Abstract
Nitrite excretion during nitrate uptake by three nitrate-limited diatoms was measured at different temperatures. Phaeodactylum tricornutum Bohlin and Chaetoceros affinis Lauder Hustedt excreted nitrite over the whole range of physiological temperatures, whereas Thalassiosira pseudonana Hasle and Heimdal (Clone 13-1) excreted nitrite only at 25°C. Parallel to growth and nitrate-uptake rates, excretion rates increased exponentially with temperature, attaining a maximum between 20° and 25°C (optimum temperature range). At the end of nitrate uptake, when the nitrate concentration in the culture medium had decreased below 1 μM, nitrite was reabsorbed at all temperatures, except when cells were in the dark or at very low light intensity. Nitrite uptake was also inhibited by the presence of nitrate in the medium. These results are discussed in relation to the formation and position of the maximum layer of primary nitrite in the thermocline, below the maximum layer of chlorophyll in stratified oceanic areas.
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Literature cited
Ahlgren, G.: Growth of Oscillatoria agardhii in chemostat culture. 2. Dependence of growth constants on temperature. Mitt. int. Verein. theor. angew. Limnol. 21, 88–102 (1978)
Anderson, S. M. and O. A. Roels: Effects of light intensity on nitrate and nitrite uptake and excretion by Chaetoceros curvisetus. Mar. Biol. 62, 257–361 (1981)
Caperon, J. and J. Meyer: Nitrogen-limited growth of marine phytoplankton. I. Changes in population characteristics with steady-state growth rate. Deep-Sea Res. 19, 601–618 (1972)
Carlucci, A. F., E. O. Hartwig and P. M. Bowes: Biological production of nitrite in sea water. Mar. Biol. 7, 161–166 (1970)
Collos, Y.: Transient situations in nitrate assimilation by marine diatoms. 2. Changes in nitrate and nitrite following a nitrate perturbation. Limnol. Oceanogr. 27, 528–535 (1982)
Collos, Y. and G. Slawyk: 13C and 15N uptake by marine phytoplankton. 1. Influence of the nitrogen source and concentration in laboratory cultures of diatoms. J. Phycol. 15, 186–190 (1979)
Collos, Y. and G. Slawyk: Ammonium and nitrate in the Tropical Equatorial Atlantic: relations with the primary nitrite maximum. Mar. Biol. Lett. 4, 295–308 (1983)
Conover, S. A. M.: Partitioning of nitrogen and carbon in cultures of the marine diatom Thalassiosira fluviatilis supplied with nitrate, ammonium, or urea. Mar. Biol. 32, 231–246 (1975)
Conway, H. L., P. J. Harrison and C. O. Davis: Marine diatoms grown in chemostats under silicate or ammonium limitation. II. Transient response of Skeletonema costatum to a single edition of the limiting nutrient. Mar. Biol. 35, 187–199 (1976)
Dugdale, R. C.: Nutrient limitation in the sea: dynamics, identification and significance. Limnol. Oceanogr. 12, 685–695 (1967)
Eppley, R. W. and J. L. Coatsworth: Uptake of nitrate and nitrite by Dytilum brightwellii. Kinetics and mechanisms. J. Phycol. 4, 151–156 (1968)
Groupe Médiprod: Campagne Guidome, 1976. Publs Cent. natn. Exploit. Océans (CNEXO) (Sér. Résult. Campagnes mer) 13, 1–103 (1977)
Guillard, R. R. L. and J. H. Ryther: Studies of marine planktonic diatoms. I. Cyclotella nana (Hustedt) and Detonula confervacea (Cleve). Gran. Can. J. Microbiol. 8, 229–239 (1962)
Harrison, P. J. and C. O. Davis: Use of the perturbation technique to measure nutrient uptake rates of natural phytoplankton populations. Deep-Sea Res. 24, 247–255 (1977)
