Skip to main content
Log in

Functional and environmental anaerobiosis in the razor-clam Ensis directus (Mollusca: Bivalvia)

  • Published:
Marine Biology Aims and scope Submit manuscript

Abstract

In Ensis directus (Conrad) metabolic changes accompanying muscular activity and experimental anoxia were investigated. Octopine dehydrogenase, strombine dehydrogenase, alanopine dehydrogenase and lactic dehydrogenase were shown to be present in different parts of the body, but the activity of ODH always exceeded that of the other enzymes by far. Muscular activity connected with “escape digging” or evoked by electrical stimulation resulted in a decrease of stored phosphagen and in an accumulation of octopine. During recovery the normal concentrations of energy-rich phosphates were restored quickly, whereas octopine was removed more slowly. In experimental anoxia only minor quantities of L-and D-alanine, succinate, propionate, and acetate were formed. Quantitatively the most important endproduct was strombine, which rose linearly with the duration of anoxic incubation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Literature cited

  • Baldwin, J. and W. R. England: The properties and functions of alanopine dehydrogenase and octopine dehydrogenase from ghe pedal retractor muscle of Strombidae. Pacif. Sci. 36, 381–394 (1982)

    Google Scholar 

  • Bergmeyer, H. U.: Methoden der enzymatischen Analyse, 3. Aufl. Bd. I, pp 1–1170; Bd. II, pp 1171–2353. Weinheim: Verlag Chemie 1974

    Google Scholar 

  • Drew, G. A.: The habits and movements of the razor-clam, Ensis directus. Biol. Bull. mar. biol. Lab., Woods Hole 12, 127–140 (1907)

    Google Scholar 

  • Eberlee, J. C., J. M. Storey and K. B. Storey: Anaerobiosis, recovery from anoxia and the role of strombine and alanopine in the oyster, Crassostrea virginica. Can. J. Zool. 61, 2682–2687 (1983)

    Google Scholar 

  • Ellington, W. R.: Metabolism of the pyruvate branchpoint in the radula retractor muscle of the whelk, Busycon contrarium. Can. J. Zool. 60, 2973–2977 (1982)

    Google Scholar 

  • Fields, J. H. A., A. K. Eng, W. D. Ramsden and P. W. Hochachka: Alanopine and strombine are novel imino acids produced by a dehydrogenase found in the adductor muscle of the oyster, Crassostrea gigas. Arch. Biochem. Biophys. 201, 110–114 (1980)

    Google Scholar 

  • Fields, J. H. A. and P. W. Hochachka: Purification and properties of alanopine dehydrogenase of the adductor muscle of the oyster, Crassostrea gigas. Eur. J. Biochem. 114, 615–621 (1981)

    Google Scholar 

  • Foreman, R. A. and W. R. Ellington: Effect of inhibitors and substrate supplementation of anaerobic energy metabolism in the ventricle of the oyster, Crassostrea virginica. Comp. Biochem. Physiol. 74 B, 543–547 (1983)

    Google Scholar 

  • Fraenkel, G.: Die Grabbewegungen der Soleniden. Z. vgl. Physiol. 6, 168–220 (1927)

    Google Scholar 

  • Gäde, G.: The energy metabolism of the foot muscle of the jumping cockle, Cardium tuberculatum: sustained anoxia versus muscular activity. J. comp. Physiol. 137, 177–182 (1980)

    Google Scholar 

  • Gäde, G. and M. Grieshaber: Pyruvate reductases catalyze the formation of lactate and opines in anaerobic invertebrates. Comp. Biochem. Physiol. 83B, 255–272 (1986)

    Google Scholar 

  • Gäde, G. and K. H. Carlsson: Purification and characterisation of octopine dehydrogenase from the marine nemertean Cerebratulus lacteus (Anopla: Heteronemerta): comparison with scallop octopine dehydrogenase. Mar. Biol. 79, 39–45 (1984)

    Google Scholar 

  • Gäde, G., E. Weeda and P. A. Gabbott: Changes in the level of octopine during escape responses of the scallop, Pecten maximus. J. comp. Physiol. 124, 121–127 (1978)

    Google Scholar 

  • Grieshaber, M.: Breakdown and formation of high-energy phosphates and octopine in the adductor muscle of the scallop, Chlamys opercularis, during escape swimming and recovery. J. comp. Physiol. 126, 269–276 (1978)

    Google Scholar 

  • Grieshaber, M. and G. Gäde: Energy supply and formation of octopine in the adductor muscle of the scallop, Pecten jacobaeus. Comp. Biochem. Physiol. 58B, 249–252 (1977)

