981)
Walker, R. P., J. E. Thompson and D. J. Faulkner: Sesterterpenes from Spongia idia. J. org. Chem. 45, 4976–4979 (1980)
Walker, R. P., J. E. Thompson and D. J. Faulkner: Exudation of biologically-active metabolites in the sponge Aplysina fistularis. II. Chemical evidence. Mar. Biol. 88, 27–32 (1985)
Wilkinson, C. R., M. Nowak, B. Austin and R. R. Colewell: Specificity of bacterial symbionts in Mediterranean and Great Barrier Reef sponges. Microb. Ecol. 7, 13–21 (1981)
Wollacott, R. M. and R. L. Zimmer: Metamorphosis of cellularoid bryozoans. In: Settlement and metamorphosis of marine invertebrate larvae, pp 49–63. Ed. by F.-S. Chia and M. E. Rice. New York: Elsevier 1977.(Proceedings of Symposium on Settlement and Metamorphosis of Marine Invertebrate Larvae, American Zoological Society Meeting, Toronto, Ontario, Canada, December 27–28)
Zaro, B. A.: Marine sponges: a source of novel antibiotics. Proc. west. Pharmac. Soc. 25, 11–13 (1982)
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Thompson, J.E., Walker, R.P. & Faulkner, D.J. Screening and bioassays for biologically-active substances from forty marine sponge species from San Diego, California, USA. Mar. Biol. 88, 11–21 (1985). https://doi.org/10.1007/BF00393038
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DOI: https://doi.org/10.1007/BF00393038