Abstract
We assessed the utility of separated carotenoid mixtures for sponge systematics. Carotenoids were extracted from 29 species of 22 genera of 6 families for the demosponge order Poecilosclerida. Mixtures were separated (by thinlayer chromatography on silica gel) without chemical modification and after alteration by saponification, reduction or acetylation or a combination thereof. This approach allowed analysis of individual colonies of less than 1 g wet weight. Relationships among taxa were determined through use of Lawson's similarity index and discriminative analysis. Analysis of multiple runs of fractions from one individual of Ophlitaspongia pennata showed the method to be consistent and reliable. Comparison of specimens of species collected at different localities and times showed high correspondence of carotenoid patterns except in those species suspected of representing species complexes. Analysis of members of the family Clathriidae and of relationships within the order Poecilosclerida suggest that fatty acids esterified to carotenoids may be family-specific and of more taxonomic value than carotenoids. The importance of testing for seasonal, geographic and habitat variability in biochemical patterns is stressed. We discuss the significance of these methods to sponge systematics and their use in comparative studies.
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Literature cited
Bergmann, W.: Comparative biochemical studies on the lipids of marine invertebrates, with special reference to the sterols. J. mar. Res. 8, 137–176 (1949)
Bergmann, W.: Sterols: their structure and distribution. In: Comprehensive biochemistry, Vol. 3 pp 103–162. Ed. by M. Florkin and H. S. Mason. New York: Academic Press 1962
Bergquist, P. R.: Sponges, 268 pp. London: Hutchinson 1978
Bergquist, P. R.: The ordinal and subclass classification of the Demospongiae (Porifera): appraisal of the present arrangement, and proposal of a new order. N. Z. Jl Zool. 7, 1–6 (1980)
Bergquist, P. R. and W. D. Hartman: Free amino acid patterns and the classification of the Demospongiae. Mar. Biol. 3, 247–268 (1969)
Bergquist, P. R., W. Hofheinz and G. Oesterhelt: Sterol composition and the classification of the Demospongiae. Biochem. Syst. Ecol. 8, 423–435 (1980)
Bergquist, P. R. and J. J. Hogg: Free amino acid patterns in Demospongiae: A biochemical approach to sponge classification. Cah. Biol. mar. 10, 105–220 (1969)
Bergquist, P. R., M. P. Lawson, A. Lavis and R. C. Cambie: Fatty acid composition and the classification of the Porifera. Biochem. Syst. Ecol. 13, 63–84 (1984)
Cimino, G., S. deStefano, L. Minale and G. Sodano: Metabolism in Porifera III. Chemical patterns and the classification of the Demospongiae. Comp. Biochem. Physiol 50 B, 279–285 (1975)
Davies, H. B.: Carotenoids. In: Chemistry and biochemistry of plant pigments, Vol. 2. pp 28–165. Ed. by T. W. Goodwin. London: Academic Press 1976
Hartman, W. D.: Phylum Porifera. In: Light's manual: intertidal invertebrates of the central California coast, pp 32–64. Ed. by R. I. Smith and J. T. Carlton. Berkeley: University of California Press 1975
Krinsky, N. I. and T. H. Goldsmith: Carotenoids of the flagellated alga, Euglena gracilis. Archs Biochem. Biophys. 91, 271–279 (1960)
Lawson, R., D. H. Davies, J. Casey and S. A. Mohammad: The interpretation of protein separations on polyacrylamide gels. Lab. Pract. 29, 1065–1066 (1980)
Liaaen-Jensen, S.: Marine carotenoids. In: Marine natural products, chemical and biological perspectives, Vol. 1 pp 1–5 Ed. by P. Scheru. New York: Academic Press 1979
Liaaen-Jensen, S. and A. Jensen: Recent progress in carotenoid chemistry. Recent Prog. Chemy Fats other Lipids 8, 133–212 (1965)
Liaaen-Jensen, S., B. Renstrom, T. Ramdahl, M. Hallenstuet and P. Bergquist: Carotenoids of marine sponges. Biochem. Syst. Ecol. 10, 167–174 (1982)
Lindberg, D. R.: A multivariate study of morphological variation of the lipet Notoacmea depicta (Hinds) and its synonyms Notoacmea gabatella (Berry) and Notoacmea lepisma (Berry) (Gastropoda: Acmaeidae). Bull. Sth. Calif. Acad. Sci. 81, 87–96 (1982)
Lindenfelser, M. E.: Morphometric and allozymic congruence: evolution in the prawn Macrobrachium rosenbergii (Decapoda: Palaemonidae). Syst. Zool. 33, 195–204 (1984)
Litchfield, C. and S. Liaaen-Jensen: Carotenoids of the marine sponge Microciona prolifera. Comp. Biochem. Physiol. 66 B, 359–365 (1980)
Nie, N. H., C. H. Hull, J. G. Jenkins, K. Steinbrenner and D. H. Bent: SPSS statistical package for the social sciences, 2nd ed. xxiv+675 pp. New York: McGraw Hill 1975
Randerath, K.: Thin layer chromatography, 250 pp. New York: Academic Press 1964
Shaklee, J. R. and C. S. Tamaru: Biochemical and morphological evolution of Hawaiian bonefishes (Albula) Syst. Zool. 30,. 125–146 (1981)
Simpson, T. L.: A new species of clathriid sponge from the San Juan Archipelago. Postilla 103, 1–7 (1966)
Simpson, T. L.: The cell biology of sponges, 662 pp. New York: Springer-Verlag 1984
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Communicated by N. D. Holland, La Jolla
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Lee, W.L., Gilchrist, B.M. Carotenoid patterns in twenty-nine species of sponges in the order Poecilosclerida (Porifera: Demospongiae): a possible tool for chemosystematics. Marine Biology 86, 21–35 (1985). https://doi.org/10.1007/BF00392576
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DOI: https://doi.org/10.1007/BF00392576