Abstract
Previous attempts to extract high molecular weight DNA from hermatypic corals have failed because the starting material was mature tissue. The following paper describes the isolation of DNA from coral sperm which is of high molecular weight, and is suitable for DNA hybridization, digestion with restriction endonucleases and cloning. Gross DNA hybridization and hybridization with cloned repetitive DNA was used to investigate relatedness amongst members of the scleractinian coral family Acroporidae. Acropora formosa and A. pulchra were found to share a common (highly homologous) DNA sequence repeat; a related but less homologous sequence was present in A. latistella, and no homologous sequence was detected in Montipora digitata. These results imply a close taxonomic relationship between A. formosa and A. pulchra, and suggest that molecular criteria of this sort will be extremely useful in coral taxonomy.
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Babcock, R. C., Bull, G. D., Harrison, P. L., Heyward, A. J., Oliver, J. K., Wallace, C. C., Willis B. L. (1986). Synchronous spawnings of 105 scleractinian coral species on the Great Barrier Reef. Mar. Biol. 90: 379–394
Chambers, A. E., Almond, N. M., Knight, M., Simpson, A. J. G., Parkhouse, R. M. E. (1986). Repetitive DNA as a tool for the identification and comparison of nematode variants: application to Trichinella isolates. Molec. Biochem. Parasit. 21: 113–120
Chirgwin, J. W., Przybyla, A. E., Macdonald, R. J., Rutter, W. J. (1979). Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry (Am. chem. Soc.), Easton, Pa. 18: 5294–5299
Davis, L. G., Dibner, M. D., Battey, J. F. (1986) Basic methods in molecular biology. Elsevier, New York
Dowsett, A. P. (1983). Closely related species of Drosophila can contain different libraries of repetitive DNA sequences. Chromosoma 88: 104–108 (1983)
Harrison, P. L., Babcock, R. C., Bull, G. D., Oliver, J. K., Wallace, C. C., Willis, B. L. (1984). Mass spawning in tropical reef corals. Science, N. Y. 223: 1186–1189
Heyward, A. J., Stoddart, J. A. (1985). Genetic structure of two species of Montipora on a patch reef: conflicting results from electrophoresis and histocompatibility. Mar. Biol. 85: 117–121
Hunt, J. A., Hall, T. J., Britten, R. J. (1981). Evolutionary distances in Hawaiian Drosophila measured by DNA reassociation. J. molec. Evolut. 17: 361–367
Maniatis, T., Fritsch, E. F., Sambrook, J. (1982). Molecular cloning: a laboratory manual. Cold Springer Harbor Press, New York
McDonell, M. W., Simon, M. N., Studier, F. W. (1977). Analysis of restriction fragments of T7DNA and determination of molecular weights in neutral and alkaline gels. J. molec. Biol. 110: 119–146
McLain, D. K., Rai, K. S., Fraser, M. J. (1987). Intraspecific and interspecific variation in the sequence and abundance of highly repeated DNA among mosquitos of the Aedes albopictus subgroup. Heredity, Lond. 58: 373–381
Richardson, B. J., Baverstock, P. R., Adams, M. (1986). Allozyme electrophoresis: a handbook for animal systematics and population studies. Sydney, Academic Press Australia
Rigby, P., Dieckmann, M., Rhodes, C., Berg, P. (1977). Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J. molec. Biol. 113: 237–251
Stoddart, J. A. (1984). Genetic differentiation amongst populations of the coral Pocillopora damicornis off southwestern Australia. Coral Reefs 3: 149–156
Veron, J. E. N., Wallace, C. C. (1984). Scleractinia of eastern Australia Part V: family Acroporidae. Australian National University Press, Canberra
Willis, B. L., Ayre, D. J. (1985). Asexual reproduction and genetic determination of growth form in the coral Pavona cactus: biochemical genetic and immunogenetic evidence. Oecologia (Berlin) 65: 516–525
Willis, B. L., Babcock, R. C., Harrison, P. L., Oliver, J. K., Wallace, C. C. (1985). Patterns in the mass spawning of corals on the Great Barrier Reef from 1981 to 1984. Proc. 5th int. coral Reef Congr. 4: 343–348 [Gabrie, C. et al. (eds.) Antenne Museum-EPHE, Moorea, French Polynesia]
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Communicated by G. F. Humphrey, Sydney
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McMillan, J., Yellowlees, D., Heyward, A. et al. Preparation of high molecular weight DNA from hermatypic corals and its use for DNA hybridization and cloning. Mar. Biol. 98, 271–276 (1988). https://doi.org/10.1007/BF00391205
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DOI: https://doi.org/10.1007/BF00391205