Abstract
A technique utilizing combined fixation and gentle decalcification has been employed to study the ultrastructure of the vegetative cells of the articulated calcareous coralline algae Corallina officinalis Linnaeus and C. cuvierii Lamouroux (Rhodophyta: Cryptonemiales). The epidermal cells are distinctive, with many cell wall inggrowths which pass between the chloroplasts. It is suggested that these cells function as “transfer cells”. The epidermal cells contain no starch, although the chloroplasts have well developed photosynthetic lamellae. Damage to these epidermal cells leads to formation of new cells by renewed division of sub-epidermal meristematic cells. The outer cortical cells have few small vacuoles and many plastids, with an extensive photosynthetic lamellar system. Deeper into the thallus, the vacuoles increase in size and free cytoplasmic starch grains occur. The medullary cells have a very large vacuole and in older tissue often appear dead. The long genicular cells have calcareous walls at either end while the wall in the middle of these cells is non-calcareous and has an inner fibrillar layer and a thin outer “cuticle”. In partially decalcified material, the orientation of the CaCO3 (calcite) crystals next to the cells can clearly be seen. Immediately next to the cell the crystals are fairly small and arranged at right angles to the plasmalemma. Further away from the cell the crystal size is larger and their orientation is more random. The crystals are surrounded by organic material, and the possible rôle of this material in calcification in coralline algae is discussed.
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Literature Cited
Adey, W.H.: Temperature control of reproduction and productivity in a subartic coralline alga. Phycologia 12, 111–118 (1973)
— and W.H. Johansen: Morphology and taxonomy of Corallinacease with special reference to Clathromorphum, Mesophyllum, and Neopolyporolithon gen nov. (Rhodophyceae, Cryptonemiales) Phycologia 11, 159–180 (1972)
— and I. G McIntyre: Crustose coralline algae: a re-evaluation in the geological sciences. Bull. geol. Soc. Am. 84, 883–904 (1973)
Baas-Becking, A.V. and G. Galliher: Wall structure and mineralization in coralline algae. J. phys. Chem., Wash. 35, 253–274 (1936)
Bailey, A. and T. Bisalputra: A preliminary account of the application of thin-sectioning, freeze-etching, and scanning electron microscopy to the study of coralline algae. Phycologia 9, 83–101 (1970)
Benner, U. und E. Schnepf: Die Morphologie der Nektarausscheidung bei Bromeliaceen: Beteiligung des Golgi-Apparates. Protoplasma 85, 337–349 (1975)
Bisalputra, T., P.C. Rusanowski and W.S. Walker: Surface activity, cell wall, and fine structure of pit connection in the red alga Laurencia spectabilis. J. Ultrastruct. Res. 20, 277–289 (1967)
Borowitzka, M.A.: Algal calcification. Oceanogr. mar. Biol. A. Rev. 15, 189–223 (1977)
— and A.W.D. Larkum: Calcification in the green alga Halimeda. I. An ultrastructure study of thallus development. J. Phycol. 13, 6–16 (1977)
—— and C.E. Nockolds: A scanning electron microscope study of the structure and organization of the calcium carbonate deposits of algae. Phycologia 13, 195–203 (1974)
Bouck, G.B.: Chromatophore development, pits, and other fine structure in the red alga, Lomentaria baileyana (Harv.) Farlow. J. Cell Biol. 12, 553–569 (1962)
Cabioch, J.: Etude sur les corallinacées. I. Charactères généraux de la cytologie. Cah. Biol. mar. 12, 121–186 (1971a)
— Etude sur les corallinacées. II. La morphogenèse; conséquences systématiques et phylogénétiques. Cah. Biol. mar. 13, 137–187 (1971b)
Dawes, C.J., F.M. Scott and E. Bowler: A light-and electron-microscopic survey of algal cell walls. I. Phaeophyta and Rhodophyta. Am. J. Bot. 48, 925–934 (1961)
