Abstract
Radioactive selenite-75 has been used to investigate the metabolic transformation of inorganic selenium by the marine phytoplankters Tetraselmis tetrathele and Dunaliella minuta. The majority of radioselenium taken up from culture media during growth becomes associated with cellular protein. A small quantity of this protein-bound selenium can be volatilised by treatment with strong acid, suggesting the presence of hydrogen selenide. However, the principal fraction of selenium appears to be integrated into the primary protein structure. Enzymic hydrolysis of phytoplankton protein and subsequent chromatography of hydrolysates revealed the presence of seleno-analogues of the sulphur amino acids. Selenium amino acids were also detected in non-protein extracts.
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Literature Cited
Anonymous: Baseline studies of pollutants in the marine environment and research recommendations, 54 pp. [Presented at The IDOE (International Decade of Ocean Exploration) Baseline Conference May 24–26, 1972]. New York: 1972
Bertine, K.K. and E.D. Goldberg: Fossil fuel consumption and the major sedimentary cycle. Science, N.Y. 173, 223–235 (1971)
Christ, W.D., D. Schmidt and H. Coper: Comparison of thio-NADP and seleno-NADP-dependent oxidoreductases. Hoppe-Seyler's Z. Physiol. Chem. 351, 427–434 (1970)
Diplock, A.T., C.J.P. Caygill, E.H. Jeffrey and C. Thomas: The nature of the acid volatile selenium in the liver of the male rat. Biochem. J. 134, 283–293 (1973)
Fowler, S.W. and G. Benayoun: Selenium kinetics in marine zooplankton. Mar. Sci. Communs 2 (1), 43–67 (1976)
Gamboa-Lewis, B.: Selenium in biological systems and pathways for its volatilisation in higher plants. In: Environmental biogeochemistry. Vol. 1. 389–409. Ed. by J.O. Nriagu. Michigan: Ann Arbour Science 1976
Ganther, H.: Enzymic synthesis of dimethyl selenide from sodium selenite. Biochemistry (Am. chem. Soc.), Easton, Pa. 5, 1089–1098 (1966)
Gissel-Nielsen, G. and M. Gissel-Nielsen: Ecological effects of selenium application to field crops. Ambio 2, 114–117 (1973)
Hamilton, P.B.: Ion exchange chromatography of amino acids. A single column, high resolving, fully automatic procedure. Analyt. Chem. 35, 2055–2064 (1963)
Olson, R.K., Schwarz, M. Horwitt and A.L. Tappell: Nutrition symposium: interrelationships among vitamin E, coenzyme Q and selenium. Fedn Proc. Fedn Am. Socs exp. Biol. 24, 55–92 (1965)
Peterson, P.J. and G.W. Butler: The uptake and assimilation of selenite in higher plants. Aust. J. biol. Sci. 15, 126–146 (1962)
Schrift, A.: A selenium cycle in Nature? Nature, Lond. 201, 1304–1305 (1964)
Thomson, J.F., C.J. Morris and R.M. Zacharius: Isolation of S-methylcysteine from beans, Phaseolus vulgaris. Nature, Lond. 178, p. 593 (1956)
Whanger, P.D., N.D. Pedersen and P.H. Weswig: Selenium protein in ovine tissues. Spectral properties of a 10,000 molecular weight in selenium protein. Biochem. biophys. Res. Commun. 53, 1031–1036 (1973)
Wrench, J.J.: Biochemical aspects of the uptake of mercury and selenium by the native British oyster, Ostrea edulis, 104 pp. Ph.D. thesis, University of Southampton 1977
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Communicated by J.M. Pérès, Marseille
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Wrench, J.J. Selenium metabolism in the Marine phytoplankters Tetraselmis tetrathele and Dunaliella minuta . Marine Biology 49, 231–236 (1978). https://doi.org/10.1007/BF00391135
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DOI: https://doi.org/10.1007/BF00391135