Hattori, A. and E. Wada: Nitrite distribution and its regulating process in the Equatorial Pacific Ocean. Deep-Sea Res. 18, 557–568 (1971)
Herbland, A. et B. Voituriez: Production primaire, nitrate et nitrite dans l'atlantique tropical. I. Distribution du nitrate et production primaire. Cah. O.R.S.T.O.M. Sér. Océanogr. 15, 47–55 (1977)
Hisard, P. et B. Piton: La distribution du nitrite dans le système des courants équatoriaux de l'océan pacifique à 170 E. J. Cons. perm. int. Explor. Mer 32, 303–317 (1969)
Kaufman, J. E., J. B. Beard and D. Penner: The influence of temperature on nitrate reductase activity of Agrostis palustris and Cynodon dactylon. Physiologia Pl. 25, 378–381 (1971)
Kiefer, D. A., R. I. Olson and O. Holm-Hansen: Another look at the nitrite and chlorophyll maxima in the Central North Pacific. Deep-Sea Res. 23, 1199–1208 (1976)
Lui, N. S. and O. A. Roels: Nitrogen metabolism of aquatic organisms. 2. The assimilation of nitrate, nitrite and ammonia by Biddulphia aurita. J. Phycol. 8, 259–264 (1972)
Miyasaki, T., E. Wada and A. Hattori: Nitrite production of ammonia and nitrate in the euphotic layer of the Western North Pacific Ocean. Mar. Sci. Communs 1, 381–394 (1975)
Olson, R. J., J. S. Soohoo and D. A. Kiefer: Steady-state growth of the marine diatom Thalassiosira pseudonana: the uncoupled kinetics of nitrate uptake and nitrite production. Pl. Physiol., Baltimore, Md 66, 383–389 (1980)
Parslow, J. S., P. J. Harrison and P. A. Thompson: Saturated uptake kinetics: transient responses of the marine diatom Thalassiosira pseudonana to ammonium, nitrate, silicate or phosphate starvation. Mar. Biol. 83, 51–59 (1984)
Raimbault, P.: Influence de la temperature sur la cinétique d'assimilation et le taux de croissance du phytoplancton, 96 pp. Thèse de 3ème cycle, Université d'Aix-Marseille II. 1982
Raimbault, P.: Influence of temperature on the transient response in nitrate uptake and reduction by four marine diatoms. J. exp. mar. Biol. Ecol. 84, 37–53 (1984)
Ricketts, T. R. and P. A. Edge: Nitrate and nitrite reductases in Platymonas striata Butcher (Prasinophyceae). Br. phycol. J. 13, 167–176 (1978)
Sorokin, C.: Kinetic studies of temperature effects on the cellular level. Biochim. biophys. Acta 38, 197–204 (1960)
Tréguer, P. and P. Le Corre: Manuel d'analyse des sels nutritifs dans l'eau de mer (utilisation de l'autonalyser II Technicon), 2ème éd. 110 pp. Brest: Laboratoire d'Océanographie Chimique, Université de Bretagne Occidentale 1975
Vaccaro, R. F. and J. H. Ryther: Marine phytoplankton and the distribution of nitrite in the sea. J. Cons. perm. int. Explor. Mer 25, 260–271 (1960)
Voituriez, B. et A. Herbland: Production primaire, nitrate et nitrite dans l'atlantique tropical. II. Distribution du nitrate et production du nitrite. Cah. O.R.S.T.O.M. Sér. Océanogr. 15, 57–65 (1977)
Wada, E. and A. Hatori: Nitrite metabolism in the euphotic layer of the Central North Pacific Ocean. Limnol. Oceanogr. 16, 766–772 (1971)
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Communicated by J. M. Pérès, Marseille
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Raimbault, P. Effect of temperature on nitrite excretion by three marine diatoms during nitrate uptake. Marine Biology 92, 149–155 (1986). https://doi.org/10.1007/BF00392831
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DOI: https://doi.org/10.1007/BF00392831