Dodge, J.D.: The fine structure of algal cells, 261 pp. London: Academic Press 1973
Evans, L.V.: Cytoplasmic organelles. In: Algal physiology and biochemistry, pp 86–123. Ed. by W.D.P. Stewart. Oxford: Blackwell Scientific Publications 1974
Falk, H. und P. Sitte: Zellfeinbau bei Plasmolyse. 1. Der Feinbau der Elodea-Blattzellen. Protoplasma 57, 290–303 (1963)
Gantt, E. and S.F. Conti: The ultrastructure of Porphyridium cruentum. J. Cell Biol. 26, 365–381 (1965)
Giraud, G. et J. Cabioch: Etude ultrastructurale de l'activité des cellules superficielles du thalle des corallinacées (rhodophycées). Phycologia 15, 405–414 (1976)
Gunning, B.E.S. and J.S. Pate: “Transfer cells”: plant cells with wall ingrowths, specialized in relation to short distance transport of solutes—their occurrence, structure, and development. Protoplasma 68, 107–133 (1969)
Hanić, L.A. and J.S. Craigie: Studies on the algal cuticle. J. Phycol. 5, 89–102 (1969)
Hartmann, T. und W. Eschrich: Stofftransport in Rotalgen. Planta 85, 303–312 (1969)
Jagels, R.: Studies of a marine grass, Thalassia testudium. I. Ultrastructure of the osmoregulatory leaf cells. Am. J. Bot. 60, 1003–1009 (1973)
Johansen, H.W.: Morphology and systematics of coralline algae with special reference to Calliarthron. Univ. Calif. Publs Bot. 49, 1–98 (1969)
— Articulated coralline algae. Oceanogr. mar. Biol. A. Rev. 12, 77–127 (1974)
Lee, R.E.: The pit connections of some lower red algae: ultrastructure and phylogenetic significance. Br. phycol. J. 6, 29–38 (1971)
McBride, D.L. and K. Cole: Ultrastructural characteristics of the vegetative cell of Smithora naiadum (Rhodophyta). Phycologia 8, 177–186 (1969)
Naylor, G.L. and B. Russell-Wells: On the presence of cellulose and its distribution in the cell-walls of brown and red algae. Ann. Bot. 48, 635–641 (1934)
Pate, J.S. and B.E.S. Gunning: Transfer cells. A. Rev. Pl. Physiol. 23, 173–196 (1972)
Ramus, J.: Dimorphic pit connections in the red alga Pseudogloiophloea. J. Cell Biol. 41, 340–345 (1969)
— Properties of septal plugs from the red alga Griffithsia pacifica. Phycologia 10, 99–103 (1971)
— The production of extracellular polysaccharide by the unicellular red alga Porphyridium aerugineum. J. Phycol. 8, 97–111 (1972)
Scott, J.L. and P.S. Dixon: Ultrastructure of tetrasporogenesis in the marine red alga Ptilota hypnoides. J. Phycol., 9, 29–46 (1973)
Solms-Laubach, G.: Die Corallinenalgen des Golfes von Neapel und der angrenzenden Meeres-Abschnitte. Fauna Flora Golf. Neapel 4, 1–64, pl. 1-3 (1881)
Spurr, A.R.: A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43 (1969)
Suneson, S.: Studien über die Entwicklungsgeschichte der Corallinaceen. Acta Univ. lund. (N.F. Avd. 2) 33, 1–101 (1937)
— The structure, life-history and taxonomy of the Swedish Corallinaceae. Acta Univ. lund. (N.F. Avd. 2) 39, 1–62 (1943)
Turvey, J.R. and P.R. Simpson: Polysaccharides from Corallina officinalis. Proc. int. Seaweed Symp. 5, 323–327 (1966). (Ed. by E.G. Young and J.L. McLachlan. Oxford: Pergamon Press)
Waaland, S.D., J.R. Waaland: Analysis of cell elongation in red algae by fluorescent labelling. Planta 126, 127–138 (1975)
Yamanouchi, S.: Life history of Corallina officinalis var. mediterranea. Bot. Gaz. 72, 90–96 (1921)
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Communicated by G.F. Humphrey, Sydney
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Borowitzka, M.A., Vesk, M. Ultrastructure of the corallinaceae. I. The vegetative cells of Corallina officinalis and C. cuvierii . Mar. Biol. 46, 295–304 (1978). https://doi.org/10.1007/BF00391400
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DOI: https://doi.org/10.1007/BF00391400