    Google Scholar 

  • Grieshaber, M., E. Kronig and R. Koormann: A photometric estimation of phospho-L-arginine, arginine and octopine using octopine dehydrogenase isoenzyme 2 from the squid, Loligo vulgaris. Hoppe-Seylers Z. Physiol. Chem. 359, 133–136 (1978)

    Google Scholar 

  • Kluytmans, J. H., P. R. Veenhof and A. de Zwaan: Anaerobic production of volatile fatty acids in the sea-mussel, Mytilus edulis. J. comp. Physiol. 104, 71–78 (1975)

    Google Scholar 

  • Livingstone, D. R., A. de Zwaan and R. J. Thompson: Aerobic metabolism, octopine production and phosphoarginine as sources of energy in the phasic and catch adductor muscle of the giant scallop, Placopecten magellanicus during swimming and the subsequent recovery period. Comp. Biochem. Physiol. 70B, 35–44 (1981)

    Google Scholar 

  • Meinardus, G. and G. Gäde: The pyruvate branchpoint in the anaerobic metabolism of the jumping cockle, Cardium tuberculatum. Exp. Biol. 45, 91–110 (1986)

    Google Scholar 

  • Michal, G. H., H. O. Beutler, G. Land and U. Guenter: Enzymatic determination of succinic acid in food-stuffs. J. anal. Chem. 279, 137–138 (1976)

    Google Scholar 

  • Nicchitta, C. V. and W. R. Ellington: Energy metabolism during air exposure and recovery in the high intertidal bivalve mollusc, Geukensia demissa and the subtidal bivalve mollusc, Modiolus squamosus. Biol. Bull. mar. biol. Lab., Woods Hole 165, 708–722 (1983)

    Google Scholar 

  • Schöttler, U.: On the anaerobic metabolism of three species of Nereis (Annelida). Mar. Ecol. Prog. Ser. 1, 249–254 (1979)

    Google Scholar 

  • Siegmund, B. and M. Grieshaber: Determination of mesoalanopine and D-strombine by HPLC in extracts from marine invertebrates. Hoppe-Seylers Z. Physiol. Chem. 364, 807–812 (1983)

    Google Scholar 

  • Siegmund, B., M. Grieshaber, M. Reitze and E. Zebe: Alanopine and strombine are endproducts of the anaerobic glycolysis in the lugworm, Arenicola marina (Annelida, Polychaeta). Comp. Biochem. Physiol. 82B, 337–345 (1985)

    Google Scholar 

  • Trueman, E. R.: The dynamics of burrowing in Ensis (Bivalvia). Proc. R. Soc. Lond. Ser. B. 166, 459–476 (1967)

    Google Scholar 

  • Zurburg, W., A. M. T. de Bont and A. de Zwaan: Recovery from exposure to air and the occurrence of strombine in different organs of the sea-mussel, Mytilus edulis. Mol. Physiol. 2, 135–147 (1982)

    Google Scholar 

  • Zwaan, A. de: Carbohydrate catabolism in bivalves. In: The mollusca, vol. 1, pp 137–175. Ed. by P. W. Hochachka. New York: Academic Press 1983

    Google Scholar 

  • Zwaan, A. de, A. M. T. de Bont and J. Hemelraad: The role of phosphoenolpyruvate carboxykinase in the anaerobic metabolism of the sea-mussel, Mytilus edulis. J. comp. Physiol. 153, 267–274 (1983a)

    Google Scholar 

  • Zwaan, A. de, A. M. T. de Bont, W. Zurburg, B. L. Bayne and D. R. Livingstone: On the role of strombine formation in the energy metabolism of the adductor muscle of a sessile bivalve. J. comp. Physiol. 149, 557–563 (1983b)

    Google Scholar 

  • Zwaan, A. de and P. R. Dando: Phosphoenolpyruvate metabolism in bivalve molluscs. Mol. Physiol. 8, 285–310 (1984)

    Google Scholar 

  • Zwaan, A. de, R. J. Thompson and D. R. Livingstone: Physiological and biochemical aspects of valve snap and valve closure responses in the giant scallop, Placopecten magellanicus. II. Biochemistry. J. comp. Physiol. 137, 105–115 (1980)

    Google Scholar 

  • Zwaan, A. de and W. Zurburg: The formation of strombine in the adductor muscle of the sea-mussel, Mytilus edulis. Mar. Biol. Lett. 1, 179–192 (1981)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by O. Kinne, Oldendorf/Luhe

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schiedek, D., Zebe, E. Functional and environmental anaerobiosis in the razor-clam Ensis directus (Mollusca: Bivalvia). Mar. Biol. 94, 31–37 (1987). https://doi.org/10.1007/BF00392897

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00392897

Keywords

Navigation